公开(公告)号：US20220213542A1

公开(公告)日：2022-07-07

申请号：US17600593

申请日：2020-04-02

申请人： Jingyue Ju ,  Shiv Kumar ,  James Russo ,  Xiaoxu Li ,  Steffen Jockusch ,  Minchen Chien ,  Chuanjuan Tao

发明人： Jingyue Ju ,  Shiv Kumar ,  James Russo ,  Xiaoxu Li ,  Steffen Jockusch ,  Minchen Chien ,  Chuanjuan Tao

IPC分类号： C12Q1/6869 ,  C12Q1/6848

摘要： The invention provides nucleotide analogues and methods of use thereof for sequencing by synthesis using energy transfer dye pairs.

公开(公告)号：US20220177959A1

公开(公告)日：2022-06-09

申请号：US17436513

申请日：2020-03-05

申请人： Jingyue Ju ,  Carl W. Fuller ,  Shiv Kumar ,  Sergey Kalachikov ,  Xiaoxu Li ,  Irina Morozova ,  James Russo ,  Steffen Jockusch

发明人： Jingyue Ju ,  Carl W. Fuller ,  Shiv Kumar ,  Sergey Kalachikov ,  Xiaoxu Li ,  Irina Morozova ,  James Russo ,  Steffen Jockusch

IPC分类号： C12Q1/6869

摘要： Disclosed herein are highly accurate approaches for single molecule electronic nanopore-based SBS.

公开(公告)号：US20200063194A1

公开(公告)日：2020-02-27

申请号：US16499997

申请日：2018-04-03

申请人： Jingyue Ju ,  Timothy H. Bestor ,  James J. Russo ,  Steffen Jockusch ,  Xiaoxu Li

发明人： Jingyue Ju ,  Timothy H. Bestor ,  James J. Russo ,  Steffen Jockusch ,  Xiaoxu Li

IPC分类号： C12Q1/6827 ,  C12N9/10

摘要： The subject invention provides a method of determining whether a cytosine at a predefined position within a single strand of a double-stranded DNA of known sequence is hydroxymethylated.The invention also provides a method of determining whether a cytosine at a predefined position within a single strand of a double-stranded DNA of known sequence is unmethylated.The invention further provides a method of determining whether a cytosine at a predefined position within a single strand of a double-stranded DNA of known sequence is methylated but not hydroxymethylated.The invention also provides a method of determining whether a cytosine present at a predefined position within a single strand of a double-stranded DNA of known sequence, and within a CpG site, is unmethylated.