公开(公告)号：US20190032062A1

公开(公告)日：2019-01-31

申请号：US15891564

申请日：2018-02-08

申请人： Jing Wu ,  Quan Zhang ,  Liming Liu ,  Xiulai Chen ,  Jia Liu ,  Qiuling Luo

发明人： Jing Wu ,  Quan Zhang ,  Liming Liu ,  Xiulai Chen ,  Jia Liu ,  Qiuling Luo

IPC分类号： C12N15/70 ,  C12P19/26 ,  C12N9/90 ,  C12N9/10 ,  C12P19/24

CPC分类号： C12N15/70 ,  C12N9/1096 ,  C12N9/90 ,  C12P19/24 ,  C12P19/26 ,  C12Y504/0201

摘要： The present invention relates to the field of biotechnology engineering. It provides a recombinant Escherichia coli that can produce fructosylated chondroitin with high yields and the method for constructing the recombinant strain. The present invention discloses a method for constructing an expression plasmid pETM6R1-RBS-glmM-GGGS-glmS that contains a glmM and a glmS gene under regulation of ribosome binding sites. The recombinant plasmid was transformed into E. coli K4 to obtain a recombinant E. coli strain ZQ33 that can produce fructosylated chondroitin up to 3.99 g·L−1 by fed-batch fermentation in a 5-L fermentor, which was increased by 108.90% compared to that of the wild type strain.

公开(公告)号：US20240191270A1

公开(公告)日：2024-06-13

申请号：US18555611

申请日：2022-04-15

申请人： INBIOSE N.V.

发明人： Joeri Beauprez ,  Pieter Coussement ,  Thomas Decoene

IPC分类号： C12P19/12 ,  C12N9/00 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12P19/26

CPC分类号： C12P19/12 ,  C12N9/0006 ,  C12N9/1025 ,  C12N9/1048 ,  C12N9/1051 ,  C12N9/1085 ,  C12N9/1205 ,  C12N9/1241 ,  C12N9/88 ,  C12N9/90 ,  C12N9/93 ,  C12N15/52 ,  C12P19/26 ,  C12Y101/01271 ,  C12Y203/03009 ,  C12Y204/01038 ,  C12Y204/01069 ,  C12Y204/01152 ,  C12Y207/01006 ,  C12Y207/01052 ,  C12Y207/07023 ,  C12Y207/0703 ,  C12Y207/07043 ,  C12Y401/03001 ,  C12Y402/01047 ,  C12Y504/02008 ,  C12Y504/0201 ,  C12Y602/01001

摘要： The present invention is in the technical field of synthetic biology and metabolic engineering. More particularly, the present invention is in the technical field of metabolically engineered cells and use of said cell in a cultivation, preferably a fermentation. The present invention describes a cell for the production of a compound. The cell comprises a pathway for the production of the compound, which can be a disaccharide, oligosaccharide and/or a Neu(n)Ac-containing bioproduct, wherein (n) is 4, 5, 7, 8 or 9 or a combination thereof. The cell is metabolically engineered for enhanced synthesis of acetyl-Coenzyme A. The invention also resides in a method of producing such compound by cultivation, preferably a fermentation, with such a cell.

公开(公告)号：US09771607B2

公开(公告)日：2017-09-26

申请号：US15011509

申请日：2016-01-30

申请人： Jian Chen ,  Zhen Kang ,  Guocheng Du ,  Peng Jin

发明人： Jian Chen ,  Zhen Kang ,  Guocheng Du ,  Peng Jin

IPC分类号： C07K14/00 ,  C12P19/26 ,  C12N9/26 ,  C12N9/04 ,  C12N9/12 ,  C12N9/90 ,  C12N9/10

CPC分类号： C12P19/26 ,  C12N9/0006 ,  C12N9/1096 ,  C12N9/1241 ,  C12N9/2474 ,  C12N9/90 ,  C12Y101/01022 ,  C12Y206/01016 ,  C12Y207/07009 ,  C12Y207/07023 ,  C12Y302/01035 ,  C12Y504/0201

摘要： The present invention relates to the field of biotechnology engineering. It provides a method of constructing a recombinant Bacillus subtilis that can produce specific-molecular-weight hyaluronic acids. By integranted expression of hasA from Streptococcus zooepidemicus and overexpression of genes of HA synthetic pathway, tuaD, glmU and glmS, high yield HA production was achieved in the recombinant strain. Additionally, introduction and functional expression of the leech hyaluronidase in the recombinant strain substantially increased the yield of HA to 19.38 g·L−1. Moreover, HAs with a broad range of molecular weights (103 Da to 106 MDa) were efficiently produced by controlling the expression level of hyaluronidase using RBS mutants with different translational strengths. The method of the present invention can be used to produce low molecular weight HAs at large scale in industrial applications.

公开(公告)号：US20170073719A1

公开(公告)日：2017-03-16

申请号：US15011509

申请日：2016-01-30

申请人： Jian Chen ,  Zhen Kang ,  Guocheng Du ,  Peng Jin

发明人： Jian Chen ,  Zhen Kang ,  Guocheng Du ,  Peng Jin

IPC分类号： C12P19/26 ,  C12N9/10 ,  C12N9/12 ,  C12N9/90 ,  C12N9/26 ,  C12N9/04

CPC分类号： C12P19/26 ,  C12N9/0006 ,  C12N9/1096 ,  C12N9/1241 ,  C12N9/2474 ,  C12N9/90 ,  C12Y101/01022 ,  C12Y206/01016 ,  C12Y207/07009 ,  C12Y207/07023 ,  C12Y302/01035 ,  C12Y504/0201

摘要： The present invention relates to the field of biotechnology engineering. It provides a method of constructing a recombinant Bacillus subtilis that can produce specific-molecular-weight hyaluronic acids. By integranted expression of hasA from Streptococcus zooepidemicus and overexpression of genes of HA synthetic pathway, tuaD, glmU and glmS, high yield HA production was achieved in the recombinant strain. Additionally, introduction and functional expression of the leech hyaluronidase in the recombinant strain substantially increased the yield of HA to 19.38 g·L−1. Moreover, HAs with a broad range of molecular weights (103 Da to 106 MDa) were efficiently produced by controlling the expression level of hyaluronidase using RBS mutants with different translational strengths. The method of the present invention can be used to produce low molecular weight HAs at large scale in industrial applications.

摘要翻译： 本发明涉及生物技术工程领域。 它提供了构建能产生特定分子量透明质酸的重组枯草芽孢杆菌的方法。 通过来自猪链球菌的hasA的整合表达和HA合成途径，tuaD，glmU和glmS的基因的过表达，在重组菌株中实现了高产量的HA产生。 此外，重组菌株中水蛭透明质酸酶的引入和功能表达显着提高了HA的产量至19.38g·L-1。 此外，通过使用具有不同平移强度的RBS突变体控制透明质酸酶的表达水平，有效地产生具有宽范围分子量（103Da至106Mpa）的HA。 本发明的方法可用于在工业应用中大规模生产低分子量的HAs。