公开(公告)号：US20100041033A1

公开(公告)日：2010-02-18

申请号：US12368949

申请日：2009-02-10

申请人： Jeffery F. Miller ,  Sergei Doulatov ,  Asher Hodes ,  Min Rennella ,  Mari Gingery ,  Huatao Guo ,  David W. Martin

发明人： Jeffery F. Miller ,  Sergei Doulatov ,  Asher Hodes ,  Min Rennella ,  Mari Gingery ,  Huatao Guo ,  David W. Martin

IPC分类号： C12Q1/68 ,  C12N15/31 ,  C12N1/21 ,  C12N15/74 ,  C40B40/06

CPC分类号： C12N15/102 ,  C12N15/1034 ,  C12N15/1058

摘要： This invention relates to the diversification of nucleic acid sequences by use of a nucleic acid molecule containing a region of sequence that acts as a template for diversification. The invention thus provides nucleic acid molecules to be diversified, as well as those which act as the template region (TR) and in concert with the TR for directional, site-specific diversification. Further provided are methods of preparing and using these nucleic acid sequences.

摘要翻译： 本发明涉及通过使用含有充当用于多样化的模板的序列区域的核酸分子来实现核酸序列的多样化。 因此，本发明提供要多样化的核酸分子，以及用作模板区域（TR）并与TR一起用于定向，位点特异性多样化的核酸分子。 还提供了制备和使用这些核酸序列的方法。

公开(公告)号：US07585957B2

公开(公告)日：2009-09-08

申请号：US11197219

申请日：2005-08-03

申请人： Jeffery F. Miller ,  Sergei Doulatov ,  Asher Hodes ,  Min Xu ,  Mari Gingery ,  David W. Martin, Jr.

发明人： Jeffery F. Miller ,  Sergei Doulatov ,  Asher Hodes ,  Min Xu ,  Mari Gingery ,  David W. Martin, Jr.

IPC分类号： C07H21/02 ,  C12N5/00 ,  C12N1/00 ,  C12N15/00 ,  C40B40/06

CPC分类号： C12N15/1024 ,  C12N15/102 ,  C12N15/1034 ,  C12N15/1058

摘要： This invention relates to the diversification of nucleic acid sequences by use of a nucleic acid molecule containing a region of sequence that acts as a template for diversification. The invention thus provides nucleic acid molecules to be diversified, as well as those which act as the template region (TR) and in concert with the TR for directional, site-specific diversification. Further provided are methods of preparing and using these nucleic acid sequences.

摘要翻译： 本发明涉及通过使用含有充当用于多样化的模板的序列区域的核酸分子来实现核酸序列的多样化。 因此，本发明提供要多样化的核酸分子，以及用作模板区域（TR）并与TR一起用于定向，位点特异性多样化的核酸分子。 还提供了制备和使用这些核酸序列的方法。

公开(公告)号：US20060121450A1

公开(公告)日：2006-06-08

申请号：US11197219

申请日：2005-08-03

申请人： Jeffery Miller ,  Sergei Doulatov ,  Asher Hodes ,  Min Xu ,  Mari Gingery ,  David Martin

发明人： Jeffery Miller ,  Sergei Doulatov ,  Asher Hodes ,  Min Xu ,  Mari Gingery ,  David Martin

IPC分类号： C40B40/08 ,  C40B40/10

CPC分类号： C12N15/1024 ,  C12N15/102 ,  C12N15/1034 ,  C12N15/1058

摘要： This invention relates to the diversification of nucleic acid sequences by use of a nucleic acid molecule containing a region of sequence that acts as a template for diversification. The invention thus provides nucleic acid molecules to be diversified, as well as those which act as the template region (TR) and in concert with the TR for directional, site-specific diversification. Further provided are methods of preparing and using these nucleic acid sequences.

公开(公告)号：US20120252060A1

公开(公告)日：2012-10-04

申请号：US13500186

申请日：2010-10-08

申请人： John Dick ,  Faiyaz Notta ,  Sergei Doulatov ,  Elisa Laurenti

发明人： John Dick ,  Faiyaz Notta ,  Sergei Doulatov ,  Elisa Laurenti

IPC分类号： C12N5/0789 ,  G01N21/64 ,  C12Q1/04

CPC分类号： C12N5/0647

摘要： This invention relates to human hematopoietic stem cells. Specifically the invention relations to the identification of single human hematopoietic stem cells capable of long-term multilineage engraftment and self-renewal. The invention also relates to an early lymphoid progenitor with monocytic potential, including dendritic cell potential.

摘要翻译： 本发明涉及人类造血干细胞。 具体地说，本发明与鉴定能够长期多系植入和自我更新的单人造血干细胞的关系。 本发明还涉及具有单核细胞电位的早期淋巴样祖细胞，包括树突状细胞电位。