公开(公告)号：US20170247732A1

公开(公告)日：2017-08-31

申请号：US15594860

申请日：2017-05-15

Applicant: The Board of Regents of the University of Oklahoma

Inventor： Paul L. DeAngelis ,  Nigel J. Otto

IPC: C12P19/26 ,  C08B37/00 ,  C12N9/04 ,  C12N15/52 ,  C12N9/10 ,  C12N9/12

CPC classification number: C12P19/26 ,  C08B37/0063 ,  C12N9/0006 ,  C12N9/1051 ,  C12N9/1241 ,  C12N15/52 ,  C12P19/04 ,  C12Y101/01022 ,  C12Y207/07009

Abstract: Testosteronan, a heparosan analog having the structure [-4-D-GlcUA-α1,4-D-GlcNAc-α1-]n, is produced by testosteronan synthase, a single protein that is a dual-action catalyst that utilizes UDP-GlcUA and UDP-GlcNAc to synthesize a polysaccharide having the structure [-4-D-GlcUA-α1,4-D-GlcNAc-α1-]n.

公开(公告)号：US20190194714A1

公开(公告)日：2019-06-27

申请号：US16325847

申请日：2017-08-07

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Hitoshi MUGURUMA ,  Atsunori HIRATSUKA ,  Hisanori IWASA ,  Jun TAKAGI ,  Hiroyasu KADOYA

IPC: C12Q1/00 ,  G01N27/327 ,  C01B32/158 ,  C12N9/04

CPC classification number: C12Q1/006 ,  B82Y30/00 ,  B82Y40/00 ,  C01B32/158 ,  C01B2202/02 ,  C12M1/40 ,  C12N9/0006 ,  C12Q1/32 ,  C12Y101/01022 ,  G01N27/327 ,  G01N27/3272 ,  G01N27/416

Abstract: A reagent used for a glucose sensor for electrochemical, quantitative determination of glucose, includes a flavin adenine dinucleotide glucose dehydrogenase, single-walled carbon nanotubes, and a dispersant.

公开(公告)号：US20160237465A1

公开(公告)日：2016-08-18

申请号：US15045704

申请日：2016-02-17

Applicant: FIDIA FARMACEUTICI S.P.A.

Inventor： Vincenza CORSA ,  Alessandro NEGRO ,  Susanna VACCARO ,  Luciano MESSINA

IPC: C12P19/26 ,  C12N1/20 ,  C12N9/12 ,  C12N9/92 ,  C12N9/10 ,  C12N9/04

CPC classification number: C12P19/26 ,  C08B37/0072 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/1051 ,  C12N9/1241 ,  C12N9/92 ,  C12N15/635 ,  C12N15/75 ,  C12Y101/01022 ,  C12Y204/01212 ,  C12Y207/07009 ,  C12Y503/01009

Abstract: The present invention relates to a method for the production of hyaluronic acid (HA) in Bacillus subtilis and Escherichia coli through plasmid vectors wherein the gene is under the control of strong promoter Pgrac, and a system for the selection of stable bacterial strains for the production of high levels of hyaluronic acid.

公开(公告)号：US12071636B2

公开(公告)日：2024-08-27

申请号：US17478023

申请日：2021-09-17

Applicant: The United States of America, as represented by the Secretary of Agriculture

Inventor： Robert E Briggs ,  Fred M Tatum

IPC: C12N9/16 ,  A61K39/102 ,  C12N9/04 ,  A61K39/00

CPC classification number: C12N9/0006 ,  A61K39/102 ,  C12N9/16 ,  C12Y101/01022 ,  C12Y301/03029 ,  A61K2039/522 ,  A61K2039/552

Abstract: The present invention provides novel attenuated Pasteurella multocida strains that may be used, in live or killed form, to formulate vaccines that are highly protective against P. multocida infection in bovines, other mammals, and in birds. The present invention also identifies the combination of nanP and hyaC gene mutations as key to the provision of such vaccines. When appropriately formulated, antigenic material of numerous other bovine pathogens may be combined with the live attenuated Pasteurella multocida strains, to make effective combination vaccines.

公开(公告)号：US09695453B2

公开(公告)日：2017-07-04

申请号：US14884274

申请日：2015-10-15

Applicant: FIDIA FARMACEUTICI S.P.A.

Inventor： Vincenza Corsa ,  Alessandro Negro ,  Sonia Bisicchia

IPC: C12P19/26 ,  C12P19/04 ,  C08B37/08 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/92 ,  C12N15/75

CPC classification number: C12P19/04 ,  C08B37/0072 ,  C12N9/0006 ,  C12N9/1051 ,  C12N9/1241 ,  C12N9/92 ,  C12N15/75 ,  C12P19/26 ,  C12Y101/01022 ,  C12Y204/01212 ,  C12Y207/07009 ,  C12Y503/01009

Abstract: A method of producing hyaluronic acid (HA) in Escherichia coli and Bacillus megaterium through episomal plasmid vectors wherein the gene is under the control of strong promoter T7, preferably under the control of strong promoter T7 of bacteriophage T7, and a system for the selection of stable bacterial strains producing high levels of hyaluronic acid, are provided.

公开(公告)号：US20140099673A1

公开(公告)日：2014-04-10

申请号：US13821953

申请日：2011-09-09

Applicant: Vincenza Corsa ,  Alessandro Negro ,  Sonia Bisicchia

Inventor： Vincenza Corsa ,  Alessandro Negro ,  Sonia Bisicchia

IPC: C12P19/26

CPC classification number: C12P19/04 ,  C08B37/0072 ,  C12N9/0006 ,  C12N9/1051 ,  C12N9/1241 ,  C12N9/92 ,  C12N15/75 ,  C12P19/26 ,  C12Y101/01022 ,  C12Y204/01212 ,  C12Y207/07009 ,  C12Y503/01009

Abstract: The present invention relates to a method of producing hyaluronic acid (HA) in Escherichia coli and Bacillus megaterium through episomal plasmid vectors wherein the gene is under the control of strong promoter T7, preferably under the control of strong promoter T7 of bacteriophage T7, and a system for the selection of stable bacterial strains producing high levels of hyaluronic acid.

Abstract translation: 本发明涉及通过附加型质粒载体在大肠杆菌和巨大芽孢杆菌中生产透明质酸（HA）的方法，其中该基因处于强启动子T7的控制下，优选在噬菌体T7的强启动子T7的控制下，和 用于选择产生高水平透明质酸的稳定细菌菌株的系统。

公开(公告)号：US20230142611A1

公开(公告)日：2023-05-11

申请号：US17822733

申请日：2022-08-26

Applicant: Jixun Zhan

Inventor： Jixun Zhan

IPC: C12P19/60 ,  C12N15/62 ,  C12N9/10 ,  C12N9/04 ,  C12N9/12 ,  A61K31/05 ,  C12N15/63

CPC classification number: C12P19/60 ,  C12N15/62 ,  C12N9/1051 ,  C12N9/0006 ,  C12N9/1241 ,  A61K31/05 ,  C12N15/63 ,  C12Y204/01017 ,  C12Y101/01022 ,  C12Y207/07009

Abstract: The present invention relates to an in vitro or in vivo process for producing a glucuronide comprising a glucuronic acid moiety bound to a phenolic hydroxyl group or a phenolic carboxyl group. Also provided are expression vectors, nucleic acids, polypeptides, and recombinant microbial cells useful in carrying out the process and prodrugs produced by the process.

公开(公告)号：US10017802B2

公开(公告)日：2018-07-10

申请号：US15045704

申请日：2016-02-17

Applicant: FIDIA FARMACEUTICI S.P.A.

Inventor： Vincenza Corsa ,  Alessandro Negro ,  Susanna Vaccaro ,  Luciano Messina

IPC: C12P19/26 ,  C08B37/08 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/92 ,  C12N15/75 ,  C12N1/20 ,  C12N15/63

CPC classification number: C12P19/26 ,  C08B37/0072 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/1051 ,  C12N9/1241 ,  C12N9/92 ,  C12N15/635 ,  C12N15/75 ,  C12Y101/01022 ,  C12Y204/01212 ,  C12Y207/07009 ,  C12Y503/01009

Abstract: The present invention relates to a method for the production of hyaluronic acid (HA) in Bacillus subtilis and Escherichia coli through plasmid vectors wherein the gene is under the control of strong promoter Pgrac, and a system for the selection of stable bacterial strains for the production of high levels of hyaluronic acid.

公开(公告)号：US09771607B2

公开(公告)日：2017-09-26

申请号：US15011509

申请日：2016-01-30

Applicant: Jian Chen ,  Zhen Kang ,  Guocheng Du ,  Peng Jin

Inventor： Jian Chen ,  Zhen Kang ,  Guocheng Du ,  Peng Jin

IPC: C07K14/00 ,  C12P19/26 ,  C12N9/26 ,  C12N9/04 ,  C12N9/12 ,  C12N9/90 ,  C12N9/10

CPC classification number: C12P19/26 ,  C12N9/0006 ,  C12N9/1096 ,  C12N9/1241 ,  C12N9/2474 ,  C12N9/90 ,  C12Y101/01022 ,  C12Y206/01016 ,  C12Y207/07009 ,  C12Y207/07023 ,  C12Y302/01035 ,  C12Y504/0201

Abstract: The present invention relates to the field of biotechnology engineering. It provides a method of constructing a recombinant Bacillus subtilis that can produce specific-molecular-weight hyaluronic acids. By integranted expression of hasA from Streptococcus zooepidemicus and overexpression of genes of HA synthetic pathway, tuaD, glmU and glmS, high yield HA production was achieved in the recombinant strain. Additionally, introduction and functional expression of the leech hyaluronidase in the recombinant strain substantially increased the yield of HA to 19.38 g·L−1. Moreover, HAs with a broad range of molecular weights (103 Da to 106 MDa) were efficiently produced by controlling the expression level of hyaluronidase using RBS mutants with different translational strengths. The method of the present invention can be used to produce low molecular weight HAs at large scale in industrial applications.

公开(公告)号：US20170073719A1

公开(公告)日：2017-03-16

申请号：US15011509

申请日：2016-01-30

Applicant: Jian Chen ,  Zhen Kang ,  Guocheng Du ,  Peng Jin

Inventor： Jian Chen ,  Zhen Kang ,  Guocheng Du ,  Peng Jin

IPC: C12P19/26 ,  C12N9/10 ,  C12N9/12 ,  C12N9/90 ,  C12N9/26 ,  C12N9/04

CPC classification number: C12P19/26 ,  C12N9/0006 ,  C12N9/1096 ,  C12N9/1241 ,  C12N9/2474 ,  C12N9/90 ,  C12Y101/01022 ,  C12Y206/01016 ,  C12Y207/07009 ,  C12Y207/07023 ,  C12Y302/01035 ,  C12Y504/0201

Abstract: The present invention relates to the field of biotechnology engineering. It provides a method of constructing a recombinant Bacillus subtilis that can produce specific-molecular-weight hyaluronic acids. By integranted expression of hasA from Streptococcus zooepidemicus and overexpression of genes of HA synthetic pathway, tuaD, glmU and glmS, high yield HA production was achieved in the recombinant strain. Additionally, introduction and functional expression of the leech hyaluronidase in the recombinant strain substantially increased the yield of HA to 19.38 g·L−1. Moreover, HAs with a broad range of molecular weights (103 Da to 106 MDa) were efficiently produced by controlling the expression level of hyaluronidase using RBS mutants with different translational strengths. The method of the present invention can be used to produce low molecular weight HAs at large scale in industrial applications.

Abstract translation: 本发明涉及生物技术工程领域。 它提供了构建能产生特定分子量透明质酸的重组枯草芽孢杆菌的方法。 通过来自猪链球菌的hasA的整合表达和HA合成途径，tuaD，glmU和glmS的基因的过表达，在重组菌株中实现了高产量的HA产生。 此外，重组菌株中水蛭透明质酸酶的引入和功能表达显着提高了HA的产量至19.38g·L-1。 此外，通过使用具有不同平移强度的RBS突变体控制透明质酸酶的表达水平，有效地产生具有宽范围分子量（103Da至106Mpa）的HA。 本发明的方法可用于在工业应用中大规模生产低分子量的HAs。