公开(公告)号：US20190009273A1

公开(公告)日：2019-01-10

申请号：US16105002

申请日：2018-08-20

Applicant: Indiana University Research and Technology Corporation

Inventor： James A. GLAZIER ,  Stephen C. JACOBSON ,  Dragos AMARIE

IPC: B01L3/00 ,  B01F5/06 ,  B01F3/08 ,  B01F13/00 ,  B01F15/04

CPC classification number: B01L3/502715 ,  B01F3/0865 ,  B01F5/06 ,  B01F5/0601 ,  B01F13/0059 ,  B01F13/0062 ,  B01F15/0404 ,  B01F2003/0896 ,  B01F2215/0037 ,  B01L3/502776 ,  B01L2200/0694 ,  B01L2200/12 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0896 ,  B01L2400/0415

Abstract: Disclosed is a method and apparatus for manipulating fluids. The apparatus may include a microfluidic structure including inlet channels (1 and 2) and outlet channels (306, 307, 308, 309, 310, 311, 312, 313, and 314) oriented among bifurcated (5), trifurcated (6) and merging junctions (7 and 8). The apparatus splits and merges fluids flowing in the channels to produce successive dilutions of the fluids within the outlet channels. Multiple apparatus may be combined in serial, parallel, combined serial and parallel and/or stacked configurations. One or more apparatus may be used alone or to provide various devices or chambers with the diluted fluids.

公开(公告)号：US20160310945A1

公开(公告)日：2016-10-27

申请号：US15198359

申请日：2016-06-30

Applicant: Indiana University Research and Technology Corporation

Inventor： James A. GLAZIER ,  Stephen C. JACOBSON ,  Dragos AMARIE

IPC: B01L3/00 ,  B01F3/08 ,  B01F15/04 ,  B01F13/00

CPC classification number: B01L3/502715 ,  B01F3/0865 ,  B01F5/06 ,  B01F5/0601 ,  B01F13/0059 ,  B01F13/0062 ,  B01F15/0404 ,  B01F2003/0896 ,  B01F2215/0037 ,  B01L3/502776 ,  B01L2200/0694 ,  B01L2200/12 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0896 ,  B01L2400/0415

Abstract: Disclosed is a method and apparatus for manipulating fluids. The apparatus may include a microfluidic structure including inlet channels (1 and 2) and outlet channels (306, 307, 308, 309, 310, 311, 312, 313, and 314) oriented among bifurcated (5), trifurcated (6) and merging junctions (7 and 8). The apparatus splits and merges fluids flowing in the channels to produce successive dilutions of the fluids within the outlet channels. Multiple apparatus may be combined in serial, parallel, combined serial and parallel and/or stacked configurations. One or more apparatus may be used alone or to provide various devices or chambers with the diluted fluids.

Abstract translation: 公开了一种用于操纵流体的方法和装置。 该装置可以包括微流体结构，其包括取向于分叉（5），三叉（6）和（3）的入口通道（1和2）和出口通道（306,307,308,309,310,311,312,313和314） 合并路口（7和8）。 该装置分裂和合并在通道中流动的流体以在出口通道内产生连续稀释的流体。 多个装置可以串联，并联，组合的串行和并行和/或堆叠配置组合。 一个或多个装置可以单独使用或者为稀释流体提供各种装置或室。

公开(公告)号：US20160168523A1

公开(公告)日：2016-06-16

申请号：US15050356

申请日：2016-02-22

Applicant: Indiana University Research and Technology Corporation

Inventor： James A. GLAZIER ,  Abdelkrim ALILECHE ,  Abbas SHIRINIFARD ,  Dragos AMARIE

IPC: C12M3/00 ,  C12N5/00 ,  A61K35/44 ,  C12M1/00

CPC classification number: C12M21/08 ,  A61K35/44 ,  C12M23/10 ,  C12M23/20 ,  C12N5/0068 ,  C12N2533/90

Abstract: Disclosed herein are capillary fabrication devices comprising living cells within a support medium. Culture of the cells produces viable lumenized capillary networks with natural or pre-determined geometries and ECM and basement membrane associated with the capillary networks. The capillary networks and the ECM and basement membrane detachable from the capillary networks are useful for tissue engineering applications.

Abstract translation: 本文公开了包括支撑介质内的活细胞的毛细管制造装置。 细胞的培养产生具有天然或预定几何形状的可行的管腔毛细血管网络，以及与毛细管网络相关联的ECM和基底膜。 毛细管网络和可从毛细管网络拆卸的ECM和基底膜可用于组织工程应用。