公开(公告)号：US20080160096A1

公开(公告)日：2008-07-03

申请号：US11881831

申请日：2007-07-27

申请人： Janos Berbely ,  Magdolna Bodnar ,  Istvan Hajdu ,  John F. Hartmann ,  Zsolt Keresztessy ,  Laszlo Nagy ,  Gyorgy Vamosi

发明人： Janos Berbely ,  Magdolna Bodnar ,  Istvan Hajdu ,  John F. Hartmann ,  Zsolt Keresztessy ,  Laszlo Nagy ,  Gyorgy Vamosi

IPC分类号： A61K9/50

CPC分类号： A61K9/5138 ,  A61K9/5015 ,  A61K9/5026 ,  A61K9/5036 ,  A61K9/5146 ,  A61K9/5161 ,  A61K9/5192 ,  C12N15/87

摘要： The present invention relates to biocompatible and biodegradable stimuli-sensitive polymeric nanoparticles, which were formed by ion-ion interaction in aqueous media. Synthetic and biological macromolecules with ionizable functional groups are capable of forming nanoparticles whose size and surface properties are sensitive to environmental influences such as pH, temperature and salt concentration. Nanodevices are designed for therapeutic applications as drug and nucleic acid carriers, and/or for MRI diagnosis as contrast agents. These nanodevices are designed for therapeutic applications as targeted drug carriers. Additionally, they can be used as contrast agents for MRI diagnosis. These nanosystems are also potential carriers for delivery of active ingredients as DNA, RNA, short interfering RNA (siRNA), antisense oligonucleotides (AS-ON), and triple helix forming oligonucleotides (TFO) etc. for pharmaceutical applications. Their adjustable size offers yet another advantage.

摘要翻译： 本发明涉及生物相容性和生物可降解的刺激性敏感的聚合物纳米粒子，其通过在水性介质中的离子 - 离子相互作用而形成。 具有可离子化官能团的合成和生物大分子能够形成尺寸和表面性质对pH，温度和盐浓度等环境影响敏感的纳米颗粒。 纳米设备设计用作药物和核酸载体的治疗应用，和/或作为造影剂的MRI诊断。 这些纳米设备被设计用作靶向药物载体的治疗应用。 此外，它们可以用作MRI诊断的造影剂。 这些纳米系统也是作为药物应用的DNA，RNA，短干扰RNA（siRNA），反义寡核苷酸（AS-ON）和三螺旋形成寡核苷酸（TFO）等递送活性成分的潜在载体。 它们的可调节尺寸提供了另一个优点。

公开(公告)号：US20170175099A1

公开(公告)日：2017-06-22

申请号：US15381039

申请日：2016-12-15

申请人： Marton Szigeti ,  Akos Szekrenyes ,  Zsolt Keresztessy ,  Andras Guttman

发明人： Marton Szigeti ,  Akos Szekrenyes ,  Zsolt Keresztessy ,  Andras Guttman

IPC分类号： C12N11/10 ,  C12P19/04

CPC分类号： C12N11/10 ,  C07K2319/23 ,  C12N9/24 ,  C12P19/00 ,  C12P19/04 ,  C12P19/26 ,  C12P21/005 ,  C12Y302/02 ,  C12Y305/01052

摘要： N-glycosylation profiling of glycoprotein biotherapeutics is an essential step in each phase of product development in the biopharmaceutical industry. For example, during clone selection hundreds of clones should be analyzed quickly from limited amounts of samples. On the other hand, identification of disease related glycosylation alterations can serve as early indicators for various pathological conditions in the biomedical field. We describe an improved packed bed column PNGase F functionalized column reactor. The reactor may be packed into a pipette tip column. In some embodiments, a second column or mixed stationary phase may be packed into the column to capture and purify the cleaved glycan prior to analysis. Complete N-glycan removal can be obtained in 10 minutes from all major N-linked glycoprotein types. The approach can be readily applied to automated sample preparation systems, such as liquid handling robots.