公开(公告)号：US20210010073A1

公开(公告)日：2021-01-14

申请号：US16609869

申请日：2019-03-20

申请人： Illumina, Inc.

发明人： Hongxia Xu ,  Dan Cao ,  Alex Aravanis

IPC分类号： C12Q1/6869 ,  C12Q1/6806 ,  C12N9/12 ,  C40B40/08

摘要： Some embodiments of the methods and compositions provided herein relate to the preparation and use of nucleic acid libraries derived from RNA and DNA. In some embodiments, a nucleic acid library can be prepared by tagging polynucleotides derived from RNA. Some embodiments include the analysis of sequence data from such libraries.

公开(公告)号：US09926598B2

公开(公告)日：2018-03-27

申请号：US14575808

申请日：2014-12-18

申请人： Illumina, Inc.

发明人： Hongxia Xu ,  Alex Aravanis ,  Shengrong Lin

IPC分类号： C12Q1/68 ,  C12N15/10

CPC分类号： C12Q1/6874 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2535/122 ,  C12Q2537/159 ,  C12Q2565/543

摘要： The present disclosure relates to the field of molecular biology and more specifically to methods for capturing, amplifying and sequencing target polynucleotides on a solid surface.

公开(公告)号：US20150197798A1

公开(公告)日：2015-07-16

申请号：US14575808

申请日：2014-12-18

申请人： ILLUMINA, INC.

发明人： Hongxia Xu ,  Alex Aravanis ,  Shengrong Lin

IPC分类号： C12Q1/68 ,  C12N15/10

CPC分类号： C12Q1/6874 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2535/122 ,  C12Q2537/159 ,  C12Q2565/543

摘要： The present disclosure relates to the field of molecular biology and more specifically to methods for capturing, amplifying and sequencing target polynucleotides on a solid surface.

摘要翻译： 本公开涉及分子生物学领域，更具体地涉及在固体表面上捕获，扩增和测序靶多核苷酸的方法。

公开(公告)号：US20240167020A1

公开(公告)日：2024-05-23

申请号：US18549343

申请日：2022-03-08

申请人： Illumina, Inc.

发明人： Hongxia Xu ,  Tong Liu ,  Shi Min Xiao ,  Dan Cao ,  Victor Quijano ,  Kai-How Farh ,  Mohan Sun

IPC分类号： C12N15/10

CPC分类号： C12N15/1082 ,  C12N15/1062 ,  C12N15/1065

摘要： Analyzing expression of protein-coding variants in cells is provided herein. A method may include replacing a protein coding-region of the DNA in a cell with a donor vector including a variant of the protein-coding region and a first barcode identifying that variant. The cell may generate mRNA including an expression of the variant and an expression of the first barcode. A second barcode corresponding to the cell may be coupled to the mRNA. The mRNA. having the second barcode coupled thereto, may be reverse transcribed into complementary cDNA. The cDNA may be sequenced. The donor vector or cDNA may be sequenced using amplicon sequencing. The donor vector sequence and the cDNA sequence may be correlated to identify the variant and the cell's expression of the variant.

公开(公告)号：US20210238589A1

公开(公告)日：2021-08-05

申请号：US17166906

申请日：2021-02-03

申请人： ILLUMINA, INC.

发明人： Dan Cao ,  Jeffrey S. Fisher ,  Fiona Kaper ,  Tarun Kumar Khurana ,  Tong Liu ,  Burak Okumus ,  Victor J. Quijano ,  Clifford Lee Wang ,  Yir-Shyuan Wu ,  Shi Min Xiao ,  Hongxia Xu

IPC分类号： C12N15/10

摘要： An example of a biotin-streptavidin cleavage composition includes a formamide reagent and a salt buffer. The formamide reagent is present in the biotin-streptavidin cleavage composition in an amount ranging from about 10% to about 50%, based on a total volume of the biotin-streptavidin cleavage composition. The salt buffer makes up the balance of the biotin-streptavidin cleavage composition. In some examples, the biotin-streptavidin cleavage composition is used to cleave library fragments from a solid support. In other examples, other mechanisms are used to cleave library fragments from a solid support.

公开(公告)号：US20180291444A1

公开(公告)日：2018-10-11

申请号：US15935897

申请日：2018-03-26

申请人： ILLUMINA, INC.

发明人： Hongxia Xu ,  Alex Aravanis ,  Shengrong Lin

IPC分类号： C12Q1/6874 ,  C12Q1/6837 ,  C12N15/10

摘要： The present disclosure relates to the field of molecular biology and more specifically to methods for capturing, amplifying and sequencing target polynucleotides on a solid surface.