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    Cellulase enzyme mixtures for depilling and uses thereof
    3.
    发明授权
    Cellulase enzyme mixtures for depilling and uses thereof 有权
    用于脱胶的纤维素酶混合物及其用途

    公开(公告)号：US09238806B2

    公开(公告)日：2016-01-19

    申请号：US13981198

    申请日：2012-02-09

    申请人： Christopher Hill ,  John J. Tomashek ,  Loreta Gudynaite-Savitch ,  Guylaine Therrien ,  Sophie Calixte

    发明人： Christopher Hill ,  John J. Tomashek ,  Loreta Gudynaite-Savitch ,  Guylaine Therrien ,  Sophie Calixte

    IPC分类号： C12N9/42 ,  C12N1/00 ,  D06M16/00 ,  C07K1/00 ,  C07H21/04

    CPC分类号： C12N9/2437 ,  C12Y302/01004 ,  C12Y302/01091 ,  D06M16/00 ,  D06M16/003

    摘要： The present invention relates to a depilling composition comprising an enzyme mixture that comprises a Family 45 cellulase and one or more additional cellulases selected from a Family 5 cellulase, a Family 6 cellulase or a combination thereof. The enzyme mixture may be characterized in that the Family 45 and Family 5 cellulases or the Family 45 and Family 6 cellulases are present at a weight ratio that exhibits synergy in an assay that measures specific depilling activity. The enzyme mixture may be secreted by a genetically modified microbe overexpressing the foregoing cellulases. Also provided is a process for depilling that comprises a step of contacting cellulose-containing goods with the depilling composition.

    摘要翻译： 本发明涉及一种脱毛组合物，其包含酶混合物，其包含家族45纤维素酶和一种或多种另外的选自家族5纤维素酶，家族6纤维素酶或其组合的纤维素酶。 该酶混合物的特征在于，45族和5族纤维素酶或45族和6族纤维素酶以测量特定脱盐活性的测定中显示协同作用的重量比存在。 酶混合物可以由过表达上述纤维素酶的遗传修饰的微生物分泌。 还提供了一种脱毛方法，其包括使含有纤维素的物品与脱毛剂组合物接触的步骤。

    Cellobiohydrolase enzymes
    4.
    发明授权
    Cellobiohydrolase enzymes 有权
    纤维二糖水解酶

    公开(公告)号：US09279163B2

    公开(公告)日：2016-03-08

    申请号：US13593735

    申请日：2012-08-24

    申请人： Jacqueline Montalibet ,  Loreta Gudynaite-Savitch ,  Christopher Hill ,  Christopher D. Hindle ,  James A. Lavigne ,  Nabil Masri ,  Fuad Tahna ,  John J. Tomashek

    发明人： Jacqueline Montalibet ,  Loreta Gudynaite-Savitch ,  Christopher Hill ,  Christopher D. Hindle ,  James A. Lavigne ,  Nabil Masri ,  Fuad Tahna ,  John J. Tomashek

    IPC分类号： C12N9/42 ,  C12N15/80 ,  C12P19/14

    CPC分类号： C12Y302/01091 ,  C12N9/2437 ,  C12N15/80 ,  C12P19/14

    摘要： Provided are isolated cellobiohydrolases comprising a modified Glycoside Hydrolase (GH) Family 7 catalytic domain, a GH Family 7 catalytic domain and a modified Family 1 carbohydrate binding module (CBM), or both a modified Family 7 catalytic domain and a modified Family 1 CBM. Such isolated cellobiohydrolases exhibit from 45% to about 99.9% amino acid sequence identity to amino acids 1-436 of SEQ ID NO: 1 or to amino acids 1-438 of SEQ ID NO: 2 and improved activity on process substrates. Also provided are genetic constructs and genetically modified microbes for expressing the isolated cellobiohydrolases, a process for producing the isolated cellobiohydrolases, cellulase enzyme mixtures comprising the isolated cellobiohydrolase and a process for hydrolyzing a cellulosic substrate with such cellulase enzyme mixtures.

    摘要翻译： 提供的是分离的纤维二糖水解酶，其包含修饰的糖苷水解酶（GH）家族7催化结构域，GH家族7催化结构域和修饰的家族1碳水化合物结合模块（CBM），或修饰的家族7催化结构域和修饰的家族1 CBM两者。 这种分离的纤维二糖水解酶显示与SEQ ID NO：1的氨基酸1-436或SEQ ID NO：2的氨基酸1-438具有45％至约99.9％的氨基酸序列同一性，并且改善了对工艺底物的活性。 还提供了用于表达分离的纤维二糖水解酶的遗传构建体和遗传修饰的微生物，用于产生分离的纤维二糖水解酶的方法，包含分离的纤维二糖水解酶的纤维素酶混合物和用这种纤维素酶混合物水解纤维素底物的方法。

    Hosts and fermentation processes for cellulase production
    5.
    发明授权
    Hosts and fermentation processes for cellulase production 有权
    用于纤维素酶生产的宿主和发酵过程

    公开(公告)号：US08323931B2

    公开(公告)日：2012-12-04

    申请号：US12611486

    申请日：2009-11-03

    申请人： Loreta Gudynaite-Savitch ,  Christopher D. Hindle ,  Theresa C. White

    发明人： Loreta Gudynaite-Savitch ,  Christopher D. Hindle ,  Theresa C. White

    IPC分类号： C12P19/14 ,  C12P21/06 ,  C12N9/42 ,  C12N1/00 ,  C08B37/00 ,  D21C1/00 ,  C02F3/34

    CPC分类号： C12N9/2437 ,  C12N1/38

    摘要： A fermentation process for the production of cellulase mixtures is provided. The process comprises providing a genetically modified host filamentous fungus that overexpresses a Xyr1 transcription factor and/or that is partially or completely deficient in expressing one or more hemicellulase enzyme. The host filamentous fungus is cultured in a medium comprising a carbon source. The carbon source contains from about 60 wt % to about 100 wt % hemicellulose-derived carbohydrate and less than 5% of a cellulase-inducing carbohydrate or contains from about 25 wt % to about 100% wt % hemicellulose-derived sugar alcohol in combination with from about 0 wt % to about 75 wt % glucose, glycerol or a combination thereof.

    摘要翻译： 提供了生产纤维素酶混合物的发酵方法。 该方法包括提供过表达Xyr1转录因子的遗传修饰宿主丝状真菌和/或部分或完全缺乏表达一种或多种半纤维素酶的基因。 宿主丝状真菌在包含碳源的培养基中培养。 碳源含有约60重量％至约100重量％的半纤维素衍生的碳水化合物和小于5％的纤维素酶诱导性碳水化合物，或含有约25重量％至约100重量％的半纤维素衍生的糖醇， 约0重量％至约75重量％的葡萄糖，甘油或其组合。

    NOVEL CELLOBIOHYDROLASE ENZYMES
    6.
    发明申请
    NOVEL CELLOBIOHYDROLASE ENZYMES 有权
    新型碳水化合物酶

    公开(公告)号：US20130052694A1

    公开(公告)日：2013-02-28

    申请号：US13593735

    申请日：2012-08-24

    申请人： Jacqueline Montalibet ,  Loreta Gudynaite-Savitch ,  Christopher Hill ,  Christopher D. Hindle ,  James A. Lavigne ,  Nabil Masri ,  Fuad Tahna ,  John J. Tomashek

    发明人： Jacqueline Montalibet ,  Loreta Gudynaite-Savitch ,  Christopher Hill ,  Christopher D. Hindle ,  James A. Lavigne ,  Nabil Masri ,  Fuad Tahna ,  John J. Tomashek

    IPC分类号： C12N9/42 ,  C12P19/14 ,  C12N1/19 ,  C12N1/21 ,  C12N15/63 ,  C12N1/15

    CPC分类号： C12Y302/01091 ,  C12N9/2437 ,  C12N15/80 ,  C12P19/14

    摘要： Provided are isolated cellobiohydrolases comprising a modified Glycoside Hydrolase (GH) Family 7 catalytic domain, a GH Family 7 catalytic domain and a modified Family 1 carbohydrate binding module (CBM), or both a modified Family 7 catalytic domain and a modified Family 1 CBM. Such isolated cellobiohydrolases exhibit from 45% to about 99.9% amino acid sequence identity to amino acids 1-436 of SEQ ID NO: 1 or to amino acids 1-438 of SEQ ID NO: 2 and improved activity on process substrates. Also provided are genetic constructs and genetically modified microbes for expressing the isolated cellobiohydrolases, a process for producing the isolated cellobiohydrolases, cellulase enzyme mixtures comprising the isolated cellobiohydrolase and a process for hydrolysing a cellulosic substrate with such cellulase enzyme mixtures.

    摘要翻译： 提供的是分离的纤维二糖水解酶，其包含修饰的糖苷水解酶（GH）家族7催化结构域，GH家族7催化结构域和修饰的家族1碳水化合物结合模块（CBM），或修饰的家族7催化结构域和修饰的家族1 CBM两者。 这种分离的纤维二糖水解酶显示与SEQ ID NO：1的氨基酸1-436或SEQ ID NO：2的氨基酸1-438具有45％至约99.9％的氨基酸序列同一性，并且改善了对工艺底物的活性。 还提供用于表达分离的纤维二糖水解酶的遗传构建体和遗传修饰的微生物，用于产生分离的纤维二糖水解酶的方法，包含分离的纤维二糖水解酶的纤维素酶混合物和用这种纤维素酶混合物水解纤维素底物的方法。

    Cellulase Enzyme Mixtures For Depilling and Uses Thereof
    7.
    发明申请
    Cellulase Enzyme Mixtures For Depilling and Uses Thereof 有权
    纤维素酶混合物用于其用途和用途

    公开(公告)号：US20130337542A1

    公开(公告)日：2013-12-19

    申请号：US13981198

    申请日：2012-02-09

    申请人： Christopher Hill ,  John J. Tomashek ,  Loreta Gudynaite-Savitch ,  Guylaine Therrien ,  Sophie Calixte

    发明人： Christopher Hill ,  John J. Tomashek ,  Loreta Gudynaite-Savitch ,  Guylaine Therrien ,  Sophie Calixte

    IPC分类号： C12N9/42

    CPC分类号： C12N9/2437 ,  C12Y302/01004 ,  C12Y302/01091 ,  D06M16/00 ,  D06M16/003

    摘要： The present invention relates to a depilling composition comprising an enzyme mixture that comprises a Family 45 cellulase enzyme component and one or more additional cellulase enzyme components selected from a Family 5 cellulase, a Family 6 cellulase or a combination thereof. The enzyme mixture may be characterized in that the Family 45 and the Family 5 cellulase enzyme components or the Family 45 and the Family 6 cellulase enzyme components are present at a weight ratio that exhibits synergy in an assay that measures specific depilling activity. The enzyme mixture may be secreted by a genetically modified microbe overexpressing theforegoing cellulase enzyme components. Also provided is a process for depilling that comprises a step of contacting cellulose-containing goods with the depilling composition.

    摘要翻译： 本发明涉及一种脱毛组合物，其包含含有家族45纤维素酶组分和选自家族5纤维素酶，家族6纤维素酶或其组合的一种或多种另外的纤维素酶组分的酶混合物。 该酶混合物的特征可以在于家族45和家族5纤维素酶组分或家族45和家族6纤维素酶组分以在测定特定去毛刺活性的测定中表现出协同作用的重量比存在。 酶混合物可以由过表达前述的纤维素酶组分的遗传修饰的微生物分泌。 还提供了一种脱毛方法，其包括使含有纤维素的物品与脱毛剂组合物接触的步骤。

    HOSTS AND FERMENTATION PROCESSES FOR CELLULASE PRODUCTION
    8.
    发明申请
    HOSTS AND FERMENTATION PROCESSES FOR CELLULASE PRODUCTION 有权
    用于生产细胞培养基的宿主和发酵过程

    公开(公告)号：US20100129880A1

    公开(公告)日：2010-05-27

    申请号：US12611486

    申请日：2009-11-03

    申请人： Loreta Gudynaite-Savitch ,  Christopher D. Hindle ,  Theresa C. White

    发明人： Loreta Gudynaite-Savitch ,  Christopher D. Hindle ,  Theresa C. White

    IPC分类号： C12P19/14 ,  C12N9/42

    CPC分类号： C12N9/2437 ,  C12N1/38

    摘要： A fermentation process for the production of cellulase mixtures is provided. The process comprises providing a genetically modified host filamentous fungus that overexpresses a Xyr1 transcription factor and/or that is partially or completely deficient in expressing one or more hemicellulase enzyme. The host filamentous fungus is cultured in a medium comprising a carbon source. The carbon source contains from about 60 wt % to about 100 wt % hemicellulose-derived carbohydrate and less than 5% of a cellulase-inducing carbohydrate or contains from about 25 wt % to about 100% wt % hemicellulose-derived sugar alcohol in combination with from about 0 wt % to about 75 wt % glucose, glycerol or a combination thereof.

    摘要翻译： 提供了生产纤维素酶混合物的发酵方法。 该方法包括提供过表达Xyr1转录因子的遗传修饰宿主丝状真菌和/或部分或完全缺乏表达一种或多种半纤维素酶的基因。 宿主丝状真菌在包含碳源的培养基中培养。 碳源含有约60重量％至约100重量％的半纤维素衍生的碳水化合物和小于5％的纤维素酶诱导性碳水化合物，或含有约25重量％至约100重量％的半纤维素衍生的糖醇， 约0重量％至约75重量％的葡萄糖，甘油或其组合。