公开(公告)号：US20070059794A1

公开(公告)日：2007-03-15

申请号：US10554747

申请日：2004-04-28

申请人： Akira Ideno ,  Jun-ichi Hata ,  Akiko Togi ,  Masahiro Furutani

发明人： Akira Ideno ,  Jun-ichi Hata ,  Akiko Togi ,  Masahiro Furutani

IPC分类号： C12P21/06 ,  C07H21/04 ,  C12N9/64

CPC分类号： C12N15/62 ,  A61K38/1709 ,  C07K2319/20 ,  C07K2319/35 ,  C07K2319/40 ,  C07K2319/705 ,  G01N33/6803

摘要： The present invention provides a chaperonin-target protein complex and a method of producing the same, and a method of stabilizing the target protein, a method of immobilizing the target protein, a method of analyzing the structure of the target protein, a sustained-release formulation, and a method of producing an antibody against the target protein. The chaperonin-target protein complex in the present invention includes a fusion protein having a chaperonin subunit and an affinity tag linked to the chaperonin subunit via a peptide bond and a target protein for which the affinity tag shows a specific affinity, wherein the target protein is bound to the affinity tag by means of the specific affinity, thereby forming a chaperonin ring structure consisting of a plurality of chaperonin subunits. The chaperonin-target protein in the present invention stabilizes the target protein and surely immobilize on a carrier without causing any change in its stereostructure.

摘要翻译： 本发明提供了伴侣伴侣 - 靶蛋白复合物及其制造方法，使靶蛋白稳定化的方法，固定靶蛋白的方法，分析靶蛋白的结构的方法，持续释放 制剂和产生针对靶蛋白的抗体的方法。 本发明中的伴侣伴侣 - 靶蛋白复合物包括伴侣伴侣亚基和通过肽键与伴侣蛋白亚基连接的亲和标签的融合蛋白和亲和标签显示特异性亲和力的靶蛋白，其中靶蛋白为 通过特异性亲和力结合亲和标签，从而形成由多个伴侣蛋白亚基组成的伴侣蛋白环结构。 本发明中的伴侣伴侣靶蛋白稳定靶蛋白，并确定地固定在载体上，而不引起其立体结构的任何改变。

公开(公告)号：US20070026501A1

公开(公告)日：2007-02-01

申请号：US10553694

申请日：2004-04-16

申请人： Yoshitaka Izumoto ,  Jun-ichi Hata ,  Akira Ideno ,  Masahiro Furutani

发明人： Yoshitaka Izumoto ,  Jun-ichi Hata ,  Akira Ideno ,  Masahiro Furutani

IPC分类号： C12P21/04 ,  C07K14/47

CPC分类号： C12N9/90 ,  A61K39/00 ,  A61K2039/6031 ,  A61K2039/6043 ,  C07K14/70571 ,  C07K2319/00

摘要： It is intended to provide an immunogen and a composition for immunological use by which a sufficient immune response is induced even by an antigen protein which has been considered unavailable to induce any effective immune responses for various reasons, and a method of producing an antibody using the same. The immunogen containing a fusion protein including the full-length or a part of a desired antigen protein and a folding factor or its subunit linked thereto via at least one peptide bond. Owing to the function of the folding factor, the antigen protein contained in the fusion protein is obtained as a soluble protein having a correct stereostructure. It is also possible to protect the antigen protein and from rapid decomposition in the blood of an animal having been inoculated therewith. The immunogen induces a higher immune response for an animal than an antigen protein alone. Examples of the folding factor include chaperonins and PPIases.

摘要翻译： 本发明旨在提供一种用于免疫学用途的免疫原和组合物，即使由于各种原因也被认为不能诱导任何有效的免疫应答的抗原蛋白，甚至诱导足够的免疫应答，以及使用 相同。 含有包含所需抗原蛋白的全长或部分的融合蛋白的免疫原和通过至少一个肽键与其连接的折叠因子或其亚单位。 由于折叠因子的功能，获得融合蛋白中包含的抗原蛋白作为具有正确立体结构的可溶性蛋白质。 也可以保护抗原蛋白并免于已经接种的动物的血液中的快速分解。 免疫原诱导动物比单独的抗原蛋白更高的免疫应答。 折叠因子的实例包括伴侣蛋白和PPI酶。

公开(公告)号：US20050130259A1

公开(公告)日：2005-06-16

申请号：US10511098

申请日：2003-06-25

申请人： Akira Ideno ,  Tadashi Maruyama ,  Masahiro Furutani

发明人： Akira Ideno ,  Tadashi Maruyama ,  Masahiro Furutani

IPC分类号： C07K19/00 ,  C12N1/21 ,  C12N15/62 ,  C12N15/70 ,  C12P21/02 ,  C07H21/04 ,  C12N9/16 ,  C12P21/04 ,  C12P21/06

CPC分类号： C12N15/70 ,  C07K2319/35 ,  C07K2319/50 ,  C12N15/62 ,  C12P21/02

摘要： It is an object of the present invention to provide an expression vector, a host, a fused protein, a protein, a process for producing a fused protein, and a process for producing a protein, which can prevent formation of an unactive abnormal protein at production of a recombinant protein, and can produce a desired protein as a natural type, that is, a soluble type at a large amount and effectively. That is, the present invention is an expression vector, which comprises: (a) a first coding region encoding a polypeptide having molecular chaperone activity, and (b) a region having at least one restriction enzyme site in which a second coding region encoding a protein can be inserted. In the expression vector of the present invention, the first coding region is operatively linked to a promoter, and the restriction enzyme sites are in the same reading frame as the first coding region, and are downstream of the first coding region, or the restriction enzyme sites are disposed so that the inserted second coding region is operatively linked to a promoter, and the first coding region is in the same reading frame as the second coding region, and is downstream of the second coding region.

摘要翻译： 本发明的目的是提供表达载体，宿主，融合蛋白，蛋白质，产生融合蛋白的方法以及制备蛋白质的方法，其可以防止非活性异常蛋白质的形成 生产重组蛋白质，并且可以以大量和有效地产生期望的天然蛋白质，即可溶型。 也就是说，本发明是一种表达载体，其包括：（a）编码具有分子伴侣活性的多肽的第一编码区，和（b）具有至少一个限制酶位点的区域，其中编码 蛋白质可以插入。 在本发明的表达载体中，第一编码区与启动子可操作地连接，限制酶位点与第一编码区位于相同的阅读框中，并且位于第一编码区的下游，或限制酶 布置位置使得插入的第二编码区域可操作地连接到启动器，并且第一编码区域与第二编码区域处于相同的读取框中，并且在第二编码区域的下游。

公开(公告)号：US5103477A

公开(公告)日：1992-04-07

申请号：US569034

申请日：1990-08-17

申请人： Kimihito Harada ,  Akira Ideno

发明人： Kimihito Harada ,  Akira Ideno

IPC分类号： G06F21/10 ,  H04N7/167 ,  H04N7/171

CPC分类号： H04N7/1713 ,  H04N7/1675

摘要： An extension control signal for a vertical blanking interval (VBI) rises at a timing delayed from the last key signal before the VBI by 64 .mu.sec plus the time difference between the key signal and horizontal synchronizing signal section, and falls at a timing delayed from the first key signal after lack of the key signal detection by an extension period of the horizontal synchronizing signal section plus the above time difference.

摘要翻译： 用于垂直消隐间隔（VBI）的扩展控制信号在VBI之前的最后一个键信号延迟64μsec加上键信号和水平同步信号部分之间的时间差的定时上升，并且在从 在水平同步信号部分的延长时间段加上上述时间差之后缺少关键信号检测的第一个键信号。

公开(公告)号：US4953208A

公开(公告)日：1990-08-28

申请号：US448442

申请日：1989-12-11

申请人： Akira Ideno

发明人： Akira Ideno

IPC分类号： G06F21/10 ,  H04N7/167 ,  H04N7/171

CPC分类号： H04N7/1675 ,  H04N7/1713

摘要： A method of scrambling a television signal is provided in which, during a horizontal blanking interval and during a vertical blanking interval, a video signal is compressed and a key signal containing information concerning the compression is superimposed on an audio signal in the horizontal blanking period. The method is characterized by superimposing a pseudo-key signal on an audio signal in the vertical blanking interval, making the key signal contain information concerning the position of a vertical blanking interval, and fixing a video signal in a predetermined number of horizontal scanning periods before a vertical synchronizing signal portion in the vertical blanking interval at a constant level while eliminating color bursts in a predetermined number of horizontal scanning periods before and after the vertical synchronizing signal portion in the vertical blanking interval. In this way, unauthorized users are prohibited from receiving the signal, and unauthorized access is made difficult.

摘要翻译： 提供一种对电视信号进行加扰的方法，其中在水平消隐间隔期间和在垂直消隐间隔期间，视频信号被压缩，并且包含有关压缩的信息的键信号被叠加在水平消隐期间的音频信号上。 该方法的特征在于在垂直消隐间隔中将音频信号叠加在一个音频信号上，使得该键信号包含关于垂直消隐间隔的位置的信息，并将视频信号固定在预定数量的水平扫描周期之前 垂直消隐间隔中的垂直同步信号部分处于恒定电平，同时消除垂直消隐间隔中的垂直同步信号部分之前和之后预定数量的水平扫描周期中的色同步信号。 以这种方式，未经授权的用户被禁止接收信号，并且非法访问变得困难。