公开(公告)号：US20200063194A1

公开(公告)日：2020-02-27

申请号：US16499997

申请日：2018-04-03

申请人： Jingyue Ju ,  Timothy H. Bestor ,  James J. Russo ,  Steffen Jockusch ,  Xiaoxu Li

发明人： Jingyue Ju ,  Timothy H. Bestor ,  James J. Russo ,  Steffen Jockusch ,  Xiaoxu Li

IPC分类号： C12Q1/6827 ,  C12N9/10

摘要： The subject invention provides a method of determining whether a cytosine at a predefined position within a single strand of a double-stranded DNA of known sequence is hydroxymethylated.The invention also provides a method of determining whether a cytosine at a predefined position within a single strand of a double-stranded DNA of known sequence is unmethylated.The invention further provides a method of determining whether a cytosine at a predefined position within a single strand of a double-stranded DNA of known sequence is methylated but not hydroxymethylated.The invention also provides a method of determining whether a cytosine present at a predefined position within a single strand of a double-stranded DNA of known sequence, and within a CpG site, is unmethylated.

公开(公告)号：US10337049B2

公开(公告)日：2019-07-02

申请号：US14973637

申请日：2015-12-17

申请人： Timothy H. Bestor ,  Jingyue Ju ,  Xiaoxu Li ,  James J. Russo

发明人： Timothy H. Bestor ,  Jingyue Ju ,  Xiaoxu Li ,  James J. Russo

IPC分类号： C12Q1/68 ,  C12Q1/6806 ,  C07H19/16 ,  C07H19/167 ,  C12Q1/6874 ,  C12N9/10 ,  C12P19/34 ,  C12Q1/6827

摘要： The present invention provides a method of determining whether cytosine residues present at a predetermined positions within a single strand of a double stranded DNA of known sequence are methylated as well as compounds for carrying out this method.

公开(公告)号：US20160355542A1

公开(公告)日：2016-12-08

申请号：US14973637

申请日：2015-12-17

申请人： Timothy H. Bestor ,  Jingyue Ju ,  Xiaoxu Li ,  James J. Russo

发明人： Timothy H. Bestor ,  Jingyue Ju ,  Xiaoxu Li ,  James J. Russo

IPC分类号： C07H19/16 ,  C12Q1/68 ,  C12N9/10 ,  C12P19/34

CPC分类号： C12Q1/6806 ,  C07H19/16 ,  C07H19/167 ,  C12N9/1007 ,  C12P19/34 ,  C12Q1/6827 ,  C12Q1/6874 ,  C12Y201/01 ,  C12Q2535/101 ,  C12Q2535/122 ,  C12Q2563/101 ,  C12Q2565/631 ,  C12Q2563/167 ,  C12Q2563/107

摘要： The present invention provides a method of determining whether cytosine residues present at a predetermined positions within a single strand of a double stranded DNA of known sequence are methylated as well as compounds for carrying out this method.

摘要翻译： 本发明提供了确定存在于已知序列的双链DNA的单链内的预定位置处的胞嘧啶残基是否被甲基化的方法以及用于实施该方法的化合物。

公开(公告)号：US09738922B2

公开(公告)日：2017-08-22

申请号：US14578125

申请日：2014-12-19

申请人： Timothy H. Bestor ,  John R. Edwards ,  Jingyue Ju ,  Xiaoxu Li

发明人： Timothy H. Bestor ,  John R. Edwards ,  Jingyue Ju ,  Xiaoxu Li

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H19/23

CPC分类号： C12Q1/6806 ,  C07H19/23 ,  C12Q1/6827 ,  C12Q1/6874 ,  C12Q2521/125 ,  C12Q2527/125 ,  C12Q2537/164

摘要： This invention provides methods of derivatizing a double-stranded DNA comprising contacting double-stranded DNA with a CpG methyltransferase and an s-adenosylmethionine analog. This invention also provides methods of sequencing DNA to determine methylation patterns.

公开(公告)号：US20110177508A1

公开(公告)日：2011-07-21

申请号：US13055208

申请日：2009-07-22

申请人： Timothy H. Bestor ,  John R. Edwards ,  Jingyue Ju ,  Xiaoxu Li

发明人： Timothy H. Bestor ,  John R. Edwards ,  Jingyue Ju ,  Xiaoxu Li

IPC分类号： C12Q1/68 ,  C07H19/167 ,  C12N9/96 ,  C12P19/34 ,  C07H21/04 ,  C07H19/23

CPC分类号： C12Q1/6806 ,  C07H19/23 ,  C12Q1/6827 ,  C12Q1/6874 ,  C12Q2521/125 ,  C12Q2527/125 ,  C12Q2537/164

摘要： This invention provides methods of derivatizing a double-stranded DNA comprising contacting double-stranded DNA with a CpG methyltransferase and an s-adenosylmethionine analog. This invention also provides methods of sequencing DNA to determine methylation patterns. This invention also provides neobases and methods of sequencing for methylation patterns using neobases.

摘要翻译： 本发明提供衍生双链DNA的方法，包括使双链DNA与CpG甲基转移酶和s-腺苷甲硫氨酸类似物接触。 本发明还提供了测序DNA以测定甲基化模式的方法。 本发明还提供了新基因和使用新碱基测序甲基化模式的方法。

公开(公告)号：US20150148240A1

公开(公告)日：2015-05-28

申请号：US14578125

申请日：2014-12-19

申请人： Timothy H. Bestor ,  John R. Edwards ,  Jingyue Ju ,  Xiaoxu Li

发明人： Timothy H. Bestor ,  John R. Edwards ,  Jingyue Ju ,  Xiaoxu Li

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6806 ,  C07H19/23 ,  C12Q1/6827 ,  C12Q1/6874 ,  C12Q2521/125 ,  C12Q2527/125 ,  C12Q2537/164

摘要： This invention provides methods of derivatizing a double-stranded DNA comprising contacting double-stranded DNA with a CpG methyltransferase and an s-adenosylmethionine analog. This invention also provides methods of sequencing DNA to determine methylation patterns.

摘要翻译： 本发明提供衍生双链DNA的方法，包括使双链DNA与CpG甲基转移酶和s-腺苷甲硫氨酸类似物接触。 本发明还提供了测序DNA以测定甲基化模式的方法。