公开(公告)号：US12054789B2

公开(公告)日：2024-08-06

申请号：US16792734

申请日：2020-02-17

申请人： Iowa State University Research Foundation, Inc.

发明人： Jared L. Anderson ,  Byron F. Brehm-Stecher ,  Kevin D. Clark ,  Stephanie A. Hice

IPC分类号： C12Q1/68 ,  C07F5/00 ,  C07F13/00 ,  C07F15/04 ,  C07F15/06 ,  C12N1/02 ,  C12N1/20 ,  C12N15/10 ,  C12Q1/04 ,  C12Q1/24 ,  C12Q1/6806 ,  C12Q1/689

CPC分类号： C12Q1/689 ,  C07F5/003 ,  C07F13/005 ,  C07F15/045 ,  C07F15/065 ,  C12N1/02 ,  C12N1/20 ,  C12N15/1013 ,  C12Q1/04 ,  C12Q1/24 ,  C12Q1/6806

摘要： In this disclosure, a method or kit for using the method of extracting, concentrating, and detecting microbes from a sample is disclosed. The method disclosed herein use transition or rare earth metal-based magnetic ionic liquids (MILs) to extract viable microbes from a sample and to detect them via an amplification-based method and/or a non-amplification-based methods. The method and kit can be used in-field and on-site for detection of viable microbes in a sample within about an hour, without using any powered heat source or powered tool.

公开(公告)号：US20240336982A1

公开(公告)日：2024-10-10

申请号：US18752431

申请日：2024-06-24

申请人： Iowa State University Research Foundation, Inc.

发明人： Jared L. Anderson ,  Byron F. Brehm-Stecher ,  Kevin D. Clark ,  STEPHANIE A. HICE

IPC分类号： C12Q1/689 ,  C07F5/00 ,  C07F13/00 ,  C07F15/04 ,  C07F15/06 ,  C12N1/02 ,  C12N1/20 ,  C12N15/10 ,  C12Q1/04 ,  C12Q1/24 ,  C12Q1/6806

CPC分类号： C12Q1/689 ,  C07F5/003 ,  C07F13/005 ,  C07F15/045 ,  C07F15/065 ,  C12N1/02 ,  C12N1/20 ,  C12N15/1013 ,  C12Q1/04 ,  C12Q1/24 ,  C12Q1/6806

摘要： In this disclosure, a method or kit for using the method of extracting, concentrating, and detecting microbes from a sample is disclosed. The method disclosed herein use transition or rare earth metal-based magnetic ionic liquids (MILs) to extract viable microbes from a sample and to detect them via an amplification-based method and/or a non-amplification-based methods. The method and kit can be used in-field and on-site for detection of viable microbes in a sample within about an hour, without using any powered heat source or powered tool.

公开(公告)号：US20200325525A1

公开(公告)日：2020-10-15

申请号：US16792734

申请日：2020-02-17

申请人： Iowa State University Research Foundation, Inc.

发明人： Jared L. Anderson ,  Byron F. Brehm-Stecher ,  Kevin D. Clark ,  Stephanie A. Hice

IPC分类号： C12Q1/689 ,  C12N1/02 ,  C07F13/00 ,  C07F15/06 ,  C07F15/04 ,  C07F5/00

摘要： In this disclosure, a method or kit for using the method of extracting, concentrating, and detecting microbes from a sample is disclosed. The method disclosed herein use transition or rare earth metal-based magnetic ionic liquids (MILs) to extract viable microbes from a sample and to detect them via an amplification-based method and/or a non-amplification-based methods. The method and kit can be used in-field and on-site for detection of viable microbes in a sample within about an hour, without using any powered heat source or powered tool.