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    Methods of selecting internalizing antibodies
    2.
    发明授权
    Methods of selecting internalizing antibodies 失效
    选择内化抗体的方法

    公开(公告)号：US06794128B2

    公开(公告)日：2004-09-21

    申请号：US09249529

    申请日：1999-02-12

    Applicant: James D. Marks ,  Marie Alix Poul ,  Baltazar Becerril

    Inventor： James D. Marks ,  Marie Alix Poul ,  Baltazar Becerril

    IPC: C12Q100

    CPC classification number: C12N15/1037 ,  A61K38/00 ,  A61K47/6843 ,  C07K14/005 ,  C07K16/2863 ,  C07K16/2881 ,  C07K16/32 ,  C07K2317/21 ,  C07K2317/622 ,  C07K2317/626 ,  C07K2317/77 ,  C07K2319/02 ,  C12N15/86 ,  C12N2795/10022

    Abstract: This invention provides methods of selecting antibodies that are internalized into target cells. The methods generally involve contacting target cells with one or more members of an antibody phage display library. The members of the phage display library are also contacted with cells of a subtractive cell line. The target cells are then washed to remove the subtractive cell line cells and members of the phage display library that are non-specifically bound or weakly bound to the target cells. The target cells are cultured under conditions where members of the phage display library can be internalized if bound to an internalizing marker and internalized members of the phage display library are then identified.

    Abstract translation: 本发明提供了选择内化于靶细胞中的抗体的方法。 所述方法通常涉及使靶细胞与抗体噬菌体展示文库的一个或多个成员接触。 噬菌体展示文库的成员也与减数细胞系的细胞接触。 然后洗涤靶细胞以除去非特异性结合或弱结合到靶细胞的消减细胞系细胞和噬菌体展示文库的成员。 靶细胞在噬菌体展示文库的成员可以内化的条件下培养，如果结合到内化标记物，然后鉴定噬菌体展示文库的内化成员。

    ANTIBODIES DIRECTED AGAINST THE TRANSFERRIN RECEPTOR AND USES THEREOF FOR IMMUNOTHERAPY OF IRON-DEPENDENT TUMOURS
    5.
    发明申请
    ANTIBODIES DIRECTED AGAINST THE TRANSFERRIN RECEPTOR AND USES THEREOF FOR IMMUNOTHERAPY OF IRON-DEPENDENT TUMOURS 审中-公开
    针对转铁蛋白受体的抗体及其依赖于依赖于肿瘤的免疫球蛋白的用途

    公开(公告)号：US20130045206A1

    公开(公告)日：2013-02-21

    申请号：US13515551

    申请日：2010-12-16

    Applicant: Marie-Alix Poul ,  Ronan Philippe Crepin ,  James D. Marks

    Inventor： Marie-Alix Poul ,  Ronan Philippe Crepin ,  James D. Marks

    IPC: C07K16/28 ,  C12N15/63 ,  A61P37/06 ,  C12N5/10 ,  A61K39/395 ,  A61P35/00 ,  C12N15/13 ,  C12N1/21

    CPC classification number: C07K16/2881 ,  A61K2039/505 ,  C07K16/30 ,  C07K2317/21 ,  C07K2317/622 ,  C07K2317/73 ,  C07K2317/76

    Abstract: The present invention relates to a molecular structure characterised in that said structure includes at least one amino acid sequence selected from: SEQ. ID NO.: 1, SEQ. ID NO.: 2, SEQ. ID NO.: 3, SEQ. ID NO.: 4, SEQ. ID NO.: 5, SEQ. ID NO.: 6, SEQ. ID NO.: 7, SEQ. ID NO.: 8, SEQ. ID NO.: 9, SEQ. ID NO.: 10, SEQ. ID NO.: 11, SEQ. ID NO.: 12, SEQ. ID NO.: 13, SEQ. ID NO.: 14, SEQ. ID NO.: 15, SEQ. ID NO.: 16, SEQ. ID NO.: 17, SEQ. ID NO.: 18, SEQ. ID NO.: 19, SEQ. ID NO.: 20, SEQ. ID NO.: 21, SEQ. ID NO.: 22, SEQ. ID NO.: 23, SEQ. ID NO.: 24, SEQ. ID NO.: 25, SEQ. ID NO.: 26, SEQ. ID NO.: 27, SEQ. ID NO.: 28, SEQ. ID NO.: 29, SEQ. ID NO.: 30, SEQ. ID NO.: 31, SEQ. ID NO.: 32, SEQ. ID NO.: 33, SEQ. ID NO.: 34, SEQ. ID NO.: 35, SEQ. ID NO.: 36, said amino acid sequence corresponding to an antigen complementarity determining region (CDR) of the variable domain of the heavy chain (CDR-H) or the light chain (CDR-L) of an antibody targeting the human transferrin receptor (TfR). The present invention also relates to a pharmaceutical composition including a therapeutically effective amount of at least one molecular structure as defined in the present application, combined with a pharmaceutically acceptable carrier.

    Abstract translation: 本发明涉及分子结构，其特征在于所述结构包括至少一个选自以下的氨基酸序列：SEQ。 编号：1，SEQ。 编号：2，SEQ。 编号：3，SEQ。 编号：4，SEQ。 编号：5，SEQ。 编号：6，SEQ。 SEQ ID NO：7，SEQ。 SEQ ID NO：8，SEQ。 编号：9，SEQ。 编号：10，SEQ。 编号：11，SEQ。 SEQ ID NO：12，SEQ。 SEQ ID NO：13，SEQ。 SEQ ID NO：14，SEQ。 编号：15，SEQ。 编号：16，SEQ。 编号：17，SEQ。 编号：18，SEQ。 编号：19，SEQ。 SEQ ID NO：20，SEQ。 编号：21，SEQ。 编号：22，SEQ。 编号：23，SEQ。 SEQ ID NO：24，SEQ。 编号：25，SEQ。 编号：26，SEQ。 编号：27，SEQ。 编号：28，SEQ。 编号：29，SEQ。 SEQ ID NO：30，SEQ。 编号：31，SEQ。 编号：32，SEQ。 编号：33，SEQ。 编号：34，SEQ。 编号：35，SEQ。 SEQ ID NO：36，所述氨基酸序列对应于针对人转铁蛋白受体的抗体的重链（CDR-H）或轻链（CDR-L）的可变结构域的抗原互补决定区（CDR） （TfR）。 本发明还涉及药物组合物，其包含治疗有效量的至少一种如本申请中所定义的分子结构与药学上可接受的载体组合。

    Methods of selecting internalizing antibodies
    10.
    发明申请
    Methods of selecting internalizing antibodies 审中-公开
    选择内化抗体的方法

    公开(公告)号：US20090105086A1

    公开(公告)日：2009-04-23

    申请号：US12154688

    申请日：2008-05-23

    Applicant: James D. Marks ,  Marie Alix Poul ,  Baltazar Becerril

    Inventor： James D. Marks ,  Marie Alix Poul ,  Baltazar Becerril

    IPC: C40B30/04

    CPC classification number: C12N15/1037 ,  A61K38/00 ,  A61K47/6843 ,  C07K14/005 ,  C07K16/2863 ,  C07K16/2881 ,  C07K16/32 ,  C07K2317/21 ,  C07K2317/622 ,  C07K2317/626 ,  C07K2317/77 ,  C07K2319/02 ,  C12N15/86 ,  C12N2795/10022

    Abstract: This invention provides methods of selecting antibodies that are internalized into target cells. The methods generally involve contacting target cells with one or more members of an antibody phage display library. The members of the phage display library are also contacted with cells of a subtractive cell line. The target cells are then washed to remove the subtractive cell line cells and members of the phage display library that are non-specifically bound or weakly bound to the target cells. The target cells are cultured under conditions where members of the phage display library can be internalized if bound to an internalizing marker and internalized members of the phage display library are then identified.

    Abstract translation: 本发明提供了选择内化于靶细胞中的抗体的方法。 所述方法通常涉及使靶细胞与抗体噬菌体展示文库的一个或多个成员接触。 噬菌体展示文库的成员也与减数细胞系的细胞接触。 然后洗涤靶细胞以除去非特异性结合或弱结合到靶细胞的消减细胞系细胞和噬菌体展示文库的成员。 靶细胞在噬菌体展示文库的成员可以内化的条件下培养，如果结合到内化标记物，然后鉴定噬菌体展示文库的内化成员。

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