公开(公告)号：US06448061B1

公开(公告)日：2002-09-10

申请号：US09463484

申请日：2000-01-27

申请人： Jao Go Pan ,  Soo An Shin ,  Chan Kyu Park ,  Pil Kim ,  Dong Eun Chang ,  Jae Eun Kim

发明人： Jao Go Pan ,  Soo An Shin ,  Chan Kyu Park ,  Pil Kim ,  Dong Eun Chang ,  Jae Eun Kim

IPC分类号： C12N120

CPC分类号： C12P7/46 ,  C12R1/19

摘要： This invention relates to a mutant Escherichia coli SS373 and the production of succinic acid by using the above strain. In detail, a novel E. coli SS373 (W3110 pta::Tn10 ldhA::Km) with the deficiency in the acetate and lactate forming pathways was constructed by genetic engineering technique. An aerobically grown SS373 was then cultured by means of the anaerobic condition shift during the succinate producing stage, which resulted in the efficient production of succinic acid with a higher yield.

摘要翻译： 本发明涉及突变型大肠杆菌SS373和通过使用上述菌株生产琥珀酸。 详细地，通过遗传工程技术构建了乙酸和乳酸盐形成途径缺乏的新型大肠杆菌SS373（W3110 pta :: Tn10 ldhA :: Km）。 然后在琥珀酸生产阶段通过厌氧条件转移培养有氧生长的SS373，从而以更高的产率有效生产琥珀酸。