公开(公告)号：US08110399B2

公开(公告)日：2012-02-07

申请号：US11438790

申请日：2006-05-22

申请人： Joel F. Habener ,  Henryk Zulewski ,  Melissa K. Thomas ,  Elizabeth J. Abraham ,  Mario Vallejo ,  Colin A. Leech ,  Anna Louise Nolan ,  Andreas Lechner

发明人： Joel F. Habener ,  Henryk Zulewski ,  Melissa K. Thomas ,  Elizabeth J. Abraham ,  Mario Vallejo ,  Colin A. Leech ,  Anna Louise Nolan ,  Andreas Lechner

IPC分类号： C12N5/071 ,  A01N63/00 ,  A01N65/00

CPC分类号： A01K67/0271 ,  A61K35/12 ,  A61K2035/122 ,  C12N5/0678 ,  C12N2500/34 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/335 ,  C12N2510/02

摘要： Methods and compositions are described for the treatment of type I insulin-dependent diabetes mellitus and other conditions using newly identified stem cells that are capable of differentiation into a variety of pancreatic islet cells, including insulin-producing beta cells, as well as hepatocytes. Nestin and ABCG2 have been identified as molecular markers for pancreatic stem cells, while cytokeratin-19 serves as a marker for a distinct class of islet ductal cells. Methods are described whereby nestin and/or ABCG2-positive stem cells can be isolated from pancreatic islets and cultured to obtain further stem cells or pseudo-islet like structures. Methods for ex vivo differentiation of the pancreatic stem cells are disclosed. Methods are described whereby pancreatic stem cells can be isolated, expanded, and transplanted into a patient in need thereof, either allogeneically, isogeneically or xenogenically, to provide replacement for lost or damaged insulin-secreting cells or other cells.

摘要翻译： 描述了用于治疗I型胰岛素依赖性糖尿病和其它条件的方法和组合物，其使用能够分化成多种胰岛细胞（包括产生胰岛素的β细胞）以及肝细胞的新鉴定的干细胞。 Nestin和ABCG2已被鉴定为胰干细胞的分子标记，而细胞角蛋白-19可作为不同类型的胰岛导管细胞的标记物。 描述了方法，其中可以从胰岛中分离巢蛋白和/或ABCG2-阳性干细胞并进行培养以获得进一步的干细胞或假胰岛样结构。 公开了胰腺干细胞离体分化方法。 描述了方法，其中胰腺干细胞可以被分离，扩增和移植到有需要的患者中，同种异体地，异质性或异质性的，以提供丢失或损伤的胰岛素分泌细胞或其他细胞的替代。