公开(公告)号：US20130023024A1

公开(公告)日：2013-01-24

申请号：US13637524

申请日：2011-03-31

申请人： Jackie Y. Ying ,  Karthikeyan Narayanan ,  Alex Wei Haw Lin ,  Andrew Chwee Aun Wan ,  Yuangang Zheng ,  Nandanan Erathodiyil

发明人： Jackie Y. Ying ,  Karthikeyan Narayanan ,  Alex Wei Haw Lin ,  Andrew Chwee Aun Wan ,  Yuangang Zheng ,  Nandanan Erathodiyil

IPC分类号： C12N13/00 ,  C12N5/071 ,  C07K7/06 ,  B82Y99/00

CPC分类号： B82Y5/00 ,  G01N33/54326 ,  G01N33/56966

摘要： A conjugate is provided for cell processing, which comprises a magnetic particle and a surface modifier having specific affinity to a target cell. The particle and modifier are linked through a cleavable peptide bond. In a method of cell processing, the conjugate is attached to a target cell; the target cell attached to the conjugate is subject to magnetic processing; the peptide bond is cleaved to separate the processed target cell from the magnetic particle; the target cell separated from the magnetic particle is attached to a substrate. The magnetic particle may include an iron oxide, and the surface modifier may include a glucosamine. The particle and modifier may be linked by a linker comprising a protease recognition site and a peptide bond. The linker links the surface modifier to the particle, and cleavage of the peptide bond is catalyzed by a specific protease that recognizes the protease recognition site.

摘要翻译： 提供了用于细胞处理的缀合物，其包含对靶细胞具有特异性亲和力的磁性颗粒和表面改性剂。 颗粒和改性剂通过可切割的肽键连接。 在细胞处理方法中，缀合物附着于靶细胞; 附着于缀合物的靶细胞进行磁加工; 肽键被切割以将加工的靶细胞与磁性颗粒分离; 从磁性颗粒分离的靶细胞附着于基材。 磁性颗粒可以包括氧化铁，并且表面改性剂可以包括葡糖胺。 颗粒和改性剂可以通过包含蛋白酶识别位点和肽键的接头连接。 接头将表面改性剂连接到颗粒上，肽键的切割由识别蛋白酶识别位点的特异性蛋白酶催化。

公开(公告)号：US20090220792A1

公开(公告)日：2009-09-03

申请号：US12087841

申请日：2006-01-20

申请人： Jackie Y. Ying ,  Yuangang Zheng

发明人： Jackie Y. Ying ,  Yuangang Zheng

IPC分类号： C30B29/60

CPC分类号： C09K11/54 ,  B82Y30/00 ,  C09K11/88 ,  C09K11/883 ,  C30B7/00 ,  C30B7/14 ,  C30B29/48 ,  Y10T428/2991

摘要： The present invention relates to nanocrystals and methods for making the same; in particular, the invention relates to ternary or higher alloyed nanocrystals and methods for making such structures in aqueous or water-soluble solvents. In certain embodiments of the invention, methods of preparing ternary or higher alloyed nanocrystals involve providing at least first, second, and third nanocrystal precursors (e.g., NaHSe, ZnCl2, and CdCl2) and forming nanocrystal structures in an aqueous or water-soluble solvent. In some cases, nanocrystal precursor solutions may also include a water-soluble ligand (e.g., glutathione, GSH). As such, ternary or higher alloyed nanocrystals (e.g., ZnxCd)—xSe) comprising the at least first, second, and third nanocrystal precursors may be formed, and the water-soluble ligand may coat at least a portion of the surface of the ternary or higher alloyed nanocrystal. Advantageously, methods for forming nanocrystals described herein can be performed at low temperatures (e.g., less than 100 degrees Celsius), and, in some embodiments, do not require the use of organic solvents. The present inventors have applied these methods to prepare blue-emitting nanocrystals with emissions that are tunable between 400-500 nm, and with quantum yields of greater than 25% in aqueous solution. These nanocrystals may be highly water soluble and can be used in a variety of applications, including those involving cell culture, sensing applications, fluorescence resonance energy transfer, and in light-emitting devices.

摘要翻译： 本发明涉及纳米晶体及其制造方法。 特别地，本发明涉及三元或更高合金化的纳米晶体和在水溶性或水溶性溶剂中制备这种结构的方法。 在本发明的某些实施方案中，制备三元或更高合金化纳米晶体的方法包括提供至少第一，第二和第三纳米晶体前体（例如NaHSe，ZnCl 2和CdCl 2）并在水溶性或水溶性溶剂中形成纳米晶体结构。 在一些情况下，纳米晶体前体溶液还可以包括水溶性配体（例如谷胱甘肽，GSH）。 因此，可以形成包含至少第一，第二和第三纳米晶体前体的三元或更高的合金化纳米晶体（例如，Zn x C d）-x Se），并且水溶性配体可以涂覆三元体表面的至少一部分 或更高的合金化纳米晶体。 有利地，本文所述的形成纳米晶体的方法可以在低温（例如，低于100摄氏度）下进行，并且在一些实施方案中不需要使用有机溶剂。 本发明人已经应用这些方法来制备具有在400-500nm之间可调谐的发射的蓝色发射纳米晶体，并且在水溶液中具有大于25％的量子产率。 这些纳米晶体可以是高度水溶性的，并且可以用于各种应用，包括涉及细胞培养，感测应用，荧光共振能量转移以及发光器件的应用。

公开(公告)号：US08097742B2

公开(公告)日：2012-01-17

申请号：US11795639

申请日：2005-01-20

申请人： Jackie Y. Ying ,  Nikhil R. Jana ,  Yuangang Zheng

发明人： Jackie Y. Ying ,  Nikhil R. Jana ,  Yuangang Zheng

IPC分类号： C07F7/08

CPC分类号： C07K17/14 ,  A61K47/6923 ,  A61K47/6929 ,  B82Y5/00 ,  C07B2200/11 ,  C07F7/1804 ,  C07F9/4012

摘要： The invention provides a process for the preparation of a surface-functionalized nanoparticle comprising: (a) reacting a nanoparticle with a functionalized silane and a base in a substantially non-aqueous solvent to obtain a partially conjugated silanated nanoparticle, wherein the functionalized silane and the base are present in relative amounts such that said functionalized silane undergoes substantially only a single hydrolysis reaction; (b) reacting the partially conjugated silanated nanoparticle formed in step (a) with a base in a solvent in which the partially conjugated silanated nanoparticle is substantially insoluble and in which the base is substantially soluble. The invention also provides a surface-functionalized nanoparticle prepared therefrom and a bioconjugate comprising said a surface-functionalized nanoparticle.

摘要翻译： 本发明提供了制备表面官能化纳米颗粒的方法，其包括：（a）使纳米颗粒与官能化的硅烷和碱在基本上非水溶剂中反应，得到部分共轭的硅烷化纳米颗粒，其中官能化的硅烷和 碱以相对量存在，使得所述官能化硅烷基本上仅经历单一水解反应; （b）使步骤（a）中形成的部分共轭的硅烷化纳米颗粒与碱在溶剂中反应，其中部分共轭的硅烷化纳米颗粒基本上不溶，其中碱基本上是可溶的。 本发明还提供由其制备的表面官能化纳米颗粒和包含所述表面官能化纳米颗粒的生物共轭物。

公开(公告)号：US20100316797A1

公开(公告)日：2010-12-16

申请号：US12866234

申请日：2009-02-04

申请人： Jackie Y. Ying ,  Yuangang Zheng ,  Yuqiong Li

发明人： Jackie Y. Ying ,  Yuangang Zheng ,  Yuqiong Li

IPC分类号： B05D7/00 ,  B05D3/02

CPC分类号： C30B29/46 ,  B82Y5/00 ,  B82Y15/00 ,  B82Y30/00 ,  C09K11/02 ,  C09K11/565 ,  C09K11/574 ,  C09K11/584 ,  C09K11/883 ,  C30B7/00 ,  C30B29/60

摘要： In a process of forming a capped crystal structure, a precursor solution is heated. The solution comprises a mixture of zinc (Zn) precursor, selenium (Se) precursor, precursor for a dopant, glutathione (GSH), and water. The dopant comprises a transition metal (M). The molar ratio of Zn:Se in the solution may be about 10:3 to about 10:5. The solution is heated for a first period sufficient to allow Zn(M)Se crystal core to form. After the first period of heating, more zinc precursor and GSH are added to the heated solution, and the solution is heated for a second period sufficient to form ZnS crystal shell on the Zn(M)Se crystal core. GSH is added in a sufficient amount to form a GSH layer around the Zn(M)Se/ZnS quantum dot.

摘要翻译： 在形成封端晶体结构的过程中，加热前体溶液。 该溶液包括锌（Zn）前体，硒（Se）前体，掺杂剂前体，谷胱甘肽（GSH）和水的混合物。 掺杂剂包含过渡金属（M）。 溶液中Zn:Se的摩尔比可以为约10:3至约10:5。 将溶液加热足以形成Zn（M）Se晶体核心的第一阶段。 在第一次加热之后，将更多的锌前体和GSH加入到加热的溶液中，并将该溶液加热足以在Zn（M）Se晶体核上形成ZnS晶体壳的第二时期。 添加足量的GSH以在Zn（M）Se / ZnS量子点周围形成GSH层。

公开(公告)号：US20080246006A1

公开(公告)日：2008-10-09

申请号：US11910305

申请日：2006-01-11

申请人： Jackie Y. Ying ,  Yuangang Zheng ,  Shujun Gao

发明人： Jackie Y. Ying ,  Yuangang Zheng ,  Shujun Gao

IPC分类号： H01L51/50 ,  C09K11/02

CPC分类号： C09K11/883 ,  B82Y10/00 ,  B82Y30/00

摘要： Quantum dots, each having a core comprising CdTe and a shell comprising GSH covering the core, are provided. The Quantum dots can be formed in a solution comprising a telluride (Te) precursor and a cadmium (Cd) precursor for forming the cores, and glutathione (GSH) for forming shells covering the cores. The cores can comprise CdTe nanocrystals grown in the solution. The growth of the nanocrystals can be limited. The quantum dots can have high fluorescence emission quantum yield such as up to about 45%, and small sizes such as from about 3.8 nm to about 6 nm.

摘要翻译： 提供了每个具有包括CdTe的核心和包含覆盖核心的GSH的外壳的量子点。 量子点可以形成在包含用于形成芯的碲化物（Te）前体和镉（Cd）前体的溶液中，并且形成覆盖芯的壳的谷胱甘肽（GSH）。 芯可以包含在溶液中生长的CdTe纳米晶体。 纳米晶体的生长可能受到限制。 量子点可以具有高的荧光发射量子产率，例如高达约45％，小尺寸例如约3.8nm至约6nm。

公开(公告)号：US20110165689A1

公开(公告)日：2011-07-07

申请号：US13057640

申请日：2009-08-04

申请人： Jackie Y. Ying ,  Jianping Xie ,  Yuangang Zheng

发明人： Jackie Y. Ying ,  Jianping Xie ,  Yuangang Zheng

IPC分类号： G01N21/64 ,  C09K11/02

CPC分类号： G01N21/643 ,  B82Y5/00 ,  B82Y15/00 ,  B82Y30/00 ,  C09K11/58 ,  G01N2021/7786

摘要： The invention relates generally to gold nanoclusters, and in particular, fluorescent gold nanoclusters. The gold nanoclusters may be stabilized, for example, with a protein or stabilizing agent. In some cases, the gold nanoclusters may be used in methods or articles to determine the presence, absence, and/or concentration of mercuric ions in a sample.

摘要翻译： 本发明一般涉及金纳米团簇，特别是荧光金纳米团簇。 金纳米团簇可以例如用蛋白质或稳定剂稳定。 在一些情况下，金纳米团簇可用于方法或制品中以确定样品中汞离子的存在，不存在和/或浓度。

公开(公告)号：US20080045736A1

公开(公告)日：2008-02-21

申请号：US11795639

申请日：2005-01-20

申请人： Jackie Ying ,  Nikhil Jana ,  Yuangang Zheng

发明人： Jackie Ying ,  Nikhil Jana ,  Yuangang Zheng

IPC分类号： C07F1/10 ,  C07F1/12 ,  C07F15/02 ,  C07F3/06 ,  C07F7/08 ,  C07F7/10 ,  C07F9/28 ,  C07K17/14

CPC分类号： C07K17/14 ,  A61K47/6923 ,  A61K47/6929 ,  B82Y5/00 ,  C07B2200/11 ,  C07F7/1804 ,  C07F9/4012

摘要： The invention provides a process for the preparation of a surface-functionalized nanoparticle comprising: (a) reacting a nanoparticle with a functionalized silane and a base in a substantially non-aqueous solvent to obtain a partially conjugated silanated nanoparticle, wherein the functionalized silane and the base are present in relative amounts such that said functionalized silane undergoes substantially only a single hydrolysis reaction; (b) reacting the partially conjugated silanated nanoparticle formed in step (a) with a base in a solvent in which the partially conjugated silanated nanoparticle is substantially insoluble and in which the base is substantially soluble. The invention also provides a surface-functionalized nanoparticle prepared therefrom and a bioconjugate comprising said a surface-functionalized nanoparticle.

摘要翻译： 本发明提供了制备表面官能化纳米颗粒的方法，其包括：（a）使纳米颗粒与官能化的硅烷和碱在基本上非水溶剂中反应，得到部分共轭的硅烷化纳米颗粒，其中官能化的硅烷和 碱以相对量存在，使得所述官能化硅烷基本上仅经历单一水解反应; （b）使步骤（a）中形成的部分共轭的硅烷化纳米颗粒与碱在溶剂中反应，其中部分共轭的硅烷化纳米颗粒基本上不溶，其中碱基本上是可溶的。 本发明还提供由其制备的表面官能化纳米颗粒和包含所述表面官能化纳米颗粒的生物共轭物。