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公开(公告)号：US20100041100A1
公开(公告)日：2010-02-18
申请号：US12512403
申请日：2009-07-30
CPC分类号： C12N9/2437
摘要： A modified Trichoderma reesei Family 6 (TrCel6A) cellulase enzyme comprising amino acid substitutions at one or more positions selected from the group consisting of 129, 322, 363 and 410 of SEQ ID NO: 1 is provided. Genetic constructs and genetically modified microbes comprising nucleic sequences encoding the modified TrCel6a cellulase are also provided. The modified TrCel6A cellulase of the invention display at least a 15% decrease in inactivation by lignin relative to a parental TrCel6A cellulase from which the modified TrCel6A is derived. Such cellulases find use in a variety of applications in industry requiring enzymatic hydrolysis of cellulose in the presence of lignin, e.g., the hydrolysis of pretreated lignocellulosic feedstocks for the production of fermentable sugars, sugar alcohols and fuel alcohols.
摘要翻译： 提供了修饰的里氏木霉家族6（TrCel6A）纤维素酶，其在选自SEQ ID NO：1的129,322,363和410的一个或多个位置上包含氨基酸取代。 还提供了包含编码修饰的TrCel6a纤维素酶的核酸序列的遗传构建体和遗传修饰微生物。 本发明的经修饰的TrCel6A纤维素酶相对于来自其的TrCel6A衍生的亲本TrCel6A纤维素酶显示出至少15％的木质素灭活降低。 这种纤维素酶可用于需要在木质素存在下酶促水解纤维素的工业中的各种应用，例如用于生产可发酵糖，糖醇和燃料醇的预处理的木质纤维素原料的水解。
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公开(公告)号：US08110389B2
公开(公告)日：2012-02-07
申请号：US12512403
申请日：2009-07-30
IPC分类号： C12N9/42 , C12N9/14 , C12N15/00 , C12N1/20 , C12N1/00 , C12P9/04 , C12P21/06 , C12P19/34 , C02F3/34 , D21C1/00 , C07H21/04
CPC分类号： C12N9/2437
摘要： A modified Trichoderma reesei Family 6 (TrCel6A) cellulase enzyme comprising amino acid substitutions at one or more positions selected from the group consisting of 129, 322, 363 and 410 of SEQ ID NO: 1 is provided. Genetic constructs and genetically modified microbes comprising nucleic sequences encoding the modified TrCel6a cellulase are also provided. The modified TrCel6A cellulase of the invention display at least a 15% decrease in inactivation by lignin relative to a parental TrCel6A cellulase from which the modified TrCel6A is derived. Such cellulases find use in a variety of applications in industry requiring enzymatic hydrolysis of cellulose in the presence of lignin, e.g., the hydrolysis of pretreated lignocellulosic feedstocks for the production of fermentable sugars, sugar alcohols and fuel alcohols.
摘要翻译： 提供了修饰的里氏木霉家族6（TrCel6A）纤维素酶，其在选自SEQ ID NO：1的129,322,363和410的一个或多个位置上包含氨基酸取代。 还提供了包含编码修饰的TrCel6a纤维素酶的核酸序列的遗传构建体和遗传修饰微生物。 本发明的经修饰的TrCel6A纤维素酶相对于来自其的TrCel6A衍生的亲本TrCel6A纤维素酶显示出至少15％的木质素灭活降低。 这种纤维素酶可用于需要在木质素存在下酶促水解纤维素的工业中的各种应用，例如用于生产可发酵糖，糖醇和燃料醇的预处理的木质纤维素原料的水解。
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公开(公告)号：US08143049B2
公开(公告)日：2012-03-27
申请号：US12550615
申请日：2009-08-31
CPC分类号： C12P19/14 , C12N9/2445 , C12Y302/01021
摘要： Provided are modified beta-glucosidase enzymes, derived from the Trichoderma reesei Cel3A beta-glucosidase, that exhibit improved stability at low pH, low pH and high aeration, low pH and high agitation, or low pH and elevated temperature. Also provided are genetic constructs comprising nucleotide sequences encoding for modified beta-glucosidase enzymes, methods for the production of modified beta-glucosidase enzymes from host strains and the use of the modified beta-glucosidase enzymes in the hydrolysis of cellulose.
摘要翻译： 提供了源自里氏木霉Cel3Aβ-葡糖苷酶的修饰的β-葡糖苷酶，其在低pH，低pH和高通气，低pH和高搅拌，或低pH和升高的温度下表现出改善的稳定性。 还提供了包含编码修饰的β-葡糖苷酶的核苷酸序列的遗传构建体，用于从宿主菌株产生修饰的β-葡糖苷酶的方法和在纤维素水解中使用经修饰的β-葡糖苷酶。
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公开(公告)号：US20100093040A1
公开(公告)日：2010-04-15
申请号：US12550615
申请日：2009-08-31
CPC分类号： C12P19/14 , C12N9/2445 , C12Y302/01021
摘要： Provided are modified beta-glucosidase enzymes, derived from the Trichoderma reesei Cel3A beta-glucosidase, that exhibit improved stability at low pH, low pH and high aeration, low pH and high agitation, or low pH and elevated temperature. Also provided are genetic constructs comprising nucleotide sequences encoding for modified beta-glucosidase enzymes, methods for the production of modified beta-glucosidase enzymes from host strains and the use of the modified beta-glucosidase enzymes in the hydrolysis of cellulose.
摘要翻译： 提供了源自里氏木霉Cel3Aβ-葡糖苷酶的修饰的β-葡糖苷酶，其在低pH，低pH和高通气，低pH和高搅拌，或低pH和升高的温度下表现出改善的稳定性。 还提供了包含编码修饰的β-葡糖苷酶的核苷酸序列的遗传构建体，用于从宿主菌株产生修饰的β-葡糖苷酶的方法和在纤维素水解中使用经修饰的β-葡糖苷酶。
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