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公开(公告)号：US09238806B2
公开(公告)日：2016-01-19
申请号：US13981198
申请日：2012-02-09
申请人： Christopher Hill , John J. Tomashek , Loreta Gudynaite-Savitch , Guylaine Therrien , Sophie Calixte
发明人： Christopher Hill , John J. Tomashek , Loreta Gudynaite-Savitch , Guylaine Therrien , Sophie Calixte
CPC分类号： C12N9/2437 , C12Y302/01004 , C12Y302/01091 , D06M16/00 , D06M16/003
摘要： The present invention relates to a depilling composition comprising an enzyme mixture that comprises a Family 45 cellulase and one or more additional cellulases selected from a Family 5 cellulase, a Family 6 cellulase or a combination thereof. The enzyme mixture may be characterized in that the Family 45 and Family 5 cellulases or the Family 45 and Family 6 cellulases are present at a weight ratio that exhibits synergy in an assay that measures specific depilling activity. The enzyme mixture may be secreted by a genetically modified microbe overexpressing the foregoing cellulases. Also provided is a process for depilling that comprises a step of contacting cellulose-containing goods with the depilling composition.
摘要翻译： 本发明涉及一种脱毛组合物，其包含酶混合物，其包含家族45纤维素酶和一种或多种另外的选自家族5纤维素酶，家族6纤维素酶或其组合的纤维素酶。 该酶混合物的特征在于，45族和5族纤维素酶或45族和6族纤维素酶以测量特定脱盐活性的测定中显示协同作用的重量比存在。 酶混合物可以由过表达上述纤维素酶的遗传修饰的微生物分泌。 还提供了一种脱毛方法，其包括使含有纤维素的物品与脱毛剂组合物接触的步骤。
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公开(公告)号：US20130337542A1
公开(公告)日：2013-12-19
申请号：US13981198
申请日：2012-02-09
申请人： Christopher Hill , John J. Tomashek , Loreta Gudynaite-Savitch , Guylaine Therrien , Sophie Calixte
发明人： Christopher Hill , John J. Tomashek , Loreta Gudynaite-Savitch , Guylaine Therrien , Sophie Calixte
IPC分类号： C12N9/42
CPC分类号： C12N9/2437 , C12Y302/01004 , C12Y302/01091 , D06M16/00 , D06M16/003
摘要： The present invention relates to a depilling composition comprising an enzyme mixture that comprises a Family 45 cellulase enzyme component and one or more additional cellulase enzyme components selected from a Family 5 cellulase, a Family 6 cellulase or a combination thereof. The enzyme mixture may be characterized in that the Family 45 and the Family 5 cellulase enzyme components or the Family 45 and the Family 6 cellulase enzyme components are present at a weight ratio that exhibits synergy in an assay that measures specific depilling activity. The enzyme mixture may be secreted by a genetically modified microbe overexpressing theforegoing cellulase enzyme components. Also provided is a process for depilling that comprises a step of contacting cellulose-containing goods with the depilling composition.
摘要翻译： 本发明涉及一种脱毛组合物，其包含含有家族45纤维素酶组分和选自家族5纤维素酶，家族6纤维素酶或其组合的一种或多种另外的纤维素酶组分的酶混合物。 该酶混合物的特征可以在于家族45和家族5纤维素酶组分或家族45和家族6纤维素酶组分以在测定特定去毛刺活性的测定中表现出协同作用的重量比存在。 酶混合物可以由过表达前述的纤维素酶组分的遗传修饰的微生物分泌。 还提供了一种脱毛方法，其包括使含有纤维素的物品与脱毛剂组合物接触的步骤。
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公开(公告)号：US09279163B2
公开(公告)日：2016-03-08
申请号：US13593735
申请日：2012-08-24
申请人： Jacqueline Montalibet , Loreta Gudynaite-Savitch , Christopher Hill , Christopher D. Hindle , James A. Lavigne , Nabil Masri , Fuad Tahna , John J. Tomashek
发明人： Jacqueline Montalibet , Loreta Gudynaite-Savitch , Christopher Hill , Christopher D. Hindle , James A. Lavigne , Nabil Masri , Fuad Tahna , John J. Tomashek
CPC分类号： C12Y302/01091 , C12N9/2437 , C12N15/80 , C12P19/14
摘要： Provided are isolated cellobiohydrolases comprising a modified Glycoside Hydrolase (GH) Family 7 catalytic domain, a GH Family 7 catalytic domain and a modified Family 1 carbohydrate binding module (CBM), or both a modified Family 7 catalytic domain and a modified Family 1 CBM. Such isolated cellobiohydrolases exhibit from 45% to about 99.9% amino acid sequence identity to amino acids 1-436 of SEQ ID NO: 1 or to amino acids 1-438 of SEQ ID NO: 2 and improved activity on process substrates. Also provided are genetic constructs and genetically modified microbes for expressing the isolated cellobiohydrolases, a process for producing the isolated cellobiohydrolases, cellulase enzyme mixtures comprising the isolated cellobiohydrolase and a process for hydrolyzing a cellulosic substrate with such cellulase enzyme mixtures.
摘要翻译： 提供的是分离的纤维二糖水解酶，其包含修饰的糖苷水解酶（GH）家族7催化结构域，GH家族7催化结构域和修饰的家族1碳水化合物结合模块（CBM），或修饰的家族7催化结构域和修饰的家族1 CBM两者。 这种分离的纤维二糖水解酶显示与SEQ ID NO：1的氨基酸1-436或SEQ ID NO：2的氨基酸1-438具有45％至约99.9％的氨基酸序列同一性，并且改善了对工艺底物的活性。 还提供了用于表达分离的纤维二糖水解酶的遗传构建体和遗传修饰的微生物，用于产生分离的纤维二糖水解酶的方法，包含分离的纤维二糖水解酶的纤维素酶混合物和用这种纤维素酶混合物水解纤维素底物的方法。
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公开(公告)号：US20130052694A1
公开(公告)日：2013-02-28
申请号：US13593735
申请日：2012-08-24
申请人： Jacqueline Montalibet , Loreta Gudynaite-Savitch , Christopher Hill , Christopher D. Hindle , James A. Lavigne , Nabil Masri , Fuad Tahna , John J. Tomashek
发明人： Jacqueline Montalibet , Loreta Gudynaite-Savitch , Christopher Hill , Christopher D. Hindle , James A. Lavigne , Nabil Masri , Fuad Tahna , John J. Tomashek
CPC分类号： C12Y302/01091 , C12N9/2437 , C12N15/80 , C12P19/14
摘要： Provided are isolated cellobiohydrolases comprising a modified Glycoside Hydrolase (GH) Family 7 catalytic domain, a GH Family 7 catalytic domain and a modified Family 1 carbohydrate binding module (CBM), or both a modified Family 7 catalytic domain and a modified Family 1 CBM. Such isolated cellobiohydrolases exhibit from 45% to about 99.9% amino acid sequence identity to amino acids 1-436 of SEQ ID NO: 1 or to amino acids 1-438 of SEQ ID NO: 2 and improved activity on process substrates. Also provided are genetic constructs and genetically modified microbes for expressing the isolated cellobiohydrolases, a process for producing the isolated cellobiohydrolases, cellulase enzyme mixtures comprising the isolated cellobiohydrolase and a process for hydrolysing a cellulosic substrate with such cellulase enzyme mixtures.
摘要翻译： 提供的是分离的纤维二糖水解酶，其包含修饰的糖苷水解酶（GH）家族7催化结构域，GH家族7催化结构域和修饰的家族1碳水化合物结合模块（CBM），或修饰的家族7催化结构域和修饰的家族1 CBM两者。 这种分离的纤维二糖水解酶显示与SEQ ID NO：1的氨基酸1-436或SEQ ID NO：2的氨基酸1-438具有45％至约99.9％的氨基酸序列同一性，并且改善了对工艺底物的活性。 还提供用于表达分离的纤维二糖水解酶的遗传构建体和遗传修饰的微生物，用于产生分离的纤维二糖水解酶的方法，包含分离的纤维二糖水解酶的纤维素酶混合物和用这种纤维素酶混合物水解纤维素底物的方法。
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公开(公告)号：US20100093040A1
公开(公告)日：2010-04-15
申请号：US12550615
申请日：2009-08-31
CPC分类号： C12P19/14 , C12N9/2445 , C12Y302/01021
摘要： Provided are modified beta-glucosidase enzymes, derived from the Trichoderma reesei Cel3A beta-glucosidase, that exhibit improved stability at low pH, low pH and high aeration, low pH and high agitation, or low pH and elevated temperature. Also provided are genetic constructs comprising nucleotide sequences encoding for modified beta-glucosidase enzymes, methods for the production of modified beta-glucosidase enzymes from host strains and the use of the modified beta-glucosidase enzymes in the hydrolysis of cellulose.
摘要翻译： 提供了源自里氏木霉Cel3Aβ-葡糖苷酶的修饰的β-葡糖苷酶，其在低pH，低pH和高通气，低pH和高搅拌，或低pH和升高的温度下表现出改善的稳定性。 还提供了包含编码修饰的β-葡糖苷酶的核苷酸序列的遗传构建体，用于从宿主菌株产生修饰的β-葡糖苷酶的方法和在纤维素水解中使用经修饰的β-葡糖苷酶。
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公开(公告)号：US08143049B2
公开(公告)日：2012-03-27
申请号：US12550615
申请日：2009-08-31
CPC分类号： C12P19/14 , C12N9/2445 , C12Y302/01021
摘要： Provided are modified beta-glucosidase enzymes, derived from the Trichoderma reesei Cel3A beta-glucosidase, that exhibit improved stability at low pH, low pH and high aeration, low pH and high agitation, or low pH and elevated temperature. Also provided are genetic constructs comprising nucleotide sequences encoding for modified beta-glucosidase enzymes, methods for the production of modified beta-glucosidase enzymes from host strains and the use of the modified beta-glucosidase enzymes in the hydrolysis of cellulose.
摘要翻译： 提供了源自里氏木霉Cel3Aβ-葡糖苷酶的修饰的β-葡糖苷酶，其在低pH，低pH和高通气，低pH和高搅拌，或低pH和升高的温度下表现出改善的稳定性。 还提供了包含编码修饰的β-葡糖苷酶的核苷酸序列的遗传构建体，用于从宿主菌株产生修饰的β-葡糖苷酶的方法和在纤维素水解中使用经修饰的β-葡糖苷酶。
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7.发明授权公开(公告)号：US09279112B2
公开(公告)日：2016-03-08
申请号：US12713516
申请日：2010-02-26
申请人： Brian R. Scott , Patrick St-Pierre , James Lavigne , Nabil Masri , Theresa C. White , John J. Tomashek
发明人： Brian R. Scott , Patrick St-Pierre , James Lavigne , Nabil Masri , Theresa C. White , John J. Tomashek
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14
摘要： Provided are modified cellulase enzymes exhibiting increase cellulose-hydrolyzing activity in the presence of lignin and/or reduced binding to lignin comprising modified linker peptides comprising one or more amino acid substitutions, insertions, or deletions that result in (a) a decrease in the calculated isoelectric point of the linker peptide and/or (b) an increase in the ratio of threonine:serine in the linker peptide relative to a parental linker peptide from which said modified linker peptide is derived. Also provided are genetic constructs comprising nucleic acid sequences encoding for modified cellulase enzymes, methods for the production of the modified cellulase enzymes from host strains and a process for hydrolyzing cellulose with the modified cellulases in the presence of lignin.
摘要翻译： 提供了在木质素存在下表现出增加的纤维素水解活性的修饰的纤维素酶和/或包含修饰的连接肽的修饰的连接肽，其包含一个或多个氨基酸取代，插入或缺失，导致（a）计算的 接头肽的等电点和/或（b）相对于衍生了所述修饰的接头肽的亲本接头肽，接头肽中苏氨酸：丝氨酸的比例增加。 还提供了包含编码修饰的纤维素酶的核酸序列的遗传构建体，从宿主菌株制备修饰的纤维素酶的方法和在木质素存在下用修饰的纤维素酶水解纤维素的方法。
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公开(公告)号：US20100041100A1
公开(公告)日：2010-02-18
申请号：US12512403
申请日：2009-07-30
CPC分类号： C12N9/2437
摘要： A modified Trichoderma reesei Family 6 (TrCel6A) cellulase enzyme comprising amino acid substitutions at one or more positions selected from the group consisting of 129, 322, 363 and 410 of SEQ ID NO: 1 is provided. Genetic constructs and genetically modified microbes comprising nucleic sequences encoding the modified TrCel6a cellulase are also provided. The modified TrCel6A cellulase of the invention display at least a 15% decrease in inactivation by lignin relative to a parental TrCel6A cellulase from which the modified TrCel6A is derived. Such cellulases find use in a variety of applications in industry requiring enzymatic hydrolysis of cellulose in the presence of lignin, e.g., the hydrolysis of pretreated lignocellulosic feedstocks for the production of fermentable sugars, sugar alcohols and fuel alcohols.
摘要翻译： 提供了修饰的里氏木霉家族6（TrCel6A）纤维素酶，其在选自SEQ ID NO：1的129,322,363和410的一个或多个位置上包含氨基酸取代。 还提供了包含编码修饰的TrCel6a纤维素酶的核酸序列的遗传构建体和遗传修饰微生物。 本发明的经修饰的TrCel6A纤维素酶相对于来自其的TrCel6A衍生的亲本TrCel6A纤维素酶显示出至少15％的木质素灭活降低。 这种纤维素酶可用于需要在木质素存在下酶促水解纤维素的工业中的各种应用，例如用于生产可发酵糖，糖醇和燃料醇的预处理的木质纤维素原料的水解。
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公开(公告)号：US20120295308A1
公开(公告)日：2012-11-22
申请号：US13575046
申请日：2011-02-11
IPC分类号： C07K14/37 , C12N15/63 , C12N1/19 , C12P7/02 , C12N1/15 , C12P21/02 , C12P19/14 , C12N9/42 , C12N1/21
CPC分类号： C12N9/2437 , C07K2319/20 , C12N9/2402 , C12N9/2445 , C12P7/06 , C12P19/02 , C12P19/14 , C12Y302/01004 , C12Y302/01021 , Y02E50/16 , Y02E50/17
摘要： Provided is a modified Family 1 carbohydrate binding module (CBM) comprising amino acid substitutions at one or more of positions 10, 11, 12, 14, 17, 21, 24, 29, 31, 33, and 37, said position determined from alignment of a Family 1 CBM amino acid sequence with SEQ ID NO: 30, and exhibiting from about 50% to about 99.9% amino acid sequence identity to SEQ ID NO: 30. Also provided are modified glycosidase enzymes comprising the modified Family 1 CBM, genetic constructs and genetically modified microbes for expressing the modified Family 1 CBM or modified glycosidase enzyme. The modified Family 1 CBM confers reduced lignin binding and/or increased hydrolysing activity in the presence of lignin to the modified glycosidase enzyme, which may be used in a process for hydrolysing cellulose or hemicellulose in the presence of lignin.
摘要翻译： 提供了一种修饰的1族碳水化合物结合模块（CBM），其包含位置10,11,12,14,17,21,24,29,31,33和37中的一个或多个的氨基酸取代，所述位置由对准确定 具有SEQ ID NO：30的家族1 CBM氨基酸序列，并且与SEQ ID NO：30显示约50％至约99.9％的氨基酸序列同一性。还提供了包含修饰的家族1 CBM，遗传 构建体和用于表达经修饰的家族1 CBM或修饰的糖苷酶的转基因微生物。 修饰的家族1 CBM在木质素存在下赋予木质素结合和/或增加的水解活性至修饰的糖苷酶，其可用于在木质素存在下水解纤维素或半纤维素的方法。
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公开(公告)号：US20100221778A1
公开(公告)日：2010-09-02
申请号：US12713516
申请日：2010-02-26
申请人： Brian R. Scott , Patrick St-Pierre , James Lavigne , Nabil Masri , Theresa C. White , John J. Tomashek
发明人： Brian R. Scott , Patrick St-Pierre , James Lavigne , Nabil Masri , Theresa C. White , John J. Tomashek
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14
摘要： Provided are modified cellulase enzymes exhibiting increase cellulose-hydrolyzing activity in the presence of lignin and/or reduced binding to lignin comprising modified linker peptides comprising one or more amino acid substitutions, insertions, or deletions that result in (a) a decrease in the calculated isoelectric point of the linker peptide and/or (b) an increase in the ratio of threonine:serine in the linker peptide relative to a parental linker peptide from which said modified linker peptide is derived. Also provided are genetic constructs comprising nucleic acidsequences encoding for modified cellulase enzymes, methods for the production of the modified cellulase enzymes from host strains and a process for hydrolysing cellulose with the modified cellulases in the presence of lignin.
摘要翻译： 提供了在木质素存在下表现出增加的纤维素水解活性的修饰的纤维素酶和/或包含修饰的连接肽的修饰的连接肽，其包含一个或多个氨基酸取代，插入或缺失，导致（a）计算的 接头肽的等电点和/或（b）相对于衍生了所述修饰的接头肽的亲本接头肽，接头肽中苏氨酸：丝氨酸的比例增加。 还提供了包含编码修饰的纤维素酶的核酸序列的遗传构建体，从宿主菌株生产修饰的纤维素酶的方法和在木质素存在下用修饰的纤维素酶水解纤维素的方法。
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