公开(公告)号：US20240158767A1

公开(公告)日：2024-05-16

申请号：US18416306

申请日：2024-01-18

申请人： IsoPlexis Corporation

发明人： Jerzy OLEJNIK ,  Angela DELUCIA ,  Ralf PEIST ,  Nicole GRASSE

IPC分类号： C12N9/12 ,  C12N15/63 ,  C12N15/90

CPC分类号： C12N9/1252 ,  C12N15/63 ,  C12N15/90

摘要： The present invention relates to a polymerase enzyme with improved ability to incorporate reversibly terminating nucleotides. The enzyme comprising the following mutations in the motif A region (SGS). It relates to a polymerase enzyme according to SEQ ID NO. 1 or any polymerase that shares at least 70% amino acid sequence identity thereto, comprising a mutation selected from the group of (i) at position 412 of SEQ ID NO. 1: serine (S) (L412S) and/or, (ii) at position 413 of SEQ ID NO. 1: glycine (G) (Y413G) and/or (iii) at position 414 of SEQ ID NO. 1: serine (S) (P414S), wherein the enzyme has little or no 3′-5′ exonuclease activity.

公开(公告)号：US20240076731A1

公开(公告)日：2024-03-07

申请号：US18178159

申请日：2023-03-03

申请人： IsoPlexis Corporation

发明人： Steven GORDON ,  Jerzy OLEJNIK

IPC分类号： C12Q1/6869 ,  C07H19/00 ,  C12Q1/6874 ,  G01N21/05 ,  G01N21/64

CPC分类号： C12Q1/6869 ,  C07H19/00 ,  C12Q1/6874 ,  G01N21/05 ,  G01N21/6428 ,  G01N21/645 ,  C12Q2535/101 ,  G01N2021/0346 ,  G01N2021/058 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2021/6471

摘要： The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.

公开(公告)号：US20210355529A1

公开(公告)日：2021-11-18

申请号：US17386883

申请日：2021-07-28

申请人： IsoPlexis Corporation

发明人： Jerzy OLEJNIK ,  Michel Georges PERBOST

IPC分类号： C12Q1/6823 ,  C12Q1/6869

摘要： The invention relates to methods, compositions, devices, systems and kits as described including, without limitation, reagents and mixtures for determining the identity of nucleic acids in nucleotide sequences using, for example, sequencing by synthesis methods. In particular, the present invention contemplates the use of polyphenolic compounds, known as antioxidant additives, to improve the efficiency of Sequencing-By-Synthesis reactions. For example, gallic acid (GA) is shown herein to be one of many exemplary SBS polyphenolic additives.

公开(公告)号：US20240093164A1

公开(公告)日：2024-03-21

申请号：US18519620

申请日：2023-11-27

申请人： IsoPlexis Corporation

发明人： Jerzy OLEJNIK ,  Angela DELUCIA

IPC分类号： C12N9/12 ,  C12Q1/6844 ,  C12Q1/6869

CPC分类号： C12N9/1252 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Y207/07007

摘要： The present invention relates to a polymerase enzyme from 9° N with improved ability to incorporate reversibly terminating nucleotides. The enzyme comprising mutations in the motif A region. The invention also relates to methods of using such enzymes as well as a kit with such polymerases.

公开(公告)号：US20240060128A1

公开(公告)日：2024-02-22

申请号：US18361434

申请日：2023-07-28

申请人： IsoPlexis Corporation

发明人： Steven GORDON ,  Jerzy OLEJNIK

IPC分类号： C12Q1/6869 ,  C07H19/00 ,  C12Q1/6874 ,  G01N21/05 ,  G01N21/64

CPC分类号： C12Q1/6869 ,  C07H19/00 ,  C12Q1/6874 ,  G01N21/05 ,  G01N21/6428 ,  G01N21/645 ,  C12Q2535/101 ,  G01N2021/0346 ,  G01N2021/058 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2021/6471

摘要： The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.

公开(公告)号：US20230407384A1

公开(公告)日：2023-12-21

申请号：US18325668

申请日：2023-05-30

申请人： IsoPlexis Corporation

发明人： Steven GORDON ,  Jerzy OLEJNIK

IPC分类号： C12Q1/6869 ,  G01N21/64 ,  C12Q1/6874 ,  C07H19/00 ,  G01N21/05 ,  G01N21/03

CPC分类号： C12Q1/6869 ,  G01N21/6428 ,  C12Q1/6874 ,  G01N21/645 ,  C07H19/00 ,  G01N21/05 ,  G01N2201/062 ,  G01N2201/0627 ,  G01N2021/058 ,  G01N2021/6421 ,  G01N2021/0346 ,  G01N2201/12 ,  G01N2021/6417 ,  G01N2021/6471 ,  C12Q2535/101 ,  G01N2021/6441 ,  G01N2021/6439 ,  G01N2021/6419

摘要： The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.