公开(公告)号：US11725193B2

公开(公告)日：2023-08-15

申请号：US16619637

申请日：2018-05-25

申请人： IKEDA FOOD RESEARCH CO., LTD.

发明人： Michinari Honda ,  Hirokazu Sanada ,  Ryo Takenaka ,  Takafumi Takumi ,  Takahiro Fujii

IPC分类号： C12N9/04 ,  C12N15/70 ,  C12Q1/32

CPC分类号： C12N9/0006 ,  C12N15/70 ,  C12Q1/32

摘要： [Problem]

To provide a glucose dehydrogenase with high thermal stability to be used in blood sugar measurement.


[Solution to Problem to problem]

A modified glucose dehydrogenase with an excellent property of high thermal stability that can be obtained by modifying some of amino acids constituting a protein such as a wild-type glucose dehydrogenase, a polynucleotide encoding the enzyme, a method for manufacturing the enzyme, a method for measuring glucose using the enzyme, a measuring reagent composition and a biosensor, and methods for manufacturing the measuring reagent composition and the biosensor.

公开(公告)号：US20150152394A1

公开(公告)日：2015-06-04

申请号：US14389495

申请日：2013-03-29

申请人： IKEDA FOOD RESEARCH CO., LTD.

发明人： Michinari Honda ,  Ryo Takenaka ,  Takafumi Takumi

IPC分类号： C12N9/04 ,  C12Q1/32 ,  G01N27/327 ,  C12Q1/54

CPC分类号： C12N9/0006 ,  C12Q1/32 ,  C12Q1/54 ,  C12Y101/9901 ,  G01N27/327

摘要： The purpose of the invention is to provide flavin-conjugated glucose dehydrogenase having little variation in activity in the typical biosensor measurement temperature range (10-40° C.) and a method for measuring glucose using same. The present invention relates to flavin-conjugated glucose dehydrogenase having the following properties (1)-(3) and the like: (1) action: shows glucose dehydrogenase activity in the presence of an electron acceptor; (2) substrate specificity: the activity value is 10% or less relative to maltose, D-galactose, D-fructose, sorbitol, lactose, and sucrose when the activity value relative to D-glucose is taken to be 100%; and (3) temperature characteristics: the range of the activity value at 10-40° C. is 20-150% when the activity value at 30° C. is taken to be 100%.

摘要翻译： 本发明的目的是提供在典型的生物传感器测量温度范围（10-40℃）中几乎没有活性变化的黄素缀合的葡萄糖脱氢酶和使用其测量葡萄糖的方法。 本发明涉及具有以下性质（1） - （3）等的黄素缀合的葡萄糖脱氢酶：（1）作用：在电子受体存在下显示葡萄糖脱氢酶活性; （2）底物特异性：当相对于D-葡萄糖的活性值取为100％时，相对于麦芽糖，D-半乳糖，D-果糖，山梨醇，乳糖和蔗糖，活性值为10％以下。 和（3）温度特性：当将30℃下的活性值取为100％时，10-40℃下的活性值的范围为20-150％。

公开(公告)号：US09663811B2

公开(公告)日：2017-05-30

申请号：US15135375

申请日：2016-04-21

申请人： Ikeda Food Research Co., Ltd.

发明人： Takako Yada ,  Koji Miyamoto ,  Michinari Honda

IPC分类号： C12Q1/00 ,  C12Q1/32 ,  G01N27/30 ,  G01N33/66 ,  G01N27/327 ,  C12N9/04 ,  C12Q1/54

CPC分类号： C12N9/0006 ,  C12Q1/006 ,  C12Q1/32 ,  C12Q1/54 ,  C12Y101/9901 ,  G01N27/301 ,  G01N27/302 ,  G01N27/3271 ,  G01N33/66 ,  G01N2333/902 ,  G01N2333/904 ,  Y10T29/49117

摘要： An object of the present invention is to provide: a novel gene (polynucleotide) encoding an FAD-conjugated glucose dehydrogenase having excellent properties that it has excellent reactivity to glucose, excellent thermal stability, and excellent substrate-recognition performance and also has a low activity for maltose; a process for the production of the enzyme using a transformant cell transfected with the gene; and a method for the determination of glucose, a reagent composition for use in the determination of glucose, a biosensor for use in the determination of glucose and others, each characterized by using the enzyme obtained. The invention relates to a polynucleotide encoding an FAD-conjugated glucose dehydrogenase, comprising a polypeptide containing an amino acid sequence: X1-X2-X3-X4-X5-X6 (wherein X1 and X2 independently represent an aliphatic amino acid residue; X3 and X6 independently represent a branched amino acid residue; and X4 and X5 independently represent a heterocyclic amino acid residue or an aromatic amino acid residue); and others.

公开(公告)号：US09340816B2

公开(公告)日：2016-05-17

申请号：US14510076

申请日：2014-10-08

申请人： Ikeda Food Research Co., Ltd.

发明人： Takako Yada ,  Koji Miyamoto ,  Michinari Honda

IPC分类号： C12Q1/00 ,  C12Q1/54 ,  C12N9/04 ,  C12Q1/32 ,  G01N33/66

CPC分类号： C12N9/0006 ,  C12Q1/006 ,  C12Q1/32 ,  C12Q1/54 ,  C12Y101/9901 ,  G01N27/301 ,  G01N27/302 ,  G01N27/3271 ,  G01N33/66 ,  G01N2333/902 ,  G01N2333/904 ,  Y10T29/49117

摘要： Disclosed herein are a gene (polynucleotide) encoding an FAD-conjugated glucose dehydrogenase which can be characterized by reactivity to glucose, thermal stability, substrate-recognition performance, and low activity for maltose; a process for the production of the enzyme using a transformant cell transfected with the gene; a method for the determination of glucose; a reagent composition for use in the determination of glucose; and a biosensor for use in the determination of glucose. An embodiment is a polynucleotide encoding an FAD-conjugated glucose dehydrogenase, comprising a polypeptide whose amino acid sequence comprises X1-X2-X3-X4-X5-X6, wherein X1 and X2 independently represent an aliphatic amino acid residue; X3 and X6 independently represent a branched amino acid residue; and X4 and X5 independently represent a heterocyclic amino acid residue or an aromatic amino acid residue.

摘要翻译： 本文公开了编码FAD-缀合的葡萄糖脱氢酶的基因（多核苷酸），其可以表征为对葡萄糖的反应性，热稳定性，底物识别性能和对麦芽糖的低活性; 使用用该基因转染的转化体细胞来生产酶的方法; 一种测定葡萄糖的方法; 用于测定葡萄糖的试剂组合物; 以及用于测定葡萄糖的生物传感器。 一个实施方案是编码FAD-缀合的葡萄糖脱氢酶的多核苷酸，其包含其氨基酸序列包含X 1 -X 2 -X 3 -X 4 -X 5 -X 6的多肽，其中X 1和X 2独立地表示脂族氨基酸残基; X3和X6独立地表示支链氨基酸残基; X4和X5独立地表示杂环氨基酸残基或芳香族氨基酸残基。

公开(公告)号：US08882978B2

公开(公告)日：2014-11-11

申请号：US13920445

申请日：2013-06-18

申请人： Ikeda Food Research Co., Ltd

发明人： Takako Yada ,  Koji Miyamoto ,  Michinari Honda

IPC分类号： C12M1/00 ,  C12Q1/54 ,  C12N9/00 ,  C12N9/96 ,  C12N1/20 ,  C12N15/00 ,  G01N33/66 ,  C12Q1/32 ,  C12Q1/00 ,  C12N9/04

CPC分类号： C12N9/0006 ,  C12Q1/006 ,  C12Q1/32 ,  C12Q1/54 ,  C12Y101/9901 ,  G01N27/301 ,  G01N27/302 ,  G01N27/3271 ,  G01N33/66 ,  G01N2333/902 ,  G01N2333/904 ,  Y10T29/49117

摘要： Disclosed herein are a gene (polynucleotide) encoding an FAD-conjugated glucose dehydrogenase which can be characterized by reactivity to glucose, thermal stability, substrate-recognition performance, and low activity for maltose; a process for the production of the enzyme using a transformant cell transfected with the gene; a method for the determination of glucose; a reagent composition for use in the determination of glucose; and a biosensor for use in the determination of glucose. An embodiment is a polynucleotide encoding an FAD-conjugated glucose dehydrogenase, comprising a polypeptide whose amino acid sequence comprises X1-X2-X3-X4-X5-X6, wherein X1 and X2 independently represent an aliphatic amino acid residue; X3 and X6 independently represent a branched amino acid residue; and X4 and X5 independently represent a heterocyclic amino acid residue or an aromatic amino acid residue.

摘要翻译： 本文公开了编码FAD-缀合的葡萄糖脱氢酶的基因（多核苷酸），其可以表征为对葡萄糖的反应性，热稳定性，底物识别性能和对麦芽糖的低活性; 使用用该基因转染的转化体细胞来生产酶的方法; 一种测定葡萄糖的方法; 用于测定葡萄糖的试剂组合物; 以及用于测定葡萄糖的生物传感器。 一个实施方案是编码FAD-缀合的葡萄糖脱氢酶的多核苷酸，其包含其氨基酸序列包含X 1 -X 2 -X 3 -X 4 -X 5 -X 6的多肽，其中X 1和X 2独立地表示脂族氨基酸残基; X3和X6独立地表示支链氨基酸残基; X4和X5独立地表示杂环氨基酸残基或芳香族氨基酸残基。

公开(公告)号：US09976125B2

公开(公告)日：2018-05-22

申请号：US15496935

申请日：2017-04-25

申请人： Ikeda Food Research Co., Ltd.

发明人： Takako Yada ,  Koji Miyamoto ,  Michinari Honda

IPC分类号： C12N9/04 ,  C12Q1/00 ,  G01N27/30 ,  G01N27/327 ,  G01N33/66 ,  C12Q1/54 ,  C12Q1/32

CPC分类号： C12N9/0006 ,  C12Q1/006 ,  C12Q1/32 ,  C12Q1/54 ,  C12Y101/9901 ,  G01N27/301 ,  G01N27/302 ,  G01N27/3271 ,  G01N33/66 ,  G01N2333/902 ,  G01N2333/904 ,  Y10T29/49117

摘要： An object of the present invention is to provide: a novel gene (polynucleotide) encoding an FAD-conjugated glucose dehydrogenase having excellent properties that it has excellent reactivity to glucose, excellent thermal stability, and excellent substrate-recognition performance and also has a low activity for maltose; a process for the production of the enzyme using a transformant cell transfected with the gene; and a method for the determination of glucose, a reagent composition for use in the determination of glucose, a biosensor for use in the determination of glucose and others, each characterized by using the enzyme obtained. The invention relates to a polynucleotide encoding an FAD-conjugated glucose dehydrogenase, comprising a polypeptide containing an amino acid sequence: X1-X2-X3-X4-X5-X6 (wherein X1 and X2 independently represent an aliphatic amino acid residue; X3 and X6 independently represent a branched amino acid residue; and X4 and X5 independently represent a heterocyclic amino acid residue or an aromatic amino acid residue); and others.

公开(公告)号：US08945359B2

公开(公告)日：2015-02-03

申请号：US13777827

申请日：2013-02-26

申请人： Ikeda Food Research Co., Ltd.

发明人： Michinari Honda ,  Sayaka Taki ,  Ryo Takenaka

IPC分类号： C12N9/04 ,  C12Q1/32 ,  G01N27/327 ,  C12Q1/00

CPC分类号： C12N9/0006 ,  C12Q1/006 ,  C12Q1/32 ,  C12Y101/01047 ,  C12Y101/9901

摘要： The invention provides a flavin-binding glucose dehydrogenase exhibiting reduced fluctuation of activity depending on temperature environment, and a method for measuring glucose concentration using the flavin-binding glucose dehydrogenase. The flavin-binding glucose dehydrogenase has the following properties (1) to (3): (1) activity: which exhibits glucose dehydrogenase activity in the presence of an electron acceptor; (2) substrate specificity: which exhibits an activity of 10% or less against maltose, D-galactose, D-fructose, sorbitol, lactose and sucrose when the activity against D-glucose is defined as 100%; and (3) temperature characteristics: which exhibits lower fluctuation of activity in a wide temperature range of 10 to 50° C.

摘要翻译： 本发明提供了一种黄色素结合性葡萄糖脱氢酶，其显示出根据温度环境降低的活性波动，以及使用黄素结合葡萄糖脱氢酶测量葡萄糖浓度的方法。 黄素结合葡萄糖脱氢酶具有以下性质（1）至（3）：（1）活性：在电子受体存在下显示葡萄糖脱氢酶活性; （2）底物特异性：当对D-葡萄糖的活性定义为100％时，其对麦芽糖，D-半乳糖，D-果糖，山梨糖醇，乳糖和蔗糖具有10％或更低的活性; 和（3）温度特性：在10〜50℃的较宽的温度范围内表现出较低的活性波动。