-
公开(公告)号:US5310659A
公开(公告)日:1994-05-10
申请号:US779049
申请日:1991-10-18
申请人: Ichiro Aramori , Masao Fukagawa , Hiroki Ono , Yosuke Ishitani , Mana Tsumura , Morita Iwami , Hitoshi Kojo
发明人: Ichiro Aramori , Masao Fukagawa , Hiroki Ono , Yosuke Ishitani , Mana Tsumura , Morita Iwami , Hitoshi Kojo
摘要: A GL-7ACA acylase having the following characteristics:(a) has ability to catalyze the enzymatic conversion of glutaryl 7-ACA, adipyl 7-ACA, and succinyl 7-ACA, into 7-aminocephalosporanic acid,(b) has a molecular weight of 70,000 dalton (SDS-PAGE) and(c) has N-terminal amino acid sequence (SEQ ID NO: 1) thereof: Gln-Ser-Glu-Gln-Glu-Lys-Ala-Glu-Glu-.A process for producing GL-7ACA acylase is also provided.
摘要翻译: 具有以下特征的GL-7ACA酰基转移酶:(a)具有催化戊二酰7-ACA,己二酰基7-ACA和琥珀酰基7-ACA酶促转化成7-氨基头孢烷酸的能力,(b)具有分子量 的70,000道尔顿(SDS-PAGE)和(c)具有N-末端氨基酸序列(SEQ ID NO:1):Gln-Ser-Glu-Gln-Glu-Lys-Ala-Glu-Glu-。 还提供了生产GL-7ACA酰基转移酶的方法。
-
公开(公告)号:US5312750A
公开(公告)日:1994-05-17
申请号:US80240
申请日:1993-06-22
申请人: Ichiro Aramori , Masao Fukagawa , Hiroki Ono , Yosuke Ishitani , Mana Tsumura , Morita Iwami , Hitoshi Kojo
发明人: Ichiro Aramori , Masao Fukagawa , Hiroki Ono , Yosuke Ishitani , Mana Tsumura , Morita Iwami , Hitoshi Kojo
IPC分类号: C12N1/21 , C12N9/80 , C12N15/09 , C12N15/55 , C12P35/02 , C12R1/07 , C12R1/19 , C12D21/06 , C12N15/00
摘要: A GL-7ACA acylase having the following characteristics:(a) has ability to catalyze the enzymatic conversion of glutaryl 7-ACA, adipyl 7-ACA, and succinyl 7-ACA, into 7-aminocephalosporanic acid,(b) has a molecular weight of 70,000 dalton (SDS-PAGE) and(c) has N-terminal amino acid sequence (sea in No: 1) thereof: Gln-Ser-Glu-Gln-Glu-Lys-Ala-Glu-Glu-.A process for producing GL-7ACA acylase is also provided.
摘要翻译: 具有以下特征的GL-7ACA酰基转移酶:(a)具有催化戊二酰7-ACA,己二酰基7-ACA和琥珀酰基7-ACA酶促转化成7-氨基头孢烷酸的能力,(b)具有分子量 的70,000道尔顿(SDS-PAGE)和(c)具有N-末端氨基酸序列(海洋号:1):Gln-Ser-Glu-Gln-Glu-Lys-Ala-Glu-Glu-。 还提供了生产GL-7ACA酰基转移酶的方法。
-