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    Methods and Compositions for Production of Blue Pigment Indigoidine
    1.
    发明申请
    Methods and Compositions for Production of Blue Pigment Indigoidine 有权
    蓝色颜料靛蓝的生产方法和组合

    公开(公告)号：US20140142314A1

    公开(公告)日：2014-05-22

    申请号：US14080024

    申请日：2013-11-14

    Applicant: Jixun Zhan

    Inventor： Jixun Zhan

    IPC: C12P17/16 ,  C07D213/90

    CPC classification number: C12P17/165 ,  C07D213/90 ,  C12N1/00 ,  C12N15/52

    Abstract: The present disclosure provides for an expression system for the production of blue pigment indigoidine. The system comprises a heterologous host cell, a DNA sequence encoding a Sc-IndB protein, and a DNA sequence encoding a Sc-IndC protein. The system may be configured for the co-expression of the Sc-IndB and Sc-IndC. DNA sequences encoding the Sc-IndB and Sc-IndC may be provided on at least one vector. Alternatively, the DNA sequences encoding the Sc-IndB and Sc-IndC may optionally be integrated into the genome of the heterologous host genome. The expression system may further comprise a sfp gene or a PPTase.

    Abstract translation: 本公开提供了用于生产蓝色靛红的表达系统。 该系统包含异源宿主细胞，编码Sc-IndB蛋白的DNA序列和编码Sc-IndC蛋白的DNA序列。 该系统可以被配置用于共表达Sc-IndB和Sc-IndC。 编码Sc-IndB和Sc-IndC的DNA序列可以在至少一个载体上提供。 或者，编码Sc-IndB和Sc-IndC的DNA序列可任选地整合到异源宿主基因组的基因组中。 表达系统还可以包含sfp基因或PPT酶。

    Methods and compositions for production of blue pigment indigoidine
    4.
    发明授权
    Methods and compositions for production of blue pigment indigoidine 有权
    用于生产蓝色靛蓝的方法和组合物

    公开(公告)号：US09243269B2

    公开(公告)日：2016-01-26

    申请号：US14080024

    申请日：2013-11-14

    Applicant: Jixun Zhan

    Inventor： Jixun Zhan

    IPC: C12P17/16 ,  C07D213/90 ,  C12N1/00 ,  C12N15/52

    CPC classification number: C12P17/165 ,  C07D213/90 ,  C12N1/00 ,  C12N15/52

    Abstract: The present disclosure provides for an expression system for the production of blue pigment indigoidine. The system comprises a heterologous host cell, a DNA sequence encoding a Sc-IndB protein, and a DNA sequence encoding a Sc-IndC protein. The system may be configured for the co-expression of the Sc-IndB and Sc-IndC. DNA sequences encoding the Sc-IndB and Sc-IndC may be provided on at least one vector. Alternatively, the DNA sequences encoding the Sc-IndB and Sc-IndC may optionally be integrated into the genome of the heterologous host genome. The expression system may further comprise a sfp gene or a PPTase.

    Abstract translation: 本公开提供了用于生产蓝色靛红的表达系统。 该系统包含异源宿主细胞，编码Sc-IndB蛋白的DNA序列和编码Sc-IndC蛋白的DNA序列。 该系统可以被配置用于共表达Sc-IndB和Sc-IndC。 编码Sc-IndB和Sc-IndC的DNA序列可以在至少一个载体上提供。 或者，编码Sc-IndB和Sc-IndC的DNA序列可任选地整合到异源宿主基因组的基因组中。 表达系统还可以包含sfp基因或PPT酶。

    HALOGENATION ENZYMES
    5.
    发明申请
    HALOGENATION ENZYMES 审中-公开
    卤化酶

    公开(公告)号：US20130244296A1

    公开(公告)日：2013-09-19

    申请号：US13602136

    申请日：2012-09-01

    Applicant: Jixun Zhan

    Inventor： Jixun Zhan

    IPC: C12N9/00 ,  C12P17/08 ,  C12P7/26 ,  C12P17/18

    CPC classification number: C12N9/0071 ,  C12P7/26 ,  C12P17/08 ,  C12P17/181 ,  C12P19/62 ,  C12Y114/14007

    Abstract: The present disclosure relates to an isolated or purified nucleotide sequence comprising a cDNA sequence of an rdc2 at least 60%, 70%, 90%, 95%, 98%, or 100% identical to SEQ ID NO. 1. In a second embodiment, the invention provides for a flavin-dependent halogenase comprising an amino acid sequence of an Rdc2 halogenase at least 60%, 70%, 90%, 95%, 98%, or 100% identical to SEQ ID NO. 2. In related embodiments there are provided herein methods for halogenating compounds by using an Rdc2 halogenase of the present invention.

    Abstract translation: 本公开涉及分离或纯化的核苷酸序列，其包含与SEQ ID NO：1的至少60％，70％，90％，95％，98％或100％同一性的rdc2的cDNA序列。 在第二个实施方案中，本发明提供一种黄素依赖性卤化酶，其包含与SEQ ID NO的至少60％，70％，90％，95％，98％或100％同一性的Rdc2卤代酶的氨基酸序列 。 在相关实施方案中，本文提供了使用本发明的Rdc2卤素酶使化合物卤化的方法。

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