公开(公告)号：US09145462B2

公开(公告)日：2015-09-29

申请号：US13172759

申请日：2011-06-29

Applicant: Bin Liu ,  James D. Marks ,  Xiaodong Zhu ,  Scott Bidlingmaier

Inventor： Bin Liu ,  James D. Marks ,  Xiaodong Zhu ,  Scott Bidlingmaier

IPC: A61K39/395 ,  C07K16/28 ,  C07K16/30 ,  C12N5/095 ,  G01N33/574

CPC classification number: C07K16/18 ,  A61K47/6843 ,  A61K51/1045 ,  C07K16/2896 ,  C07K16/3053 ,  C07K2317/21 ,  C07K2317/54 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2317/73 ,  C07K2317/77 ,  C07K2317/92 ,  C12N5/0695 ,  G01N33/57407

Abstract: The present disclosure is generally related to antibodies that bind specifically to glioblastoma multiforme (GBM) cells. In particular, the present disclosure provides compositions comprising human single chain or full-length antibodies that bind tumor cells. Additionally the present disclosure provides methods of using the anti-GBM antibodies.

Abstract translation: 本公开通常涉及特异性结合多形性胶质母细胞瘤（GBM）细胞的抗体。 特别地，本公开提供了包含结合肿瘤细胞的人单链或全长抗体的组合物。 另外，本公开提供了使用抗GBM抗体的方法。

公开(公告)号：US20150030600A1

公开(公告)日：2015-01-29

申请号：US13813623

申请日：2011-08-31

Applicant: James D. Marks ,  Yongfeng Fan ,  Jianlong Lou ,  Maria Consuelo Garcia Rodriguez ,  Shude Yan ,  Isin Nergiz Geren ,  Wenwu Zhai ,  Subhendu Chakraborti

Inventor： James D. Marks ,  Yongfeng Fan ,  Jianlong Lou ,  Maria Consuelo Garcia Rodriguez ,  Shude Yan ,  Isin Nergiz Geren ,  Wenwu Zhai ,  Subhendu Chakraborti

IPC: C07K16/12 ,  G01N33/569 ,  G01N33/573

CPC classification number: C07K16/40 ,  A61K2039/505 ,  A61K2039/507 ,  C07K16/1282 ,  C07K2317/20 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/33 ,  C07K2317/54 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/76 ,  C07K2317/92 ,  G01N33/56911 ,  G01N33/573 ,  G01N2333/33 ,  G01N2333/952

Abstract: The present disclosure provides antibodies that specifically bind to botulinum neurotoxins (e.g., BoNT/A, BoNT/B, BoNT/C, BoNT/D, BoNT/E, BoNT/F, BoNT/G, etc.) and the epitopes bound by those antibodies. The antibodies and derivatives thereof that specifically bind to the neutralizing epitopes provided herein can be used to neutralize botulinum neurotoxin and are therefore also useful in the treatment of botulism.

Abstract translation: 本公开提供了特异性结合肉毒杆菌神经毒素（例如BoNT / A，BoNT / B，BoNT / C，BoNT / D，BoNT / E，BoNT / F，BoNT / G等）的抗体， 那些抗体。 特异性结合本文提供的中和表位的抗体及其衍生物可用于中和肉毒杆菌神经毒素，因此也可用于治疗肉毒中毒。

公开(公告)号：US08263747B2

公开(公告)日：2012-09-11

申请号：US12353237

申请日：2009-01-13

Applicant: James D. Marks ,  Peter Amersdorfer ,  Ali Razai

Inventor： James D. Marks ,  Peter Amersdorfer ,  Ali Razai

IPC: A61K38/00 ,  A61K38/16 ,  A61K39/395 ,  A61K39/40 ,  A61K39/42 ,  A01N37/18 ,  C07K16/00 ,  C12P21/08 ,  G01N33/00

CPC classification number: C07K16/1282 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/54 ,  C07K2317/55 ,  C07K2317/622

Abstract: This invention provides antibodies that specifically bind to and neutralize botulinum neurotoxin type A (BoNT/A) and the epitopes bound by those antibodies. The antibodies and derivatives thereof and/or other antibodies that specifically bind to the neutralizing epitopes provided herein can be used to neutralize botulinum neurotoxin and are therefore also useful in the treatment of botulism.

公开(公告)号：US07892554B2

公开(公告)日：2011-02-22

申请号：US11809773

申请日：2007-06-01

Applicant: James D. Marks ,  Marie Alix Poul

Inventor： James D. Marks ,  Marie Alix Poul

IPC: A61K39/395 ,  A61K51/10 ,  C07K16/46

CPC classification number: A61K47/48823 ,  A61K9/0019 ,  A61K31/704 ,  A61K38/00 ,  A61K47/6809 ,  A61K47/6843 ,  A61K47/6851 ,  A61K47/6855 ,  A61K47/6865 ,  A61K47/6913 ,  C07K16/2863 ,  C07K16/2881 ,  C07K16/3053 ,  C07K16/32 ,  C07K2317/10 ,  C07K2317/21 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/626 ,  C07K2317/77 ,  C07K2317/92 ,  C07K2319/02 ,  C12N15/86 ,  C12N2795/10043 ,  C12N2810/859 ,  Y10S424/812

Abstract: This invention provides novel erbB2-binding internalizing antibodies. The antibodies, designated F5 and C1, specifically bind to c-erbB2 antigen and, upon binding, are readily internalized into the cell bearing the c-erbB2 marker. Chimeric molecules comprising the F5 and/or C1 antibodies attached to one or more effector molecules are also provided.

公开(公告)号：US07582736B2

公开(公告)日：2009-09-01

申请号：US11972130

申请日：2008-01-10

Applicant: Bin Liu ,  James D. Marks

Inventor： Bin Liu ,  James D. Marks

IPC: C12P21/08

CPC classification number: C07K16/3069 ,  A61K2039/505 ,  C07K2317/622 ,  C07K2317/73 ,  C07K2317/77

Abstract: This invention provides novel prostate cancer specific internalizing human antibodies. The antibodies are useful by themselves to prevent growth and/or proliferation of prostate cancer cells. The antibodies can also be formulated as chimeric molecules to direct an effector (e.g. a cytotoxin, an imaging reagent, a drug, etc.) to a prostate tumor site.

Abstract translation: 本发明提供了新型前列腺癌特异性内化人抗体。 抗体本身可用于预防前列腺癌细胞的生长和/或增殖。 抗体还可以配制成嵌合分子以将效应物（例如细胞毒素，成像试剂，药物等）引导至前列腺肿瘤部位。

公开(公告)号：US20080124328A1

公开(公告)日：2008-05-29

申请号：US11342271

申请日：2006-01-26

Applicant: James D. Marks ,  Peter Amersdorfer ,  Isin Geren ,  Jianlong Lou ,  Ali Razai ,  Maria Consuelos Garcia

Inventor： James D. Marks ,  Peter Amersdorfer ,  Isin Geren ,  Jianlong Lou ,  Ali Razai ,  Maria Consuelos Garcia

IPC: A61K39/40 ,  A61K39/395 ,  A61P43/00 ,  C07H19/00 ,  C07K16/00 ,  C12N1/00 ,  C12N5/00

CPC classification number: C07K16/1282 ,  A61K2039/507 ,  C07K2317/21 ,  C07K2317/622 ,  C07K2317/76 ,  C07K2317/92

Abstract: This invention provides antibodies that specifically bind to and neutralize botulinum neurotoxin type A (BoNT/A) and the epitopes bound by those antibodies. The antibodies and derivatives thereof and/or other antibodies that specifically bind to the neutralizing epitopes provided herein can be used to neutralize botulinum neurotoxin and are therefore also useful in the treatment of botulism.

Abstract translation: 本发明提供了特异性结合并中和A型（BoNT / A）肉毒杆菌神经毒素和由这些抗体结合的表位的抗体。 特异性结合本文提供的中和表位的抗体及其衍生物和/或其他抗体可用于中和肉毒杆菌神经毒素，因此也可用于治疗肉毒中毒。

公开(公告)号：US07335744B2

公开(公告)日：2008-02-26

申请号：US11021438

申请日：2004-12-21

Applicant: Bin Liu ,  James D. Marks

Inventor： Bin Liu ,  James D. Marks

IPC: C12P21/08

CPC classification number: C07K16/3069 ,  A61K2039/505 ,  C07K2317/622 ,  C07K2317/73 ,  C07K2317/77

Abstract: This invention provides novel prostate cancer specific internalizing human antibodies. The antibodies are useful by themselves to prevent growth and/or proliferation of prostate cancer cells. The antibodies can also be formulated as chimeric molecules to direct an effector (e.g. a cytotoxin, an imaging reagent, a drug, etc.) to a prostate tumor site.

Abstract translation: 本发明提供了新型前列腺癌特异性内化人抗体。 抗体本身可用于预防前列腺癌细胞的生长和/或增殖。 抗体还可以配制成嵌合分子以将效应物（例如细胞毒素，成像试剂，药物等）引导至前列腺肿瘤部位。

公开(公告)号：US06794128B2

公开(公告)日：2004-09-21

申请号：US09249529

申请日：1999-02-12

Applicant: James D. Marks ,  Marie Alix Poul ,  Baltazar Becerril

Inventor： James D. Marks ,  Marie Alix Poul ,  Baltazar Becerril

IPC: C12Q100

CPC classification number: C12N15/1037 ,  A61K38/00 ,  A61K47/6843 ,  C07K14/005 ,  C07K16/2863 ,  C07K16/2881 ,  C07K16/32 ,  C07K2317/21 ,  C07K2317/622 ,  C07K2317/626 ,  C07K2317/77 ,  C07K2319/02 ,  C12N15/86 ,  C12N2795/10022

Abstract: This invention provides methods of selecting antibodies that are internalized into target cells. The methods generally involve contacting target cells with one or more members of an antibody phage display library. The members of the phage display library are also contacted with cells of a subtractive cell line. The target cells are then washed to remove the subtractive cell line cells and members of the phage display library that are non-specifically bound or weakly bound to the target cells. The target cells are cultured under conditions where members of the phage display library can be internalized if bound to an internalizing marker and internalized members of the phage display library are then identified.

Abstract translation: 本发明提供了选择内化于靶细胞中的抗体的方法。 所述方法通常涉及使靶细胞与抗体噬菌体展示文库的一个或多个成员接触。 噬菌体展示文库的成员也与减数细胞系的细胞接触。 然后洗涤靶细胞以除去非特异性结合或弱结合到靶细胞的消减细胞系细胞和噬菌体展示文库的成员。 靶细胞在噬菌体展示文库的成员可以内化的条件下培养，如果结合到内化标记物，然后鉴定噬菌体展示文库的内化成员。

公开(公告)号：US09243057B2

公开(公告)日：2016-01-26

申请号：US13813623

申请日：2011-08-31

Applicant: James D. Marks ,  Maria Consuelo Garcia Rodriguez

Inventor： James D. Marks ,  Maria Consuelo Garcia Rodriguez

IPC: C07K16/12 ,  G01N33/569 ,  G01N33/573 ,  A61K39/00

CPC classification number: C07K16/40 ,  A61K2039/505 ,  A61K2039/507 ,  C07K16/1282 ,  C07K2317/20 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/33 ,  C07K2317/54 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/76 ,  C07K2317/92 ,  G01N33/56911 ,  G01N33/573 ,  G01N2333/33 ,  G01N2333/952

Abstract: The present disclosure provides antibodies that specifically bind to botulinum neurotoxins (e.g., BoNT/A, BoNT/B, BoNT/C, BoNT/D, BoNT/E, BoNT/F, BoNT/G, etc.) and the epitopes bound by those antibodies. The antibodies and derivatives thereof that specifically bind to the neutralizing epitopes provided herein can be used to neutralize botulinum neurotoxin and are therefore also useful in the treatment of botulism.

Abstract translation: 本公开提供了特异性结合肉毒杆菌神经毒素（例如BoNT / A，BoNT / B，BoNT / C，BoNT / D，BoNT / E，BoNT / F，BoNT / G等）的抗体， 那些抗体。 特异性结合本文提供的中和表位的抗体及其衍生物可用于中和肉毒杆菌神经毒素，因此也可用于治疗肉毒中毒。

公开(公告)号：US08865873B2

公开(公告)日：2014-10-21

申请号：US12724282

申请日：2010-03-15

Applicant: Bin Liu ,  James D. Marks

Inventor： Bin Liu ,  James D. Marks

IPC: C07K16/30 ,  A61K51/10 ,  C07K16/28

CPC classification number: C07K16/3069 ,  A61K51/1072 ,  A61K2039/505 ,  C07K16/005 ,  C07K16/18 ,  C07K16/2803 ,  C07K16/30 ,  C07K2317/21 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/569 ,  C07K2317/622 ,  C07K2317/77 ,  G01N33/5044 ,  G01N33/57434 ,  G01N33/6845 ,  G01N33/6854

Abstract: This invention provides a method that allows selection of antibodies against cells (e.g., tumor cells) in situ using laser capture microdissection. By restricting antibody selection to binders of internalizing epitopes, a panel of phage antibodies was generated that targets clinically represented prostate cancer antigens.

Abstract translation: 本发明提供允许使用激光捕获显微切割原位选择针对细胞（例如肿瘤细胞）的抗体的方法。 通过将抗体选择限于内化表位的结合物，产生一组靶向临床表现的前列腺癌抗原的噬菌体抗体。