公开(公告)号：US20230078437A1

公开(公告)日：2023-03-16

申请号：US17990793

申请日：2022-11-21

Applicant: Jiangnan University

Inventor： Bo JIANG ,  Ying PAN ,  Tao ZHANG ,  Jingjing CHEN

IPC: A23C9/156 ,  A23L33/00 ,  A23C9/158 ,  A23C9/152 ,  A23C1/04 ,  A23L33/115 ,  A23L33/125 ,  A23L33/16 ,  A23L33/155 ,  A23L33/19

Abstract: The present disclosure discloses a milk powder containing maltopentaose trehalose instead of maltodextrin and a preparation method thereof, and belongs to the field of processing of dairy products. In the present disclosure, a formula milk powder containing maltopentaose trehalose instead of maltodextrin includes the following raw materials in parts by mass: 85-90 parts of fresh milk, 1-2 parts of maltopentaose trehalose, 5-10 parts of vegetable oil, 1-2 parts of a desalted whey powder, 1.5-3 parts of a concentrated whey protein powder, 0.5-0.8 parts of lactose, 0.05-0.1 parts of a multivitamin, and 0.05-0.1 parts of a complex mineral. Differences in physical and chemical properties of products due to a nonuniform polymerization degree of maltodextrin are reduced, and the problem that a Maillard reaction is likely to be induced when the maltodextrin is mixed with an amino acid or protein is solved.

公开(公告)号：US20210403894A1

公开(公告)日：2021-12-30

申请号：US17463628

申请日：2021-09-01

Applicant: Jiangnan University

Inventor： Bo JIANG ,  Tao ZHANG ,  Qing MENG ,  Jingjing CHEN

IPC: C12N9/88 ,  C12N1/20

Abstract: The disclosure discloses an alginate lyase and application thereof, and belongs to the technical field of biology. The alginate lyase provided by the disclosure has high degradation activity, and the enzyme activity reaches 65 U/mg; the alginate lyase is stable in nature, and the enzyme activity remains 98% or higher of the initial enzyme activity after storage at 4° C. for 18 months; and the alginate lyase has high product specificity. The disclosure uses E. coli as a host to express the alginate lyase derived from V. natriegens, the obtained recombinant E. coli can produce the alginate lyase secreted extracellularly in a conventional LB medium without adding an induction substrate sodium alginate, so the downstream processing technology of protein is simplified, and the disclosure has great industrial application potential.