公开(公告)号：US11885800B2

公开(公告)日：2024-01-30

申请号：US17037162

申请日：2020-09-29

Applicant: IMRA America, Inc.

Inventor： Bing Liu ,  Matthew L. Elani ,  Alison R. Garrett

IPC: G01N33/543 ,  G01N27/74 ,  G01N33/68 ,  G01N33/76 ,  G01N35/00

CPC classification number: G01N33/54333 ,  G01N27/745 ,  G01N33/54326 ,  G01N33/6887 ,  G01N33/76 ,  B01L2200/025 ,  G01N35/0098

Abstract: A system includes an apparatus having at least one permanent magnet and at least one magnetic field sensor at a pole of the at least one permanent magnet and configured to be positioned relative to a surface of a membrane containing immobilized magnetic particles selectively bound to an analyte such that the magnetic particles are magnetized by the at least one permanent magnet. The system further includes a stage configured to move at least one of the apparatus and the membrane relative to one another with an oscillatory movement parallel to the surface of the membrane, at least one controller configured to control the oscillatory movement, and a data acquisition unit configured to receive signals from the at least one magnetic field sensor and the at least one controller method.

公开(公告)号：US20220178919A1

公开(公告)日：2022-06-09

申请号：US17037162

申请日：2020-09-29

Applicant: IMRA America, Inc.

Inventor： Bing Liu ,  Matthew L. Elani ,  Alison R. Garrett

IPC: G01N33/543 ,  G01N33/76 ,  G01N33/68 ,  G01N27/74

Abstract: A method, system, and apparatus for the rapid detection of analyte(s) of interest are disclosed which can provide high sensitivity quantification of the analyte concentration in a lateral follow assay. The method includes labeling detection molecules with magnetic particles and immobilizing the magnetic particles on a nitrocellulose membrane upon specific biochemical recognition and binding. An external magnetic field is applied to the magnetic particles to induce magnetic induction, and a magnetoresistance sensor is positioned close to the membrane and magnetic particles. A periodic signal in the sensor is produced when a mechanical oscillatory movement is provided to the membrane relative to the sensor (or vice versa). Triggered time averaging of signals in synchronization with the oscillatory motion enables noise reduction of less than 30 dB and significant improvement of assay sensitivity. An x-y motion program for scanning the test line and control line on the membrane can produce magnetic 2D mapping of the lines, further differentiating the bound particles at the lines from unbound particles in the background, rendering a more accurate assay.