公开(公告)号：US20060134674A1

公开(公告)日：2006-06-22

申请号：US11295225

申请日：2005-12-05

申请人： Jing Huang ,  Keith Jones ,  Michael Shapero

发明人： Jing Huang ,  Keith Jones ,  Michael Shapero

IPC分类号： C12Q1/68 ,  G06F19/00

CPC分类号： G06F19/18 ,  C12Q1/6827 ,  C12Q1/6837 ,  G06F19/20 ,  C12Q2545/101

摘要： Methods of identifying allele-specific changes in genomic DNA copy number are disclosed. Methods for identifying homozygous deletions and genetic amplifications are disclosed. An array of probes designed to detect presence or absence of a plurality of different sequences is also disclosed. The probes are designed to hybridize to sequences that are predicted to be present in a reduced complexity sample. The methods may be used to detect copy number changes in cancerous tissue compared to normal tissue. The methods may be used to diagnose cancer and other diseases associated with chromosomal anomalies.

摘要翻译： 公开了确定基因组DNA拷贝数中等位基因特异性变化的方法。 公开了鉴定纯合缺失和遗传扩增的方法。 还公开了一种设计用于检测多个不同序列的存在或不存在的探针阵列。 探针被设计为与预测存在于复杂度降低的样品中的序列杂交。 与正常组织相比，该方法可用于检测癌组织中的拷贝数变化。 该方法可用于诊断与染色体异常相关的癌症和其他疾病。

公开(公告)号：US20050064476A1

公开(公告)日：2005-03-24

申请号：US10913928

申请日：2004-08-06

申请人： Jing Huang ,  Michael Shapero ,  Keith Jones ,  Guoying Liu

发明人： Jing Huang ,  Michael Shapero ,  Keith Jones ,  Guoying Liu

IPC分类号： C12Q20060101 ,  C12Q1/00 ,  C12Q1/68 ,  G01N33/48 ,  G01N33/50 ,  G06F19/18 ,  G06F19/20 ,  G06F19/00

CPC分类号： C12Q1/6837 ,  C12Q1/6827 ,  G16B20/00 ,  G16B25/00 ,  C12Q2545/101

摘要： Methods of identifying changes in genomic DNA copy number are disclosed. Methods for identifying homozygous deletions and genetic amplifications are disclosed. An array of probes designed to detect presence or absence of a plurality of different sequences is also disclosed. The probes are designed to hybridize to sequences that are predicted to be present in a reduced complexity sample. The methods may be used to detect copy number changes in cancerous tissue compared to normal tissue. The methods may be used to diagnose cancer and other diseases associated with chromosomal anomalies.

摘要翻译： 公开了鉴定基因组DNA拷贝数变化的方法。 公开了鉴定纯合缺失和遗传扩增的方法。 还公开了一种设计用于检测多个不同序列的存在或不存在的探针阵列。 探针被设计为与预测存在于复杂度降低的样品中的序列杂交。 与正常组织相比，该方法可用于检测癌组织中的拷贝数变化。 该方法可用于诊断与染色体异常相关的癌症和其他疾病。

公开(公告)号：US20050130217A1

公开(公告)日：2005-06-16

申请号：US11044831

申请日：2005-01-26

申请人： Jing Huang ,  Michael Shapero ,  Keith Jones ,  Guoying Liu

发明人： Jing Huang ,  Michael Shapero ,  Keith Jones ,  Guoying Liu

IPC分类号： C12Q20060101 ,  C12Q1/00 ,  C12Q1/68 ,  G01N33/48 ,  G01N33/50 ,  G06F19/18 ,  G06F19/20 ,  G06F19/00

CPC分类号： C12Q1/6837 ,  C12Q1/6827 ,  G16B20/00 ,  G16B25/00 ,  C12Q2545/101

摘要： Methods of identifying changes in genomic DNA copy number are disclosed. Methods for identifying homozygous deletions and genetic amplifications are disclosed. An array of probes designed to detect presence or absence of a plurality of different sequences is also disclosed. The probes are designed to hybridize to sequences that are predicted to be present in a reduced complexity sample. The methods may be used to detect copy number changes in cancerous tissue compared to normal tissue. The methods may be used to diagnose cancer and other diseases associated with chromosomal anomalies.

公开(公告)号：US20070128647A1

公开(公告)日：2007-06-07

申请号：US11608233

申请日：2006-12-07

申请人： Jing Huang ,  Keith Jones

发明人： Jing Huang ,  Keith Jones

IPC分类号： C12Q1/68 ,  G06F19/00

CPC分类号： G06F19/18 ,  C12Q2600/156

摘要： Methods for genotyping polymorphisms using allele specific probes are disclosed. A training set is used to generate a model for each polymorphism to be interrogated. The training set is used to obtain an estimate of the asymmetry between an intensity measurement for a first allele and an intensity measurement for a second allele of the same polymorphism. The intensity measurement obtained for a test sample is adjusted using the estimate of asymmetry prior to using the intensity measurements to make a genotyping call. In preferred embodiments the adjustment is applied to polymorphisms that have a likelihood of being heterozygous that is above a specified threshold.

摘要翻译： 公开了使用等位基因特异性探针进行基因分型多态性的方法。 训练集用于生成要查询的每个多态性的模型。 训练集用于获得第一等位基因的强度测量与相同多态性的第二等位基因的强度测量之间的不对称性的估计。 在使用强度测量进行基因分型调用之前，使用不对称估计来调整测试样品获得的强度测量值。 在优选实施方案中，调整适用于具有高于特定阈值的杂合可能性的多态性。

公开(公告)号：US09358249B2

公开(公告)日：2016-06-07

申请号：US13129539

申请日：2009-11-18

申请人： Jing Huang ,  Rui Hao ,  Brett Eugene Lomenick ,  Nao Jonai ,  Simon Diep

发明人： Jing Huang ,  Rui Hao ,  Brett Eugene Lomenick ,  Nao Jonai ,  Simon Diep

IPC分类号： A61K31/425 ,  A61K31/7105

CPC分类号： A61K31/7105 ,  G01N2333/4706 ,  G01N2500/10

摘要： In certain embodiments this invention pertains to the discovery that inhibition of the expression and/or activity of eukaryotic initiation factor 4A (eIF4A) inhibits the aging process. Accordingly, in certain embodiments, methods are provided for inhibiting/slowing aging. The methods typically involve administering to a mammal an agent that inhibits the expression and/or activity of eukaryotic initiation factor 4A (eIF4A) in an amount sufficient to inhibit expression or activity of EIF4A, where the agent is not resveratrol.

摘要翻译： 在某些实施方案中，本发明涉及抑制真核起始因子4A（eIF4A）的表达和/或活性抑制老化过程的发现。 因此，在某些实施方案中，提供了用于抑制/减缓老化的方法。 所述方法通常包括向哺乳动物施用以足以抑制EIF4A的表达或活性的量来抑制真核起始因子4A（eIF4A）的表达和/或活性的试剂，其中所述试剂不是白藜芦醇。

公开(公告)号：US20110251798A1

公开(公告)日：2011-10-13

申请号：US13167264

申请日：2011-06-23

申请人： Jing Huang ,  Keith W. Jones

发明人： Jing Huang ,  Keith W. Jones

IPC分类号： G06F19/00 ,  C40B60/12

CPC分类号： G06F19/18 ,  C12Q2600/156

摘要： Methods for genotyping polymorphisms using allele specific probes are disclosed. A training set is used to generate a model for each polymorphism to be interrogated. The training set is used to obtain an estimate of the asymmetry between an intensity measurement for a first allele and an intensity measurement for a second allele of the same polymorphism. The intensity measurement obtained for a test sample is adjusted using the estimate of asymmetry prior to using the intensity measurements to make a genotyping call. In preferred embodiments the adjustment is applied to polymorphisms that have a likelihood of being heterozygous that is above a specified threshold.

摘要翻译： 公开了使用等位基因特异性探针进行基因分型多态性的方法。 训练集用于生成要查询的每个多态性的模型。 训练集用于获得第一等位基因的强度测量与相同多态性的第二等位基因的强度测量之间的不对称性的估计。 在使用强度测量进行基因分型调用之前，使用不对称估计来调整测试样品获得的强度测量值。 在优选实施方案中，调整适用于具有高于特定阈值的杂合可能性的多态性。

公开(公告)号：US20110029251A1

公开(公告)日：2011-02-03

申请号：US12902711

申请日：2010-10-12

申请人： Jing Huang ,  Keith W. Jones ,  Michael H. Shapero

发明人： Jing Huang ,  Keith W. Jones ,  Michael H. Shapero

IPC分类号： G06F19/00 ,  G06F17/18

CPC分类号： G06F19/18 ,  C12Q1/6827 ,  C12Q1/6837 ,  G06F19/20 ,  C12Q2545/101

摘要： Methods of identifying allele-specific changes in genomic DNA copy number are disclosed. Methods for identifying homozygous deletions and genetic amplifications are disclosed. An array of probes designed to detect presence or absence of a plurality of different sequences is also disclosed. The probes are designed to hybridize to sequences that are predicted to be present in a reduced complexity sample. The methods may be used to detect copy number changes in cancerous tissue compared to normal tissue. The methods may be used to diagnose cancer and other diseases associated with chromosomal anomalies.

摘要翻译： 公开了确定基因组DNA拷贝数中等位基因特异性变化的方法。 公开了鉴定纯合缺失和遗传扩增的方法。 还公开了一种设计用于检测多个不同序列的存在或不存在的探针阵列。 探针被设计为与预测存在于复杂度降低的样品中的序列杂交。 与正常组织相比，该方法可用于检测癌组织中的拷贝数变化。 该方法可用于诊断与染色体异常相关的癌症和其他疾病。

公开(公告)号：US20100212003A1

公开(公告)日：2010-08-19

申请号：US12370665

申请日：2009-02-13

申请人： Franck Barillaud ,  Richard P. Christianson ,  Jing Huang ,  Richard Martin Scott ,  Joe Wesley Sutton, JR.

发明人： Franck Barillaud ,  Richard P. Christianson ,  Jing Huang ,  Richard Martin Scott ,  Joe Wesley Sutton, JR.

IPC分类号： H04L9/32

CPC分类号： G06Q20/382 ,  G06F21/6263 ,  G06Q20/12 ,  H04L63/0428 ,  H04L63/102 ,  H04L2463/102

摘要： A method, programmed medium and system are provided for implementing a prebuilt and encrypted personal identification information (PII) profile which resides only on a user's computer and is prevented from being permanently stored in a server's database. In an exemplary embodiment, when a user visits a web site and creates a new account, the site submits a request to query the user's profile using an extension to the HTTP protocol. The user is prompted by the user's browser to grant the site permission to do so and the site automatically uploads a non-personal identifying number (ID) to the user's system to create an account. All personal information remains on the user's computer within the user's encrypted PII profile and is not allowed to be stored in the server's storage. Therefore, each time the user purchases something, the site must again request to query the user's profile for the user's name, credit card information and/or billing address or other information, rather than keeping that information in the web site's datastore.

摘要翻译： 提供了一种方法，编程介质和系统，用于实现只存在于用户计算机上并被阻止永久存储在服务器数据库中的预建和加密的个人识别信息（PII）简档。 在示例性实施例中，当用户访问网站并创建新帐户时，网站使用HTTP协议的扩展来提交查询用户简档的请求。 由用户浏览器提示用户授予网站权限，网站会自动将非个人识别号码（ID）上传到用户系统以创建帐户。 所有个人信息保留在用户的加密PII配置文件中的用户计算机上，不允许存储在服务器的存储中。 因此，每当用户购买某物时，网站必须再次请求查询用户的个人资料以获取用户的姓名，信用卡信息和/或帐单地址或其他信息，而不是将该信息保留在网站的数据存储区中。

公开(公告)号：US20080201727A1

公开(公告)日：2008-08-21

申请号：US12107721

申请日：2008-04-22

申请人： Jeff Byers ,  Jing Huang ,  Khosrow Panah

发明人： Jeff Byers ,  Jing Huang ,  Khosrow Panah

IPC分类号： G06F9/54

CPC分类号： G06F9/4411

摘要： A method and apparatus for performing driver configuration operations without a system reboot is disclosed. In one embodiment, a network server's adapter driver receives a request to change a configuration of a selected instance of a plurality of instances. In response, the adapter driver may then determine if there is data flow through the selected instance. If there is no data flow through the selected instance, the method includes blocking subsequent data flow and subsequent information requests issued to the adapter driver. The selected instance may then be reinitializing without rebooting the server.

摘要翻译： 公开了一种在没有系统重启的情况下执行驱动程序配置操作的方法和装置。 在一个实施例中，网络服务器的适配器驱动程序接收改变多个实例的选定实例的配置的请求。 作为响应，适配器驱动器然后可以确定是否存在经过所选实例的数据流。 如果没有通过所选实例的数据流，则该方法包括阻止后续数据流和发送给适配器驱动程序的后续信息请求。 所选实例可能会重新初始化，而无需重新启动服务器。

公开(公告)号：US09280771B2

公开(公告)日：2016-03-08

申请号：US12370665

申请日：2009-02-13

申请人： Franck Barillaud ,  Richard P. Christianson ,  Jing Huang ,  Richard Martin Scott ,  Joe Wesley Sutton, Jr.

发明人： Franck Barillaud ,  Richard P. Christianson ,  Jing Huang ,  Richard Martin Scott ,  Joe Wesley Sutton, Jr.

IPC分类号： G06F17/30 ,  G06Q40/00 ,  G06Q20/38 ,  G06F21/62 ,  G06Q20/12 ,  H04L29/06

CPC分类号： G06Q20/382 ,  G06F21/6263 ,  G06Q20/12 ,  H04L63/0428 ,  H04L63/102 ,  H04L2463/102

摘要： A method, programmed medium and system are provided for implementing a prebuilt and encrypted personal identification information (PII) profile which resides only on a user's computer and is prevented from being permanently stored in a server's database. When a user visits a web site and creates a new account, the site submits a request to query the user's profile using an extension to the HTTP protocol. The user is prompted by the user's browser to grant the site permission to do so and the site automatically uploads a non-personal identifying number (ID) to the user's system to create an account. User-selected fields of the PII are transmitted to the server for processing a user-requested transaction. All personal information remains on the user's computer within the user's encrypted PII profile and is deleted at the server after the completion of the requested transaction.

摘要翻译： 提供了一种方法，编程介质和系统，用于实现只存在于用户计算机上并被阻止永久存储在服务器数据库中的预建和加密的个人识别信息（PII）简档。 当用户访问网站并创建新帐户时，网站会使用HTTP协议的扩展名来提交查询用户个人资料的请求。 由用户浏览器提示用户授予网站权限，网站会自动将非个人识别号码（ID）上传到用户系统以创建帐户。 将PII的用户选择的字段发送到服务器以处理用户请求的交易。 所有个人信息都保留在用户计算机的用户加密PII配置文件中，并在完成请求的交易后在服务器上删除。