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    Chaperonin-target protein complex, method of producing the same, method of stabilizing target protein, method of immobilizing target protein, method of analyzing the structure of target protein, sustained-release formulation, and method of producing antibody against target protein
    3.
    发明申请
    Chaperonin-target protein complex, method of producing the same, method of stabilizing target protein, method of immobilizing target protein, method of analyzing the structure of target protein, sustained-release formulation, and method of producing antibody against target protein 审中-公开
    伴侣蛋白靶蛋白复合物，其制备方法，靶蛋白稳定化方法，固定靶蛋白的方法，分析靶蛋白结构的方法，缓释制剂及产生针对靶蛋白的抗体的方法

    公开(公告)号：US20070059794A1

    公开(公告)日：2007-03-15

    申请号：US10554747

    申请日：2004-04-28

    申请人： Akira Ideno ,  Jun-ichi Hata ,  Akiko Togi ,  Masahiro Furutani

    发明人： Akira Ideno ,  Jun-ichi Hata ,  Akiko Togi ,  Masahiro Furutani

    IPC分类号： C12P21/06 ,  C07H21/04 ,  C12N9/64

    CPC分类号： C12N15/62 ,  A61K38/1709 ,  C07K2319/20 ,  C07K2319/35 ,  C07K2319/40 ,  C07K2319/705 ,  G01N33/6803

    摘要： The present invention provides a chaperonin-target protein complex and a method of producing the same, and a method of stabilizing the target protein, a method of immobilizing the target protein, a method of analyzing the structure of the target protein, a sustained-release formulation, and a method of producing an antibody against the target protein. The chaperonin-target protein complex in the present invention includes a fusion protein having a chaperonin subunit and an affinity tag linked to the chaperonin subunit via a peptide bond and a target protein for which the affinity tag shows a specific affinity, wherein the target protein is bound to the affinity tag by means of the specific affinity, thereby forming a chaperonin ring structure consisting of a plurality of chaperonin subunits. The chaperonin-target protein in the present invention stabilizes the target protein and surely immobilize on a carrier without causing any change in its stereostructure.

    摘要翻译： 本发明提供了伴侣伴侣 - 靶蛋白复合物及其制造方法，使靶蛋白稳定化的方法，固定靶蛋白的方法，分析靶蛋白的结构的方法，持续释放 制剂和产生针对靶蛋白的抗体的方法。 本发明中的伴侣伴侣 - 靶蛋白复合物包括伴侣伴侣亚基和通过肽键与伴侣蛋白亚基连接的亲和标签的融合蛋白和亲和标签显示特异性亲和力的靶蛋白，其中靶蛋白为 通过特异性亲和力结合亲和标签，从而形成由多个伴侣蛋白亚基组成的伴侣蛋白环结构。 本发明中的伴侣伴侣靶蛋白稳定靶蛋白，并确定地固定在载体上，而不引起其立体结构的任何改变。

    Immunogen, composition for immulogical use and process for producing antibody using the same
    4.
    发明申请
    Immunogen, composition for immulogical use and process for producing antibody using the same 审中-公开
    免疫原，用于免疫应用的组合物和使用其制备抗体的方法

    公开(公告)号：US20070026501A1

    公开(公告)日：2007-02-01

    申请号：US10553694

    申请日：2004-04-16

    申请人： Yoshitaka Izumoto ,  Jun-ichi Hata ,  Akira Ideno ,  Masahiro Furutani

    发明人： Yoshitaka Izumoto ,  Jun-ichi Hata ,  Akira Ideno ,  Masahiro Furutani

    IPC分类号： C12P21/04 ,  C07K14/47

    CPC分类号： C12N9/90 ,  A61K39/00 ,  A61K2039/6031 ,  A61K2039/6043 ,  C07K14/70571 ,  C07K2319/00

    摘要： It is intended to provide an immunogen and a composition for immunological use by which a sufficient immune response is induced even by an antigen protein which has been considered unavailable to induce any effective immune responses for various reasons, and a method of producing an antibody using the same. The immunogen containing a fusion protein including the full-length or a part of a desired antigen protein and a folding factor or its subunit linked thereto via at least one peptide bond. Owing to the function of the folding factor, the antigen protein contained in the fusion protein is obtained as a soluble protein having a correct stereostructure. It is also possible to protect the antigen protein and from rapid decomposition in the blood of an animal having been inoculated therewith. The immunogen induces a higher immune response for an animal than an antigen protein alone. Examples of the folding factor include chaperonins and PPIases.

    摘要翻译： 本发明旨在提供一种用于免疫学用途的免疫原和组合物，即使由于各种原因也被认为不能诱导任何有效的免疫应答的抗原蛋白，甚至诱导足够的免疫应答，以及使用 相同。 含有包含所需抗原蛋白的全长或部分的融合蛋白的免疫原和通过至少一个肽键与其连接的折叠因子或其亚单位。 由于折叠因子的功能，获得融合蛋白中包含的抗原蛋白作为具有正确立体结构的可溶性蛋白质。 也可以保护抗原蛋白并免于已经接种的动物的血液中的快速分解。 免疫原诱导动物比单独的抗原蛋白更高的免疫应答。 折叠因子的实例包括伴侣蛋白和PPI酶。

    Method for immunizing animal, composition for immunization, method for producing antibody, method for producing hybridoma, and method for producing monoclonal antibody
    5.
    发明授权
    Method for immunizing animal, composition for immunization, method for producing antibody, method for producing hybridoma, and method for producing monoclonal antibody 有权
    免疫动物的方法，免疫组合物，抗体的制造方法，杂交瘤的制造方法以及单克隆抗体的制造方法

    公开(公告)号：US08901099B2

    公开(公告)日：2014-12-02

    申请号：US13681700

    申请日：2012-11-20

    申请人： Joe Chiba ,  Sekisui Chemical Co., Ltd.

    发明人： Joe Chiba ,  Jun-ichi Hata ,  Naoki Nishiguchi ,  Masahiro Furutani

    IPC分类号： A61K48/00 ,  C12N5/04 ,  C12N15/06 ,  A61K39/00 ,  A61K39/385 ,  C07K16/28

    CPC分类号： A61K39/00 ,  A61K39/0005 ,  A61K39/385 ,  A61K2039/53 ,  A61K2039/6068 ,  C07K16/2869 ,  C07K2319/35

    摘要： It is an object of the present invention to a method whereby a humoral immune response is induced more efficiently in producing an antibody against an antigen protein by gene immunization. A fusion gene composed of a gene encoding the full-length of a part of the antigen protein or a gene encoding a chaperonin subunit or a chaperonin subunit linkage linked thereto is administered to express the fusion gene in the animal, thereby inducing a humoral immune response to an antigen protein by administering. An example of the chaperonin includes Escherichia coli GroEL. There is also provided with a composition for immunization, a method for producing an antibody, a method for producing a hybridoma, and a method for producing a monoclonal antibody.

    摘要翻译： 本发明的目的是提供一种通过基因免疫在产生针对抗原蛋白的抗体中更有效地诱导体液免疫应答的方法。 施用由编码全长一部分抗原蛋白的基因或编码伴侣伴侣亚基的基因或与其相连的伴侣伴侣亚基的基因组成的融合基因，以表达动物中的融合基因，由此诱导体液免疫应答 通过给予抗原蛋白。 伴侣蛋白的一个实例包括大肠杆菌GroEL。 还提供了用于免疫的组合物，抗体的制备方法，杂交瘤的制备方法和单克隆抗体的制备方法。