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1.
公开(公告)号:US20220331770A1
公开(公告)日:2022-10-20
申请号:US17659037
申请日:2022-04-13
发明人: Sébastien RICOULT , Pascale MATHONET , Elliot LAWRENCE , Niall GORMLEY , Justin DAVIDSON , Kim SCHNEIDER , Antoine FRANCAIS , Jessica WALSH , Johan Sebastian BASUKI , Shima GHOLIZADEH
IPC分类号: B01J13/04 , C12Q1/6876
摘要: The present disclosure relates to a composition comprising a shell surrounding a core, wherein the core comprises one or more lyophilised microspheres. Also described herein is a method comprising providing one or more lyophilised microspheres; and coating the one or more lyophilised microspheres with a shell under conditions effective to encapsulate the one or more lyophilised microspheres. The present disclosure further relates to a system comprising one or more composition as described herein, and one or more lyophilised cake, wherein the one or more composition and the one or more lyophilised cake are combined under conditions effective to form a rehydration system. Also described herein is a method of controlling release of one or more encapsulated microspheres comprising providing a composition as described herein and mixing the composition with a rehydration solution under a first condition effective to control release of one or more lyophilised microspheres from the composition.
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公开(公告)号:US20230279385A1
公开(公告)日:2023-09-07
申请号:US18169970
申请日:2023-02-16
发明人: Frank J. STEEMERS , Jonathan Mark BOUTELL , Pietro GATTI LAFRANCONI , Oliver Jon MILLER , Emma BELL , Sebastien Georg Gabriel RICOULT , Niall Anthony GORMLEY , Kim SCHNEIDER
CPC分类号: C12N15/1065 , C12N15/11 , C12N9/22 , C12N9/1241 , C12N2310/20 , C12N2800/80 , C12N2800/90
摘要: A variety of different types of targeted transposome complexes are described herein that may be used to mediate sequence-specific targeted transposition of nucleic acids. Also described herein is a method of characterizing desired samples in a mixed pool of samples comprising both desired samples and unwanted samples comprising, to produce sequencing data from double-stranded nucleic acid, initially sequencing a library comprising a plurality of nucleic acid samples from a mixed pool, wherein each nucleic acid library comprises nucleic acids from a single sample and a unique sample barcode to distinguish the nucleic acids from the single sample from the nucleic acids from other samples in the library; analyzing the sequencing data and identifying unique sample barcodes associated with sequencing data from desired samples; performing a selection step on the library comprising enriching nucleic acid samples from desired samples and/or depleting nucleic acid samples from unwanted samples; and resequencing the nucleic acid library.
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