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    METHOD FOR AFFINITY SCORING OF PEPTIDE/PROTEIN COMPLEXES
    1.
    发明申请
    METHOD FOR AFFINITY SCORING OF PEPTIDE/PROTEIN COMPLEXES 失效
    肽/蛋白复合物的亲和性分选方法

    公开(公告)号：US20120278054A1

    公开(公告)日：2012-11-01

    申请号：US13270931

    申请日：2011-10-11

    申请人： Johan Desmet ,  Geert Meersseman ,  Nathalie Boutonnet ,  Jurgen Pletinckx ,  Krista De Clercq ,  Ignace Lasters

    发明人： Johan Desmet ,  Geert Meersseman ,  Nathalie Boutonnet ,  Jurgen Pletinckx ,  Krista De Clercq ,  Ignace Lasters

    IPC分类号： G06F19/12

    CPC分类号： G06F19/16 ,  G06F19/18

    摘要： The present invention is related to a quantitative structure-based affinity scoring method for peptide/protein complexes. More specifically, the present invention comprises a method that operates on the basis of a highly specific force field function (e.g. CHARMM) that is applied to all-atom structural representations of peptide/receptor complexes. Peptide side-chain contributions to total affinity are scored after detailed rotameric sampling followed by controlled energy refinement. The method of the invention further comprises a de novo approach to estimate dehydration energies from the simulation of individual amino acids in a solvent box filled with explicit water molecules and applying the same force field function as used to evaluate peptide/receptor complex interactions.

    摘要翻译： 本发明涉及肽/蛋白复合物的基于定量结构的亲和评分方法。 更具体地说，本发明包括一种基于应用于肽/受体复合物的全原子结构表示的高度特异性的力场函数（例如CHARMM）来操作的方法。 在详细的旋转异构体取样之后进行受控能量细化，对总亲和力的肽侧链贡献进行评分。 本发明的方法还包括从填充有显性水分子的溶剂盒中的各个氨基酸的模拟估算脱水能量并应用用于评估肽/受体复合物相互作用的相同的力场函数的从头方法。

    Method for affinity scoring of peptide/protein complexes
    2.
    发明授权
    Method for affinity scoring of peptide/protein complexes 失效
    肽/蛋白复合物的亲和力评分方法

    公开(公告)号：US08538706B2

    公开(公告)日：2013-09-17

    申请号：US13270931

    申请日：2011-10-11

    申请人： Johan Desmet ,  Geert Meersseman ,  Nathalie Boutonnet ,  Jurgen Pletinckx ,  Krista De Clercq ,  Ignace Lasters

    发明人： Johan Desmet ,  Geert Meersseman ,  Nathalie Boutonnet ,  Jurgen Pletinckx ,  Krista De Clercq ,  Ignace Lasters

    IPC分类号： G06F19/16 ,  G06F19/18

    CPC分类号： G06F19/16 ,  G06F19/18

    摘要： The present invention is related to a quantitative structure-based affinity scoring method for peptide/protein complexes. More specifically, the present invention comprises a method that operates on the basis of a highly specific force field function (e.g. CHARMM) that is applied to all-atom structural representations of peptide/receptor complexes. Peptide side-chain contributions to total affinity are scored after detailed rotameric sampling followed by controlled energy refinement. The method of the invention further comprises a de novo approach to estimate dehydration energies from the simulation of individual amino acids in a solvent box filled with explicit water molecules and applying the same force field function as used to evaluate peptide/receptor complex interactions.

    摘要翻译： 本发明涉及肽/蛋白复合物的基于定量结构的亲和评分方法。 更具体地说，本发明包括一种基于应用于肽/受体复合物的全原子结构表示的高度特异性的力场函数（例如CHARMM）来操作的方法。 在详细的旋转异构体取样之后进行受控能量细化，对总亲和力的肽侧链贡献进行评分。 本发明的方法还包括从填充有显性水分子的溶剂盒中的各个氨基酸的模拟估算脱水能量并应用用于评估肽/受体复合物相互作用的相同的力场函数的从头方法。

    Method for Affinity Scoring of Peptide/Protein Complexes
    3.
    发明申请
    Method for Affinity Scoring of Peptide/Protein Complexes 审中-公开
    肽/蛋白复合物的亲和力评分方法

    公开(公告)号：US20080312840A1

    公开(公告)日：2008-12-18

    申请号：US11568108

    申请日：2006-04-21

    申请人： Johan Desmet ,  Geert Meersseman ,  Nathalie Boutonnet ,  Jurgen Pletinckx ,  Krista De Clercq ,  Ignace Lasters

    发明人： Johan Desmet ,  Geert Meersseman ,  Nathalie Boutonnet ,  Jurgen Pletinckx ,  Krista De Clercq ,  Ignace Lasters

    IPC分类号： G01N33/68

    CPC分类号： G16B15/00 ,  G16B20/00

    摘要： The present invention is related to a quantitative structure-based affinity scoring method for peptide/protein complexes. More specifically, the present invention comprises a method that operates on the basis of a highly specific force field function (e.g. CHARMM) that is applied to all-atom structural representations of peptide/receptor complexes. Peptide side-chain contributions to total affinity are scored after detailed rotameric sampling followed by controlled energy refinement. The method of the invention further comprises a de novo approach to estimate dehydration energies from the simulation of individual amino acids in a solvent box filled with explicit water molecules and applying the same force field function as used to evaluate peptide/receptor complex interactions.

    摘要翻译： 本发明涉及肽/蛋白复合物的基于定量结构的亲和评分方法。 更具体地说，本发明包括一种基于应用于肽/受体复合物的全原子结构表示的高度特异性的力场函数（例如CHARMM）来操作的方法。 在详细的旋转异构体取样之后进行受控能量细化，对总亲和力的肽侧链贡献进行评分。 本发明的方法还包括从填充有显性水分子的溶剂盒中的各个氨基酸的模拟估算脱水能量并应用用于评估肽/受体复合物相互作用的相同的力场函数的从头方法。

    ALPHABODIES SPECIFICALLY BINDING TO CYTOKINES OR GROWTH FACTORS AND/OR CYTOKINE OR GROWTH FACTOR RECEPTORS
    4.
    发明申请
    ALPHABODIES SPECIFICALLY BINDING TO CYTOKINES OR GROWTH FACTORS AND/OR CYTOKINE OR GROWTH FACTOR RECEPTORS 审中-公开
    ALPHABODIES特异性结合细胞因子或生长因子和/或细胞因子或生长因子受体

    公开(公告)号：US20140057830A1

    公开(公告)日：2014-02-27

    申请号：US13994107

    申请日：2012-01-06

    申请人： Ignace Lasters ,  Johan Desmet ,  Maria Henderikx ,  Anita Wehnert ,  Geert Meersseman

    发明人： Ignace Lasters ,  Johan Desmet ,  Maria Henderikx ,  Anita Wehnert ,  Geert Meersseman

    IPC分类号： C07K14/00

    CPC分类号： C07K14/001 ,  C07K16/244 ,  C07K16/2866 ,  C07K2317/33 ,  C07K2318/20

    摘要： Alphabodies that specifically bind to cytokines or growth factor and/or their receptors, as well as polypeptides that comprise or essentially consist of such Alphabodies. Further nucleic acids encoding such Alphabodies; methods for preparing such Alphabodies and polypeptides; host cells expressing or capable of expressing such Alphabodies and polypeptides; compositions, and in particular pharmaceutical compositions, that comprise such Alphabodies, polypeptides, nucleic acids and/or host cells; and uses of such Alphabodies or polypeptides, nucleic acids, host cells and/or compositions, in particular for prophylactic, therapeutic or diagnostic purposes.

    摘要翻译： 与细胞因子或生长因子和/或其受体特异性结合的抗体，以及包含或基本上由此类Alphabody组成的多肽。 编码这样的阿片受体的另外的核酸; 制备此类抗体和多肽的方法; 表达或能够表达此类α受体和多肽的宿主细胞; 组合物，特别是药物组合物，其包含这样的阿片受体，多肽，核酸和/或宿主细胞; 以及这些阿拉伯抗体或多肽，核酸，宿主细胞和/或组合物的用途，特别是用于预防，治疗或诊断目的。

    ALPHABODIES SPECIFICALLY BINDING TO VIRAL PROTEINS AND METHODS FOR PRODUCING THE SAME
    5.
    发明申请
    ALPHABODIES SPECIFICALLY BINDING TO VIRAL PROTEINS AND METHODS FOR PRODUCING THE SAME 审中-公开
    特异性绑定到病毒蛋白质的方法及其生产方法

    公开(公告)号：US20140294828A1

    公开(公告)日：2014-10-02

    申请号：US13994106

    申请日：2011-01-06

    申请人： Johan Desmet ,  Ignace Lasters ,  Geert Meersseman ,  Sabrina Deroo

    发明人： Johan Desmet ,  Ignace Lasters ,  Geert Meersseman ,  Sabrina Deroo

    IPC分类号： C07K16/08

    CPC分类号： C07K16/08 ,  A61K39/00 ,  C07K16/1063 ,  C07K2317/92 ,  C07K2318/20 ,  G01N33/56988 ,  Y02A50/53 ,  Y02A50/60

    摘要： The invention provides methods for the production of single-chain Alphabody polypeptides having detectable binding affinity for, or detectable in vitro activity on, a viral protein of interest, which comprising the step of producing a single-chain Alphabody library comprising at least 100 different-sequence single-chain Alphabody polypeptides, wherein said Alphabody polypeptides differ from each other in at least one of a defined set of 5 to 20 variegated amino acid residue positions, and wherein said variegated amino acid residue positions are located at specific positions in one or more of the alpha-helices of the Alphabody or the linker fragment connecting two consecutive alpha-helices of the Alphabody polypeptides. The invention further provides Alphabodies obtainable by the methods of the invention and uses thereof.

    摘要翻译： 本发明提供了生产具有对感兴趣的病毒蛋白质具有可检测的结合亲和力或可检测的体外活性的单链阿拉伯糖苷多肽的方法，其包括产生包含至少100个不同序列的单链Alphabody文库的步骤， 序列单链Alphabody多肽，其中所述Alphabody多肽在限定的5至20个杂化氨基酸残基位置中的至少一个中彼此不同，并且其中所述杂色氨基酸残基位置位于一个或多个 的Alphabody的α-螺旋或连接Alphabody多肽的两个连续的α-螺旋的连接物片段。 本发明还提供了可通过本发明的方法获得的阿尔培布本及其用途。

    Method, computing routine, device for predicting properties of MHC/peptide complexes, and data and peptides produced therefrom
    8.
    发明授权
    Method, computing routine, device for predicting properties of MHC/peptide complexes, and data and peptides produced therefrom 有权
    方法，计算程序，用于预测MHC /肽复合物的性质的装置，以及由其制备的数据和肽

    公开(公告)号：US07702465B2

    公开(公告)日：2010-04-20

    申请号：US10516628

    申请日：2003-06-10

    申请人： Ignace Lasters ,  Johan Desmet

    发明人： Ignace Lasters ,  Johan Desmet

    IPC分类号： G06F19/00 ,  C07K14/74 ,  C07K14/435

    CPC分类号： G06F19/16 ,  G06F19/18

    摘要： The present invention relates to a method for structure-based prediction of properties of peptides and peptide analogs in complex with major histocompatibility (MHC) class I and class II molecules. The properties mainly relate to the three-dimensional structure of an MHC/peptide complex and the binding affinity of a peptide for an MHC receptor. The invention further relates to a computer program and a device therefor. The invention further relates to data produced by a method of the invention. The invention further relates to peptides and peptide analogs predicted to bind to target-MHC molecules. The present invention thus relates to the field of immunology, with possible applications in manufacture of vaccinates, de-immunization of proteins, and manufacture of therapeutic agents, especially immunotherapeutic agents.

    摘要翻译： 本发明涉及与主要组织相容性（MHC）I类和II类分子复合的肽和肽类似物的基于结构的预测性质的方法。 性质主要涉及MHC /肽复合物的三维结构和肽对MHC受体的结合亲和力。 本发明还涉及一种计算机程序及其装置。 本发明还涉及通过本发明的方法产生的数据。 本发明还涉及预期与靶MHC分子结合的肽和肽类似物。 因此，本发明涉及免疫学领域，可能应用于疫苗接种的制造，蛋白质的去免疫，以及治疗剂，特别是免疫治疗剂的制造。

    Non-natural proteinaceous scaffold made of three non-covalently associated peptides
    9.
    发明授权
    Non-natural proteinaceous scaffold made of three non-covalently associated peptides 有权
    非天然蛋白质支架由三个非共价相关肽组成

    公开(公告)号：US09217011B2

    公开(公告)日：2015-12-22

    申请号：US12676783

    申请日：2008-09-08

    申请人： Johan Desmet ,  Ignace Lasters

    发明人： Johan Desmet ,  Ignace Lasters

    IPC分类号： C07K14/00 ,  C07K1/00 ,  C07K7/08

    CPC分类号： C07K1/00 ,  C07K7/08 ,  C07K14/001 ,  C07K2318/20

    摘要： The present invention is related to a non-natural, thermodynamically stable, proteinaceous scaffold consisting of three non-covalently associated peptides, wherein each peptide sequence comprises less than fifty amino acid residues and wherein at least 50% of the said residues are substitutable amino acids into at least ten different amino acid residue types. The present invention is further related to a non-natural, triple-stranded, parallel alpha-helical coiled coil scaffold wherein each of the three constituting peptide sequences comprise between 2 and 7 consecutive heptad repeats of the formula cxxcxxx (SEQ ID NO: 10), wherein at least 70% of the core c-residues are isoleucines, wherein all non-core x-residues are alanines, and wherein the constituting peptide sequences remain associated under physical conditions that are significantly different from physiological conditions.

    摘要翻译： 本发明涉及由三种非共价相关肽组成的非天然的热力学稳定的蛋白质支架，其中每个肽序列包含少于50个氨基酸的残基，并且其中至少50％的所述残基是可取代的氨基酸 至少十种不同的氨基酸残基类型。 本发明进一步涉及非天然的三链平行α-螺旋卷曲线圈支架，其中三个构成肽序列中的每一个包含2至7个连续的式cxxcxxx（SEQ ID NO：10）的七重复重复序列， 其中至少70％的核心c残基是异亮氨酸，其中所有非核心x残基都是丙氨酸，并且其中构成的肽序列在与生理条件显着不同的物理条件下保持相关。