利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

41 条记录 (耗时 0.043 秒)

    Isolating cells expressing secreted proteins
    1.
    发明授权
    Isolating cells expressing secreted proteins 有权
    分离表达分泌蛋白的细胞

    公开(公告)号：US06919183B2

    公开(公告)日：2005-07-19

    申请号：US10050279

    申请日：2002-01-16

    申请人： James P. Fandl ,  Neil Stahl ,  Gang Chen ,  George D. Yancopoulos

    发明人： James P. Fandl ,  Neil Stahl ,  Gang Chen ,  George D. Yancopoulos

    IPC分类号： A01K67/027 ,  C07K14/315 ,  C07K14/52 ,  C07K16/28 ,  C12N5/10 ,  C12N15/09 ,  C40B30/04 ,  G01N33/569 ,  G01N33/68 ,  G01N33/53 ,  C12N21/02 ,  C12N21/08

    CPC分类号： C40B30/04 ,  A01K2217/05 ,  A01K2267/01 ,  C07K14/315 ,  C07K14/52 ,  C07K16/2863 ,  C07K2317/77 ,  C07K2319/00 ,  C12N15/1051 ,  G01N33/56966 ,  G01N33/6845 ,  G01N2500/10

    摘要： A method for identifying and isolating cells which produce secreted proteins. This method is based upon a specific characteristic or the expression level of the secreted protein by transiently capturing the secreted protein on the surface of an individual cell, allowing selection of rare cell clones from a heterogeneous population. Also provided is the use of this method to generate cells which produce a desired level of secreted protein or secreted protein of a particular characteristic(s), and organisms which possess such cells. In particular, the method allows rapid isolation of high expression recombinant antibody-producing cell lines, or may be applied directly to rapid isolation of specific hybridomas, or to the isolation of antibody-producing transgenic animals. This method is applicable for any cell which secretes protein.

    摘要翻译： 用于鉴定和分离产生分泌蛋白的细胞的方法。 该方法基于通过瞬时捕获单个细胞表面上的分泌蛋白质的分泌蛋白质的特定特征或表达水平，允许从异质群体中选择稀有细胞克隆。 还提供了这种方法用于产生产生具有特定特征的所需水平的分泌蛋白质或分泌蛋白质的细胞以及具有这种细胞的生物体的用途。 特别地，该方法允许高表达重组抗体产生细胞系的快速分离，或者可以直接应用于快速分离特异性杂交瘤，或分离产生抗体的转基因动物。 该方法适用于分泌蛋白质的任何细胞。

    Isolating Cells Expressing Secreted Proteins
    2.
    发明申请
    Isolating Cells Expressing Secreted Proteins 审中-公开
    分离表达分泌蛋白的细胞

    公开(公告)号：US20090137416A1

    公开(公告)日：2009-05-28

    申请号：US12240541

    申请日：2008-09-29

    申请人： James P. Fandl ,  Gang Chen ,  Neil Stahl ,  George D. Yancopoulos

    发明人： James P. Fandl ,  Gang Chen ,  Neil Stahl ,  George D. Yancopoulos

    IPC分类号： C40B30/04 ,  G01N33/53

    CPC分类号： G01N33/6854 ,  C12N15/65 ,  G01N33/566 ,  G01N33/56966 ,  G01N2333/7051

    摘要： A method of detecting and isolating cells that produce a secreted protein of interest (POI) that has a T cell receptor variable domain, comprising: a) constructing a cell line transiently or stably expressing a cell surface capture molecule, which binds the POI, by transfecting the cell line with a nucleic acid that encodes such cell surface capture molecule; b) transfecting said cell simultaneously or subsequently with a second nucleic acid that encodes a POI wherein such POI is secreted; c) detecting the surface-displayed POI by contacting the cells with a detection molecule, which binds the POI; and d) isolating cells based on the detection molecule.

    摘要翻译： 一种检测和分离产生具有T细胞受体可变区的目标分泌蛋白质（POI）的细胞的方法，包括：a）通过以下步骤构建细胞系：瞬时或稳定表达结合POI的细胞表面捕获分子，通过 用编码这种细胞表面捕获分子的核酸转染细胞系; b）同时或随后用编码POI的第二核酸同时转染所述细胞，其中所述POI被分泌; c）通过使细胞与结合POI的检测分子接触来检测表面显示的POI; 和d）基于检测分子分离细胞。