公开(公告)号：US20230025239A1

公开(公告)日：2023-01-26

申请号：US17781823

申请日：2019-12-02

申请人： JILIN ASYMCHEM LABORATORIES CO., LTD.

发明人： Hao HONG ,  Gage JAMES ,  Vyasarayani Williams RAJASEKAR ,  Yuxia CUI ,  Na ZHANG ,  Jiadong ZHAO ,  Mingmin HAO ,  Yanyan GAO

IPC分类号： C12N11/18 ,  C12N11/089

摘要： Provided are a co-immobilized enzyme, a preparation method and use thereof. The co-immobilized enzyme includes: an amino resin carrier, a main enzyme, and a coenzyme. The main enzyme and the coenzyme are co-immobilized on the amino resin carrier, herein the main enzyme is covalent-immobilized on the amino resin carrier, and the coenzyme is immobilized on the amino resin carrier by a mode of covalent and/or non-covalent; and the main enzyme is selected from any one of the following enzymes: transaminase, amino acid dehydrogenase, imine reductase, ketoreductase, enoyl reductase, and monooxygenase. The main enzyme and the coenzyme thereof are co-immobilized on the amino resin carrier for co-immobilization, so the activity and the recycling efficiency of the enzyme are improved.

公开(公告)号：US20230295604A1

公开(公告)日：2023-09-21

申请号：US17789235

申请日：2019-12-25

申请人： JILIN ASYMCHEM LABORATORIES CO., LTD.

发明人： Hao HONG ,  Gage JAMES ,  Yi XIAO ,  Na ZHANG ,  Vyasarayani Williams RAJASEKAR ,  Yuxia CUI ,  Jiadong ZHAO ,  Mingmin HAO ,  Yanyan GAO

IPC分类号： C12N11/091 ,  C12N9/10 ,  C12N9/04 ,  C12N9/02 ,  C12N9/88 ,  C12N9/06 ,  C12N11/06

CPC分类号： C12N11/091 ,  C12N9/0006 ,  C12N9/0016 ,  C12N9/0073 ,  C12N9/88 ,  C12N9/1096 ,  C12N11/06 ,  C12Y104/01009 ,  C12Y114/13022 ,  C12Y403/01024

摘要： Provided is an immobilized enzyme, a preparation method and use thereof. The immobilized enzyme includes an enzyme and an amino resin carrier for immobilizing the enzyme, and the enzyme is selected from any one of the following enzymes: transaminase, ketoreductase, monooxygenase, ammonia-lyase, ene reductase, imine reductase, amino acid dehydrogenase, and nitrilase. The amino resin carrier is an amino resin carrier modified by a cross-linking agent, and the cross-linking agent is a cross-linking agent treated by a polymer. By means of modifying the amino resin carrier with the cross-linking agent treated by the polymer, the enzyme immobilized on the amino resin carrier may easily form a network cross-linking, such that the immobilization effect of the enzyme is more stable, thereby the recycling efficiency of the enzyme is improved.