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1.发明授权Use of tryptophan indole and anthranilate analogs as plant transformation selection agents 有权使用色氨酸吲哚和邻氨基苯甲酸酯类似物作为植物转化选择剂公开(公告)号：US07847152B2
公开(公告)日：2010-12-07
申请号：US11770030
申请日：2007-06-28
CPC分类号： C12N15/8209 , C12N9/88 , C12N15/821 , C12N15/8214 , C12N15/8254
摘要： Methods and compositions are described using tryptophan analogs, indole analogs, and mixtures thereof as agents for selecting plant cells that have been transformed with an anthranilate synthase (ASA2) gene or a fragment thereof, where the ASA2 gene encodes a feedback-insensitive form of anthranilate synthase (AS).
摘要翻译： 使用色氨酸类似物，吲哚类似物及其混合物描述了用于选择已经用邻氨基苯甲酸合酶（ASA2）基因或其片段转化的植物细胞的试剂的方法和组合物，其中ASA2基因编码反相不敏感形式的邻氨基苯甲酸 合成酶（AS）。
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2.发明授权In vitro screening for and selection of glycine max resistant to phialophora gregata 失效体外筛选和选择对phialophora gregata有抗性的甘氨酸
公开(公告)号：US4937970A
公开(公告)日：1990-07-03
申请号：US778384
申请日：1985-09-20
申请人： Yong-Quan Guan , Lynn E. Gray , Jack M. Widholm
发明人： Yong-Quan Guan , Lynn E. Gray , Jack M. Widholm
CPC分类号： A01H1/04 , C12Q1/6895 , Y10S47/01
摘要： An in vitro method for screening Glycine max cells for resistance to a disease caused by Phialophora gregata, brown stem rot, is disclosed. The method is useful for identifying brown stem rot resistant soybean varieties. Means for selecting mutant soybean cells for resistance to a toxin produced by P. gregata is also provided, as are cells and plants selected by this process. The invention involves culture of soybean calli in the presence of a filtrate of used fungal growth medium.
摘要翻译： 公开了一种用于筛选甘氨酸最大细胞抵抗由绿藻（Phialophora gregata），褐色茎腐病引起的疾病的抗体的体外方法。 该方法可用于鉴定棕色茎腐病大豆品种。 还提供了用于选择突变大豆细胞抵抗由灰葛草产生的毒素的方法，以及通过该方法选择的细胞和植物。 本发明涉及在所用真菌生长培养基滤液存在下培养大豆愈伤组织。
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3.发明授权For selectable markers and promoters for plant tissue culture transformation 失效用于植物组织培养转化的选择性标记和启动子
公开(公告)号：US5965727A
公开(公告)日：1999-10-12
申请号：US1826
申请日：1997-12-31
CPC分类号： C12N9/88 , C12N15/8209 , C12N15/8216 , C12N15/8222
摘要： A selectable marker, the ASA2 gene of Nicotiana tabacum, is provided for transforming plant cells. The ASA2 promoter sequence is also provided which is capable of directing tissue culture specific transcription of a downstream structural gene. Also disclosed are truncated forms of the ASA2 promoter which are capable of directing high level constitutive transcription of downstream structural genes. Constructs containing the above genes and promoters are also disclosed.
摘要翻译： 提供了一种选择性标记，即烟草烟草的ASA2基因，用于转化植物细胞。 还提供能够引导下游结构基因的组织培养特异性转录的ASA2启动子序列。 还公开了ASA2启动子的截短形式，其能够指导下游结构基因的高水平组成型转录。 还公开了含有上述基因和启动子的构建体。
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公开(公告)号：US06563025B1
公开(公告)日：2003-05-13
申请号：US09264854
申请日：1999-03-08
IPC分类号： C12N1509
CPC分类号： C12N15/8216 , C12N9/88 , C12N15/8209 , C12N15/8222
摘要： A 5-methyltryptophan resistant Nicotiana anthranilate synthase genes are disclosed, in particular the ASA2 gene of Nicotiana tabacum. Constructs containing the genes are also disclosed. This genes are useful for transforming plant cells and producing transformed plants and progenies which, compared to untransformed plant cells and plants, are more resistant to tryptophan analogs and/or produce increased levels of tryptophan. The transformed seeds are also disclosed. The genes are also useful as selectable markers. The amino acid sequence of ASA2, purified ASA2, and recombinant ASA2 are also disclosed.
摘要翻译： 公开了一种5-甲基色氨酸耐药烟碱氨基酸合酶基因，特别是烟草属的ASA2基因。 还披露了含有这些基因的构建体。 该基因可用于转化植物细胞并产生转化的植物和后代，其与未转化的植物细胞和植物相比对色氨酸类似物更有抗性和/或产生增加的色氨酸水平。 转化的种子也被披露。 这些基因也可用作选择标记。 还公开了ASA2的氨基酸序列，纯化的ASA2和重组ASA2。
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5.发明授权Whole plant regeneration via organogenesis and somaclonal variation in glycine species 失效通过器官发生和甘氨酸种类的体细胞克隆变异的整株植物再生
公开(公告)号：US4857465A
公开(公告)日：1989-08-15
申请号：US936812
申请日：1986-12-02
申请人： Usha B. Barwale , Jack M. Widholm
发明人： Usha B. Barwale , Jack M. Widholm
CPC分类号： A01H5/10 , A01H1/02 , A01H4/00 , C12N5/0025
摘要： A method is provided for the production of organogenic tissue culture and regeneration of whole plants of Glycine species, preferably Glycine max, involving the use of an organogenic culture medium comprising high cytokinin, preferably at least about 10 .mu.M BAP, and also preferably comprising at least about 6 times normal concentration of MS micronutrients. The organogenic culture medium is useful for the production of plants embodying desirable characteristics induced by somaclonal variation.
摘要翻译： 提供了一种方法，用于生产组织培养和再生整个植物的甘氨酸，优选大豆，涉及使用包含高细胞分裂素，优选至少约10μMBAP的组织培养基，并且还优选包含 至少约6倍正常浓度的MS微量营养素。 有机生长培养基可用于生产体细胞克隆变异诱导的所需特征的植物。
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