公开(公告)号：US20200032191A1

公开(公告)日：2020-01-30

申请号：US16586989

申请日：2019-09-29

申请人： Jian Chen

发明人： Jian Chen

IPC分类号： C12M1/42 ,  C12M1/12 ,  B01L3/00

摘要： The shock tube comprises a tube, a first electrode, a second electrode and a stopple, wherein the tube is internally provided with a cavity for accommodating a target liquid sample. The first electrode is arranged at one end of the tube. The second electrode is arranged in the stopple, and the outer end of the second electrode can be electrically connected with the exterior via an opening of the stopple. The stopple is internally provided with an elastic piece connected with the second electrode. The outer side of the elastic piece is connected with the stopple, and the inner side of the elastic piece is connected with the second electrode. The invention further provides a cell electroporation device where the shock tube can be placed.

公开(公告)号：US10392634B2

公开(公告)日：2019-08-27

申请号：US15472003

申请日：2017-03-28

申请人： Jingwen Zhou ,  Jian Chen ,  Yongkun Lv ,  Guocheng Du

发明人： Jingwen Zhou ,  Jian Chen ,  Yongkun Lv ,  Guocheng Du

IPC分类号： C12P7/22 ,  C12N9/04 ,  C12N9/08 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04 ,  C12N15/62 ,  C12P17/18

摘要： The present invention provides a method for high-efficiency production of pinoresinol by use of an H2O2 auto-scavenging enzymatic cascade. It uses eugenol as the substrate, which is relatively inexpensive and is industrially available. It uses an enzymatic cascade to remove H2O2 produced in the process of pinoresinol synthesis, thereby reducing its inhibitory effect on the enzyme activity. In addition, the present invention uses whole cells as a catalyst, which can continuously regenerate cofactors needed by the enzyme, thus eliminating the need for exogenous addition of expensive cofactors during the reaction. The yield of the present invention can reach 7.12 g/L and the conversion rate is 61.55%.

公开(公告)号：US10085470B2

公开(公告)日：2018-10-02

申请号：US15204991

申请日：2016-07-07

申请人： Fang Fang ,  Jiran Zhang ,  Xifei Yang ,  Chuanwang Hu ,  Jian Chen ,  Guocheng Du

发明人： Fang Fang ,  Jiran Zhang ,  Xifei Yang ,  Chuanwang Hu ,  Jian Chen ,  Guocheng Du

IPC分类号： A23L5/20 ,  A23L27/50 ,  A23L23/00 ,  C12N1/20 ,  C12R1/07

摘要： The present invention provides a method for improving the quality of soy sauce using Bacillus amyloliquefaciens, which relates to the field of microbiology and food technology. The method comprises inoculating Bacillus amyloliquefaciens BBE JY06 during soy sauce fermentation. With Bacillus amyloliquefaciens addition during soy sauce fermentation, the aroma and tastes of soy sauce is improved and the ethyl carbamate content in soy sauce is decreased as well.

公开(公告)号：US09690855B2

公开(公告)日：2017-06-27

申请号：US13549416

申请日：2012-07-14

申请人： Sheng Hua Bao ,  Jian Chen ,  Zhong Su ,  Rui Wang

发明人： Sheng Hua Bao ,  Jian Chen ,  Zhong Su ,  Rui Wang

IPC分类号： G06F17/30

CPC分类号： G06F17/30864

摘要： Provided is a method applied to a system including a plurality of sensors, a proxy, and a server. The method includes: measuring objects by the plurality of sensors to obtain first measurement values; computing a correlation among the first measurement values by the server based on the first measurement values; computing an actual measurement value of a second measurement value by the proxy based on the first measurement values and a given function; verifying the plurality of sensors by setting one or more sensors as verification target sensors in turn by the server at predetermined timing; computing a predicted value of the second measurement value by the server based on the correlation and the first measurement values obtained from the other sensors; and outputting the predicted value of the second measurement value instead of the actual measurement value thereof at least during the verification of the verification target sensors.

公开(公告)号：US09601991B2

公开(公告)日：2017-03-21

申请号：US13438398

申请日：2012-04-03

申请人： Jian Chen

发明人： Jian Chen

IPC分类号： H02M3/335 ,  H02M1/42

CPC分类号： H02M1/4258 ,  Y02B70/126

摘要： In a switching regulator control circuit according to aspects of the invention, a drain current is converted to a voltage Vis with a resistance. The voltage is delivered to a multiplication circuit. The multiplication circuit generates and outputs a voltage that is a product signal of the voltage and a voltage that is proportional to a duty factor. A comparator circuit compares the voltage with an error signal delivered to the other comparison input terminal of the comparator. When the voltage has reached the error voltage, the comparator delivers a turn-off instruction through an OR circuit to a terminal of a flip-flop.

公开(公告)号：US09390134B2

公开(公告)日：2016-07-12

申请号：US13339043

申请日：2011-12-28

申请人： Rui Hu ,  Jian Chen

发明人： Rui Hu ,  Jian Chen

IPC分类号： G06F17/30

CPC分类号： G06F17/30516 ,  G06F17/30867

摘要： A regular expression matching method and system, and a searching device are provided. First, the searching device performs string filtering on a data stream to be matched, in which if keywords in the data stream and preset character words have at least one same character, the searching device indicates that the data stream passes through the string filtering. Then the searching device performs regular expression filtering on the data stream passing through the string filtering. In a string filtering process through the method, system, and device, when Hash mapping positions of the keywords of the data stream are a subset of the Hash mapping positions of the character words, it indicates that the data stream passes through the string filtering, and it is not required to store the keywords and further compare the keywords with the character words, thereby saving the storage space and improving performance.

摘要翻译： 提供了正则表达式匹配方法和系统，以及搜索装置。 首先，搜索装置对要匹配的数据流执行字符串过滤，其中如果数据流中的关键字和预设字符字具有至少一个相同的字符，则搜索装置指示数据流通过字符串过滤。 然后，搜索设备对通过字符串过滤的数据流执行正则表达式过滤。 在通过方法，系统和设备的字符串过滤过程中，当数据流的关键字的哈希映射位置是字符字的哈希映射位置的子集时，表示数据流通过字符串过滤， 并且不需要存储关键词并进一步将关键词与字符词进行比较，从而节省存储空间并提高性能。

公开(公告)号：US09328363B2

公开(公告)日：2016-05-03

申请号：US14142897

申请日：2013-12-29

申请人： Jian Chen ,  Jingwen Zhou ,  Hongwei Guo ,  Guocheng Du

发明人： Jian Chen ,  Jingwen Zhou ,  Hongwei Guo ,  Guocheng Du

IPC分类号： C12N15/74 ,  C12N1/20 ,  C12P7/50 ,  C07K14/39 ,  C12N15/81 ,  C12N1/16

CPC分类号： C12P7/50 ,  C07K14/39 ,  C12N1/16 ,  C12N15/815

摘要： The present invention provides four keto acid transporter encoding sequences selected from 6611 protein coding sequences of Yarrowia lipolytica CLIB122 database. Also provided are recombinant Yarrowia lipolytica strains overexpressing the keto acid transporters, which have increased extracellular secretion of α-ketoglutarate. The present invention can be used to increase extracellular levels of α-ketoglutarate during the fermentation process and lower downstream purification cost for α-ketoglutarate production.

摘要翻译： 本发明提供了选自解脂耶氏酵母CLIB122数据库的6611个蛋白质编码序列的四个酮酸转运蛋白编码序列。 还提供了过表达酮酸转运蛋白的重组耶氏酵母解脂耶氏酵母菌株，其增加了α-酮戊二酸的细胞外分泌。 本发明可用于增加发酵过程中α-酮戊二酸的细胞外水平，降低α-酮戊二酸生产的下游净化成本。

公开(公告)号：US20150343452A1

公开(公告)日：2015-12-03

申请号：US14289325

申请日：2014-05-28

申请人： Licheng Zhou ,  Jian Chen ,  Jianbo Qi ,  Haiting Chen ,  Ziqiang Ding

发明人： Licheng Zhou ,  Jian Chen ,  Jianbo Qi ,  Haiting Chen ,  Ziqiang Ding

IPC分类号： B02C18/22 ,  B65H5/00 ,  B02C18/00

CPC分类号： B02C18/2275 ,  B02C18/0007 ,  B02C18/24 ,  B02C2018/164 ,  B02C2018/2208 ,  B65H5/00

摘要： A document shredder is disclosed including a paper receptacle for receiving a load of paper and a shredding assembly. A metering assembly is interposed between the paper receptacle and the shredding assembly and is configured to separate a portion of the load of paper from the load of paper and permit the portion of the load of paper to move into the shredding assembly. The metering assembly may include a stop plate secured thereto and defining a lifting portion on a perimeter portion thereof. The lifting portion is configured to engage a lower edge of the portion of the load of paper upon rotation of the stop plate and lift the paper over the stop plate. The lifting portion may include a flange, which may be a portion of a cone, formed on a perimeter portion of the wheel and occupying a sector of the perimeter of less than 60 degrees.

摘要翻译： 公开了一种文件粉碎机，其包括用于接收纸张和切碎组件的纸容器。 计量组件插入在纸容器和切碎组件之间，并且构造成将纸张的一部分与纸张的负载分开并且允许纸张的一部分移动到粉碎组件中。 计量组件可以包括固定到其上的限位板，并在其周边部分上限定提升部分。 提升部分构造成在止动板旋转时接合纸张载荷部分的下边缘并将纸提起在止动板上。 提升部分可以包括形成在轮的周边部分上并且占据小于60度的周长的扇形部的凸缘，其可以是锥体的一部分。

公开(公告)号：US20150104794A1

公开(公告)日：2015-04-16

申请号：US14142897

申请日：2013-12-29

申请人： Jian Chen ,  Jingwen Zhou ,  Hongwei Guo ,  Guocheng Du

发明人： Jian Chen ,  Jingwen Zhou ,  Hongwei Guo ,  Guocheng Du

IPC分类号： C12P7/50 ,  C12Q1/68

CPC分类号： C12P7/50 ,  C07K14/39 ,  C12N1/16 ,  C12N15/815

摘要： The present invention provides four keto acid transporter encoding sequences selected from 6611 protein coding sequences of Yarrowia lipolytica CLIB122 database. Also provided are recombinant Yarrowia lipolytica strains overexpressing the keto acid transporters, which have increased extracellular secretion of α-ketoglutarate. The present invention can be used to increase extracellular levels of α-ketoglutarate during the fermentation process and lower downstream purification cost for α-ketoglutarate production.

摘要翻译： 本发明提供了选自解脂耶氏酵母CLIB122数据库的6611个蛋白质编码序列的四个酮酸转运蛋白编码序列。 还提供了过表达酮酸转运蛋白的重组耶氏酵母解脂耶氏酵母菌株，其增加了α-酮戊二酸的细胞外分泌。 本发明可用于增加发酵过程中α-酮戊二酸的细胞外水平，降低α-酮戊二酸生产的下游净化成本。

公开(公告)号：US08878506B2

公开(公告)日：2014-11-04

申请号：US13695860

申请日：2012-02-20

申请人： Jian Chen

发明人： Jian Chen

IPC分类号： G06F1/00 ,  G05F1/10 ,  H02M1/42 ,  H02M1/12

CPC分类号： G05F1/10 ,  H02M1/12 ,  H02M1/4225 ,  Y02B70/126

摘要： An object of the present invention is to achieve the reduction of EMI, the improvement of conversion efficiency, and simplification of the input filter. Provided is a switching power supply device that comprises: a chopper circuit that turns on/off the passage of a current to an inductor by means of a switching element; and a switching control circuit that switches the switching element so that an input voltage and an input current have the same phase and a similar shape. The switching control circuit is configured to switch the switching element at a switching frequency that is proportional to an input voltage dependent term of a formula that expresses a third harmonic component of ripples of the current flowing to the inductor.

摘要翻译： 本发明的目的是实现EMI的降低，转换效率的提高和输入滤波器的简化。 提供了一种开关电源装置，其包括：斩波电路，其通过开关元件接通/断开电流通过电感器; 以及开关控制电路，其切换开关元件，使得输入电压和输入电流具有相同的相位和相似的形状。 开关控制电路被配置为以与表示流向电感器的电流的波纹的三次谐波分量的公式的输入电压相关项成比例的开关频率来切换开关元件。