公开(公告)号：US20220282294A1

公开(公告)日：2022-09-08

申请号：US17631407

申请日：2020-07-31

Applicant: Japan Science and Technology Agency

Inventor： Hiroshi Abe ,  Naoko Abe ,  Kosuke Nakamoto ,  Hiroki Murase ,  Yasuaki Kimura

IPC: C12P19/34 ,  C07H19/10 ,  C07H19/20

Abstract: A primer for amplifying a nucleic acid having a structure represented by the formula (1): where B represents a base, R1 represents a decomposable protecting group, and R2 represents hydrogen or a hydroxyl group, and the symbol * represents a bond to a sugar of an adjacent nucleotide. A device for producing double-stranded DNA, includes: a forward primer and a reverse primer, having a structure represented by formula (1); a PCR device for forming double-stranded DNA with 3′-recessed ends by performing multiple cycles of PCR by using a template DNA as a template; and a photoirradiation unit for deprotecting R1 and forming a sticky end with a 3′-protruding end.

公开(公告)号：US11597745B2

公开(公告)日：2023-03-07

申请号：US16977525

申请日：2019-03-08

Applicant: Japan Science and Technology Agency

Inventor： Hiroshi Abe ,  Yasuaki Kimura

IPC: C07H19/20 ,  C07H19/16

Abstract: A β-modified phosphoric acid compound precursor that inhibits the progress of a phosphorylation reaction having a partial structure represented by where A1 represents —SR1, —S—S—R1, —SeR1, or —X, where X is a halogen selected from fluoro, chloro, bromo, and iodo; R1 represents hydrogen, an alkyl group having 1 to 20 carbon atoms, or the like; L1 represents hydrogen, an alkyl group having 1 to 20 carbon atoms, or the like; L2 represents an alkyl group having 1 to 20 carbon atoms, or the like; L1 and L2 may be linked to each other to form a 4 to 6-membered ring structure; L1 and L2 may each have a substituent; and the symbol * represents a bond to be bonded to a phosphate group by phosphorylation. Further, provided are a reaction inhibitor and a medicine, each of which includes the β-modified phosphoric acid compound precursor.

公开(公告)号：US20210284679A1

公开(公告)日：2021-09-16

申请号：US16977525

申请日：2019-03-08

Applicant: Japan Science and Technology Agency

Inventor： Hiroshi Abe ,  Yasuaki Kimura

IPC: C07H19/20 ,  C07H19/16

Abstract: A β-modified phosphoric acid compound precursor having a partial structure represented by where A1 represents —SR1, —S—S—R1, —SeR1, or —X, where X is a halogen selected from fluorine, chlorine, bromine, and iodine; R1 represents hydrogen, an alkyl group having 1 to 20 carbon atoms, or the like; L1 represents hydrogen, an alkyl group having 1 to 20 carbon atoms, or the like; L2 represents an alkyl group having 1 to 20 carbon atoms, or the like; L1 and L2 may be linked to each other to form a 4 to 6-membered ring structure; L1 and L2 may each have a substituent; and the symbol * represents a bond to be bonded to a phosphate group by phosphorylation. Further, provided are a reaction inhibitor and a medicine, each of which includes the β-modified phosphoric acid compound precursor.

公开(公告)号：US20240327444A1

公开(公告)日：2024-10-03

申请号：US18576587

申请日：2022-07-05

Applicant: Japan Science and Technology Agency ,  National University Corporatjion Tokai National Higher Education and Research System

Inventor： Hiroshi Abe ,  Naoko Abe ,  Masahito Inagaki ,  Yasuaki Kimura ,  Fumitaka Hashiya ,  Zhenmin Li ,  Yuko Nakashima

IPC: C07H1/06 ,  A61K31/7105 ,  C07F9/24 ,  C07H17/02 ,  C07H21/00

CPC classification number: C07H1/06 ,  A61K31/7105 ,  C07F9/2458 ,  C07H17/02 ,  C07H21/00

Abstract: A method for purifying a nucleotide-based substance; including a protecting group introduction step of introducing a hydrophobic protecting group represented by the following formula (P1) or (P2) into a nucleotide-based substance to produce a hydrophobic nucleotide-based substance; an isolation and purification step of isolating and purifying the hydrophobic nucleotide-based substance under a hydrophobic environment; and a deprotection step of deprotecting the hydrophobic protecting group from the hydrophobic nucleotide-based substance to produce the nucleotide-based substance,






wherein R1 represents a linear or branched alkyl group having 1 to 30 carbon atoms, R4 represents hydrogen or a linear or branched alkyl group having 1 to 10 carbon atoms, R2, R3, R5 and R6 represent hydrogen, a linear or branched alkyl group having 1 to 10 carbon atoms, or the like, and may be the same or different; and * means a bond with a nucleotide-based substance.

公开(公告)号：US20230097172A1

公开(公告)日：2023-03-30

申请号：US17802680

申请日：2021-02-19

Applicant: Japan Science and Technology Agency

Inventor： Hiroshi Abe ,  Yasuaki Kimura ,  Naoko Abe

IPC: C07H21/02 ,  C12P19/34

Abstract: A method for producing a capped RNA which is an RNA having the 5′-end modified with a cap, the method including: reacting an activated capping compound represented by with a monophosphate RNA having the 5′-end monophosphorylated, where, L represents a leaving group. The activated capping compound is preferably a compound represented by

公开(公告)号：US20220290232A1

公开(公告)日：2022-09-15

申请号：US17631418

申请日：2020-07-31

Applicant: Japan Science and Technology Agency

Inventor： Hiroshi Abe ,  Naoko Abe ,  Kosuke Nakamoto ,  Hiroki Murase ,  Yasuaki Kimura

IPC: C12Q1/6876

Abstract: A primer for amplifying a nucleic acid having a structure represented by formula (1): where A1 represents —S—, —S—S—, or —Se—, B represents a base, and R1 represents a decomposable protecting group, and the symbol * represents a bond to a sugar of an adjacent nucleotide. A device for producing double-stranded DNA includes: a forward primer and a reverse primer, having a structure represented by formula (1); a PCR device for forming double-stranded DNA with 3′-recessed ends by performing multiple cycles of PCR by using a template DNA as a template; Klenow fragment for making the 3′ ends blunt; and a photoirradiation unit for deprotecting R1 and forming a sticky end with a 3′-protruding end.