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1.发明授权Nucleic acid amplification oligonucleotides and probes to Lyme disease associated Borrelia 失效核酸扩增寡核苷酸和探针与莱姆病相关的疏螺旋体
公开(公告)号：US6074826A
公开(公告)日：2000-06-13
申请号：US953094
申请日：1997-10-17
申请人： James J. Hogan , Yeasing Yang , Nick Carter
发明人： James J. Hogan , Yeasing Yang , Nick Carter
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , C12R1/01 , G01N33/569 , G01N37/00 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/689
摘要： The present invention discloses hybridization assay probes, amplification primers, nucleic acid compositions and methods useful for detecting Borrelia nucleic acids. Hybridization assay probes and amplification primers that selectively detect Lyme disease-associated Borrelia and distinguish those Borrelia from Borrelia hermsii are disclosed. Other hybridization probes selectively detect Borrelia hermsii and not Lyme disease-associated Borrelia are also described.
摘要翻译： 本发明公开了杂交测定探针，扩增引物，核酸组合物和用于检测波氏杆菌核酸的方法。 公开了选择性检测莱姆病相关的疏螺旋体并区分这些疏螺旋体的杂交测定探针和扩增引物。 还描述了其他杂交探针选择性检测绵羊杆菌，而不是莱姆病相关的疏螺旋体。
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公开(公告)号：US20090305293A1
公开(公告)日：2009-12-10
申请号：US12539518
申请日：2009-08-11
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6895
摘要： Compositions are disclosed as nucleic acid sequences that may be used as amplification oligomers, including primers, capture probes for sample preparation, and detection probes specific for Candida albicans 26S rRNA sequences or DNA encoding 26S rRNA. Methods are disclosed for detecting the presence of C. albicans in samples by using the disclosed compositions in methods that include in vitro nucleic acid amplification of a 26S rRNA sequence or DNA encoding the 26S rRNA sequence to produce a detectable amplification product.
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3.发明授权Polynucleotide probes for detection and quantitation of bacteria in the family enterobacteriaceae 有权用于肠杆菌科家族中细菌检测和定量的多核苷酸探针公开(公告)号：US06326486B1
公开(公告)日：2001-12-04
申请号：US09565156
申请日：2000-05-03
申请人： James J. Hogan , Patricia Gordon
发明人： James J. Hogan , Patricia Gordon
IPC分类号： C07H2104
CPC分类号： C12Q1/689 , Y02A50/451 , Y02A50/59
摘要： Polynucleotides useful for specifically detecting bacteria in the family Enterobacteriaceae. Probes and accessory “helper oligonucleotides” are disclosed for use in hybridizing the rRNA and rDNA of Enterobacteriaceae without substantially cross-hybridizing with the rRNA or rDNA of numerous other bacterial and fungal species.
摘要翻译： 用于特异性检测肠杆菌科菌的多核苷酸。 公开了探针和辅助“辅助寡核苷酸”用于杂交肠杆菌科的rRNA和rDNA，而与许多其它细菌和真菌物种的rRNA或rDNA基本上不杂交。
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公开(公告)号：US5030557A
公开(公告)日：1991-07-09
申请号：US124975
申请日：1987-11-24
申请人： James J. Hogan , Curt L. Milliman
发明人： James J. Hogan , Curt L. Milliman
CPC分类号： C12Q1/12 , C12Q1/6832 , C12Q1/689 , Y10S435/81
摘要： The binding of a nucleic acid probe with its complementary sequence in a targeted, single stranded nucleic acid is affected by the secondary and tertiary structure of the target nucleic acid. The rate and extent of hybridization of the probe with the targeted nucleic acid can be increased by the use of "helper" oligonucleotides. Helper oligonucleotides are selected to bind to the target nucleic acid and impose a different secondary and tertiary structure on the target to facilitate the binding of the probe to the target. The resulting hybrid of probe and target nucleic acid also exhibits a higher T.sub.m than the hybrid which results from addition of the probe alone.
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5.发明授权Compositions, kits and related methods for the detection and/or monitoring of Listeria 有权用于检测和/或监测李斯特菌的组合物，试剂盒和相关方法公开(公告)号：US08748133B2
公开(公告)日：2014-06-10
申请号：US12649249
申请日：2009-12-29
IPC分类号： C12P19/34
CPC分类号： C12Q1/689 , C12Q1/6816 , C12Q1/6865
摘要： Provided are compositions, kits, and methods for the identification of Listeria. In certain aspects and embodiments, the compositions, kits, and methods may provide improvements in relation to specificity, sensitivity, and speed of detection.
摘要翻译： 提供用于鉴定李斯特菌的组合物，试剂盒和方法。 在某些方面和实施方案中，组合物，试剂盒和方法可以提供与特异性，灵敏度和检测速度有关的改进。
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6.发明授权Compositions, kits and related methods for the detection and/or monitoring of Salmonella 有权用于检测和/或监测沙门氏菌的组合物，试剂盒和相关方法公开(公告)号：US08703421B2
公开(公告)日：2014-04-22
申请号：US12995168
申请日：2009-05-29
CPC分类号： C12Q1/68 , C12Q1/689 , C12Q2304/00 , Y02A50/451
摘要： Provided are compositions, kits, and methods for the identification of Salmonella. In certain aspects and embodiments, the compositions, kits, and methods may provide improvements in relation to specificity, sensitivity, and speed of detection.
摘要翻译： 提供用于鉴定沙门氏菌的组合物，试剂盒和方法。 在某些方面和实施方案中，组合物，试剂盒和方法可以提供与特异性，灵敏度和检测速度有关的改进。
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7.发明授权公开(公告)号：US08293885B2
公开(公告)日：2012-10-23
申请号：US11931469
申请日：2007-10-31
CPC分类号： C12Q1/689
摘要： The invention includes compositions and methods of detection of Bacillus anthracis that use oligonucleotide probes specific for genetic material contained in the pXO1 and pXO2 plasmids in nucleic acid hybridization reactions. Embodiments of the method may include additional probes specific for other gene sequences to distinguish B. anthracis from other bacterial species present in a sample or to provide an indication that the assay was performed properly even when no Bacillus sequence is detected.
摘要翻译： 本发明包括在核酸杂交反应中使用对包含在pXO1和pXO2质粒中的遗传物质特异的寡核苷酸探针的炭疽芽孢杆菌的检测方法。 该方法的实施方案可以包括对于其他基因序列特异性的另外的探针，以区分来自样品中存在的其它细菌种类的炭疽芽孢杆菌，或提供即使没有检测到芽孢杆菌序列也能够适当地进行测定的指示。
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8.发明申请公开(公告)号：US20120171669A1
公开(公告)日：2012-07-05
申请号：US11931469
申请日：2007-10-31
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689
摘要： The invention includes compositions and methods of detection of Bacillus anthracis that use oligonucleotide probes specific for genetic material contained in the pXO1 and pXO2 plasmids in nucleic acid hybridization reactions. Embodiments of the method may include additional probes specific for other gene sequences to distinguish B. anthracis from other bacterial species present in a sample or to provide an indication that the assay was performed properly even when no Bacillus sequence is detected.
摘要翻译： 本发明包括在核酸杂交反应中使用对包含在pXO1和pXO2质粒中的遗传物质特异的寡核苷酸探针的炭疽芽孢杆菌的检测方法。 该方法的实施方案可以包括对于其他基因序列特异性的另外的探针，以区分来自样品中存在的其它细菌种类的炭疽芽孢杆菌，或提供即使没有检测到芽孢杆菌序列也能够适当地进行测定的指示。
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9.发明申请COMPOSITIONS AND METHODS TO DETECT LEGIONELLA PNEUMOPHILA NUCLEIC ACID 审中-公开组合物和检测LEGIONELLA PNEUMOPHILA核酸的方法公开(公告)号：US20090291431A1
公开(公告)日：2009-11-26
申请号：US11582770
申请日：2006-10-17
申请人： Jennifer J. Bungo , James J. Hogan , Reinhold B. Pollner , Marie K. Hudspeth , Shannon K. Kaplan , Elizabeth M. Marlowe
发明人： Jennifer J. Bungo , James J. Hogan , Reinhold B. Pollner , Marie K. Hudspeth , Shannon K. Kaplan , Elizabeth M. Marlowe
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , Y02A50/451
摘要： Compositions are disclosed as nucleic acid sequences that may be used as amplification oligomers, including primers, capture probes for sample preparation, and detection probes specific for Legionella pneumophila 16S or 23S rRNA sequences or DNA encoding 16S or 23S rRNA. Methods are disclosed for detecting the presence of L. pnuemophila in samples by using the disclosed compositions in methods that include in vitro nucleic acid amplification of a 16S rRNA sequence or DNA encoding the 16S rRNA sequence, or of a 23S rRNA sequence or DNA encoding the 23S rRNA sequence to produce a detectable amplification product.
摘要翻译： 公开了组合物作为可用作扩增寡聚体的核酸序列，包括引物，用于样品制备的捕获探针，以及针对嗜肺军团菌16S或23S rRNA序列或编码16S或23S rRNA的DNA特异的检测探针。 公开了通过使用所公开的组合物在包括体外核酸扩增16S rRNA序列或编码16S rRNA序列的DNA或编码23S rRNA序列或编码16S rRNA序列的DNA的方法中来检测样品中嗜血杆菌的存在的方法 23S rRNA序列以产生可检测的扩增产物。
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公开(公告)号：US07595164B2
公开(公告)日：2009-09-29
申请号：US12335356
申请日：2008-12-15
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6895
摘要： Compositions are disclosed as nucleic acid sequences that may be used as amplification oligomers, including primers, capture probes for sample preparation, and detection probes specific for Candida albicans 26S rRNA sequences or DNA encoding 26S rRNA. Methods are disclosed for detecting the presence of C. albicans in samples by using the disclosed compositions in methods that include in vitro nucleic acid amplification of a 26S rRNA sequence or DNA encoding the 26S rRNA sequence to produce a detectable amplification product.
摘要翻译： 公开了组合物作为可用作扩增寡聚体的核酸序列，包括引物，用于样品制备的捕获探针和对白色念珠菌26S rRNA序列或编码26S rRNA的DNA特异的检测探针。 公开了通过使用所公开的组合物在包括26S rRNA序列的体外核酸扩增或编码26S rRNA序列的DNA以产生可检测的扩增产物的方法中来检测样品中白色念珠菌存在的方法。
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