公开(公告)号：US07816580B2

公开(公告)日：2010-10-19

申请号：US12722981

申请日：2010-03-12

申请人： Jianru Zuo ,  Qi-Wen Niu ,  Giovanna Frugis ,  Nam-Hai Chua

发明人： Jianru Zuo ,  Qi-Wen Niu ,  Giovanna Frugis ,  Nam-Hai Chua

IPC分类号： C12N15/82

CPC分类号： C12N15/8261 ,  A01H4/005 ,  C12N15/821 ,  C12N15/8287 ,  Y02A40/146

摘要： The present invention relates to methods for promoting somatic embryogenesis from a tissue or organ of a plant, by overexpressing a Wuschel gene in said tissue or organ. In one embodiment, such overexpression can be used as a silent selectable marker for transgenic plants. In another embodiment, such expression can be used to confer apomixis to a plant. In another embodiment, such overexpression can be used to create haploid plants, which can be used to produce dihaploid plants.

摘要翻译： 本发明涉及通过过表达所述组织或器官中的Wuschel基因来促进来自植物的组织或器官的体细胞胚胎发生的方法。 在一个实施方案中，这种过表达可以用作转基因植物的沉默选择标记。 在另一个实施方案中，这种表达可以用于赋予植物无义变异体。 在另一个实施方案中，这种过表达可用于产生单倍体植物，其可用于产生双倍体植物。

公开(公告)号：US08431773B2

公开(公告)日：2013-04-30

申请号：US13347839

申请日：2012-01-11

申请人： Jianru Zuo ,  Qi-Wen Niu ,  Giovanna Frugis ,  Nam-Hai Chua

发明人： Jianru Zuo ,  Qi-Wen Niu ,  Giovanna Frugis ,  Nam-Hai Chua

IPC分类号： C12N15/82 ,  A01H1/00

CPC分类号： C12N15/8261 ,  A01H4/005 ,  C12N15/821 ,  C12N15/8287 ,  Y02A40/146

摘要： The present invention relates to methods for promoting somatic embryogenesis from a tissue or organ of a plant, by overexpressing a Wuschel gene in said tissue or organ. In one embodiment, such overexpression can be used as a silent selectable marker for transgenic plants. In another embodiment, such expression can be used to confer apomixis to a plant. In another embodiment, such overexpression can be used to create haploid plants, which can be used to produce dihaploid plants.

公开(公告)号：US08101821B2

公开(公告)日：2012-01-24

申请号：US13164904

申请日：2011-06-21

申请人： Jianru Zuo ,  Qi-Wen Niu ,  Giovanna Frugis ,  Nam-Hai Chua

发明人： Jianru Zuo ,  Qi-Wen Niu ,  Giovanna Frugis ,  Nam-Hai Chua

IPC分类号： C12N15/82

CPC分类号： C12N15/8261 ,  A01H4/005 ,  C12N15/821 ,  C12N15/8287 ,  Y02A40/146

摘要： The present invention relates to methods for promoting somatic embryogenesis from a tissue or organ of a plant, by overexpressing a Wuschel gene in said tissue or organ. In one embodiment, such overexpression can be used as a silent selectable marker for transgenic plants. In another embodiment, such expression can be used to confer apomixis to a plant. In another embodiment, such overexpression can be used to create haploid plants, which can be used to produce dihaploid plants.

摘要翻译： 本发明涉及通过过表达所述组织或器官中的Wuschel基因来促进来自植物的组织或器官的体细胞胚胎发生的方法。 在一个实施方案中，这种过表达可以用作转基因植物的沉默选择标记。 在另一个实施方案中，这种表达可以用于赋予植物无义变异体。 在另一个实施方案中，这种过表达可用于产生单倍体植物，其可用于产生双倍体植物。

公开(公告)号：US07977534B2

公开(公告)日：2011-07-12

申请号：US12888636

申请日：2010-09-23

申请人： Jianru Zuo ,  Qi-Wen Niu ,  Giovanna Frugis ,  Nam-Hai Chua

发明人： Jianru Zuo ,  Qi-Wen Niu ,  Giovanna Frugis ,  Nam-Hai Chua

IPC分类号： C12N15/82

CPC分类号： C12N15/8261 ,  A01H4/005 ,  C12N15/821 ,  C12N15/8287 ,  Y02A40/146

摘要： The present invention relates to methods for promoting somatic embryogenesis from a tissue or organ of a plant, by overexpressing a Wuschel gene in said tissue or organ. In one embodiment, such overexpression can be used as a silent selectable marker for transgenic plants. In another embodiment, such expression can be used to confer apomixis to a plant. In another embodiment, such overexpression can be used to create haploid plants, which can be used to produce dihaploid plants.

摘要翻译： 本发明涉及通过过表达所述组织或器官中的Wuschel基因来促进来自植物的组织或器官的体细胞胚胎发生的方法。 在一个实施方案中，这种过表达可以用作转基因植物的沉默选择标记。 在另一个实施方案中，这种表达可以用于赋予植物无义变异体。 在另一个实施方案中，这种过表达可用于产生单倍体植物，其可用于产生双倍体植物。

公开(公告)号：US07700829B2

公开(公告)日：2010-04-20

申请号：US10956120

申请日：2004-10-04

申请人： Jianru Zuo ,  Qi-Wen Niu ,  Giovanna Frugis ,  Nam-Hai Chua

发明人： Jianru Zuo ,  Qi-Wen Niu ,  Giovanna Frugis ,  Nam-Hai Chua

IPC分类号： C12N15/82

CPC分类号： C12N15/8261 ,  A01H4/005 ,  C12N15/821 ,  C12N15/8287 ,  Y02A40/146

摘要： The present invention relates to methods for promoting somatic embryogenesis from a tissue or organ of a plant, by overexpressing a Wuschel gene in said tissue or organ. In one embodiment, such overexpression can be used as a silent selectable marker for transgenic plants. In another embodiment, such expression can be used to confer apomixis to a plant. In another embodiment, such overexpression can be used to create haploid plants, which can be used to produce dihaploid plants.

摘要翻译： 本发明涉及通过过表达所述组织或器官中的Wuschel基因来促进来自植物的组织或器官的体细胞胚胎发生的方法。 在一个实施方案中，这种过表达可以用作转基因植物的沉默选择标记。 在另一个实施方案中，这种表达可以用于赋予植物无义变异体。 在另一个实施方案中，这种过表达可用于产生单倍体植物，其可用于产生双倍体植物。

公开(公告)号：US20050071898A1

公开(公告)日：2005-03-31

申请号：US10956120

申请日：2004-10-04

申请人： Jianru Zuo ,  Qi-Wen Niu ,  Giovanna Frugis ,  Nam-Hai Chua

发明人： Jianru Zuo ,  Qi-Wen Niu ,  Giovanna Frugis ,  Nam-Hai Chua

IPC分类号： C12N15/82 ,  A01H1/00 ,  A01H5/00

CPC分类号： C12N15/8261 ,  A01H4/005 ,  C12N15/821 ,  C12N15/8287 ,  Y02A40/146

摘要： The present invention relates to methods for promoting somatic embryogenesis from a tissue or organ of a plant, by overexpressing a Wuschel gene in said tissue or organ. In one embodiment, such overexpression can be used as a silent selectable marker for transgenic plants. In another embodiment, such expression can be used to confer apomixis to a plant. In another embodiment, such overexpression can be used to create haploid plants, which can be used to produce dihaploid plants.

摘要翻译： 本发明涉及通过过表达所述组织或器官中的Wuschel基因来促进来自植物的组织或器官的体细胞胚胎发生的方法。 在一个实施方案中，这种过表达可以用作转基因植物的沉默选择标记。 在另一个实施方案中，这种表达可以用于赋予植物无义变异体。 在另一个实施方案中，这种过表达可用于产生单倍体植物，其可用于产生双倍体植物。

公开(公告)号：US06784340B1

公开(公告)日：2004-08-31

申请号：US09438392

申请日：1999-11-12

申请人： Takashi Aoyama ,  Jianru Zuo ,  Nam-Hai Chua

发明人： Takashi Aoyama ,  Jianru Zuo ,  Nam-Hai Chua

IPC分类号： A01H100

CPC分类号： C12N15/8274 ,  C12N15/8209 ,  C12N15/8237 ,  C12N15/8238 ,  C12N15/8242 ,  C12N15/8295

摘要： A chemically inducible promoter is described that may be used to transform plants, including tobacco and lettuce, with genes which are easily regulatable by adding the plants or plant cells to a medium containing an inducer of the promoter or by removing the plants or plant cells from such medium. The promoter described is one that is inducible by a glucocorticoid which is not endogenous to plants. Such promoters may be used with a variety of genes such as ipt or knotted1 to induce shoot formation in the presence of a glucocorticoid. The promoter may also be used with antibiotic or herbicide resistance genes which are then regulatable by the presence or absence of inducer rather than being constitutive. Other examples of genes which may be placed under the control of the inducible promoter are also presented.

摘要翻译： 描述了一种化学诱导型启动子，其可用于通过将植物或植物细胞添加到含有启动子诱导物的培养基的培养基中或通过从植物或植物细胞中除去而使其易于调节的基因转化植物，包括烟草和莴苣 这样的媒介。 所描述的启动子是可由植物内源性糖皮质激素诱导的启动子。 这样的启动子可以与各种基因如ipt或knotted1一起使用以在糖皮质激素存在下诱导芽形成。 启动子也可以与抗生素或除草剂抗性基因一起使用，然后通过存在或不存在诱导物而不是组成型的基因。 还可以列举可置于诱导型启动子控制下的基因的其他实例。

公开(公告)号：US06452068B1

公开(公告)日：2002-09-17

申请号：US09439535

申请日：1999-11-12

申请人： Jianru Zuo ,  Qiwen Niu ,  Nam-Hai Chua

发明人： Jianru Zuo ,  Qiwen Niu ,  Nam-Hai Chua

IPC分类号： C12N1582

CPC分类号： C12N15/8295 ,  C12N15/8209 ,  C12N15/821 ,  C12N15/8238 ,  C12N15/8242

摘要： Chemically inducible promoters are described that may be used to transform plants, including tobacco and lettuce, with genes which are easily regulatable by adding the plants or plant cells to a medium containing an inducer of the promoter or by removing the plants or plant cells from such medium. The promoters described are ones that are inducible by a glucocorticoid or estrogen which is not endogenous to plants. Such promoters may be used with a variety of genes such as ipt, CKI1, or knotted1, to induce shoot formation in the presence of an appropriate inducer. The promoters may be used with genes which induce somatic embryos such as Lec1 or SERK to prepare somatic embryos which can be grown into seedlings and then into plants. The promoter may also be used with antibiotic or herbicide resistance genes which are then regulatable by the presence or absence of inducer rather than being constitutive. Other examples of genes which may be placed under the control of the inducible promoter are also presented.

摘要翻译： 描述了可用于通过将植物或植物细胞添加到含有启动子诱导剂的培养基的培养基中或通过从其中除去植物或植物细胞而易于调节的基因来转化植物（包括烟草和莴苣）的化学诱导型启动子 中。 所描述的启动子是由糖皮质激素或雌激素诱导的不是植物内源性的启动子。 这样的启动子可以与各种基因如ipt，CKI1或打结的1一起使用，以在合适的诱导物存在下诱导芽形成。 启动子可以与诱导体细胞胚如Lec1或SERK的基因一起使用，以制备体细胞胚，其可以生长成幼苗，然后进入植物。 启动子也可以与抗生素或除草剂抗性基因一起使用，然后通过存在或不存在诱导物而不是组成型的基因。 还可以列举可置于诱导型启动子控制下的基因的其他实例。

公开(公告)号：US07148402B2

公开(公告)日：2006-12-12

申请号：US10850465

申请日：2004-05-21

申请人： Qi-Wen Niu ,  Nam-Hai Chua

发明人： Qi-Wen Niu ,  Nam-Hai Chua

IPC分类号： C12N15/82

CPC分类号： C12N15/8261 ,  A01H1/08 ,  A01H4/005 ,  A01H4/008 ,  C07K14/415 ,  C12N15/821 ,  C12N15/8287 ,  Y02A40/146

摘要： The present invention relates to methods for promoting somatic embryogenesis from a plant cell, tissue, organ, callus or cell culture, by overexpressing a PGA37 gene in the tissue or organ. In one embodiment, such overexpression can be used as a silent selectable marker for transgenic plants. In another embodiment, such expression can be used to confer apomixis to a plant. In another embodiment, such overexpression can be used to create haploid plants, which can be used to produce dihaploid plants.

摘要翻译： 本发明涉及通过过表达组织或器官中的PGA37基因来促进植物细胞，组织，器官，愈伤组织或细胞培养物中体细胞胚发生的方法。 在一个实施方案中，这种过表达可以用作转基因植物的沉默选择标记。 在另一个实施方案中，这种表达可以用于赋予植物无义变异体。 在另一个实施方案中，这种过表达可用于产生单倍体植物，其可用于产生双倍体植物。

公开(公告)号：US07525012B2

公开(公告)日：2009-04-28

申请号：US10196416

申请日：2002-07-17

申请人： Jianru Zuo ,  Qiwen Niu ,  Nam-Hai Chua

发明人： Jianru Zuo ,  Qiwen Niu ,  Nam-Hai Chua

IPC分类号： C12N15/82 ,  C12N5/00 ,  C12N15/63

CPC分类号： C12N15/8295 ,  C12N15/8209 ,  C12N15/821 ,  C12N15/8238 ,  C12N15/8242

摘要： Chemically inducible promoters are described that may be used to transform plants, including tobacco and lettuce, with genes which are easily regulatable by adding the plants or plant cells to a medium containing an inducer of the promoter or by removing the plants or plant cells from such medium. The promoters described are ones that are inducible by a glucocorticoid or estrogen which is not endogenous to plants. Such promoters may be used with a variety of genes such as ipt, CKI1, or knotted1, to induce shoot formation in the presence of an appropriate inducer. The promoters may be used with genes which induce somatic embryos such as Lec1 or SERK to prepare somatic embryos which can be grown into seedlings and then into plants. The promoter may also be used with antibiotic or herbicide resistance genes which are then regulatable by the presence or absence of inducer rather than being constitutive. Other examples of genes which may be placed under the control of the inducible promoter are also presented.