公开(公告)号：US10124128B2

公开(公告)日：2018-11-13

申请号：US10298177

申请日：2002-11-14

申请人： John D. Burr ,  Jeff R. Wood ,  Adrian E. Smith ,  John A. Howard ,  Fabrizio Niccolai

发明人： John D. Burr ,  Jeff R. Wood ,  Adrian E. Smith ,  John A. Howard ,  Fabrizio Niccolai

IPC分类号： A61M15/08 ,  A61M15/00

摘要： An aerosolization apparatus comprises a body having an inlet, an endpiece having an outlet, the endpiece being connectable to the body to define a chamber, wherein the chamber is sized to receive a capsule containing a pharmaceutical formulation in a manner which allows the capsule to move within the chamber. The apparatus further includes a connection mechanism to provide selective connection of the endpiece to the body, wherein a rotational force between the endpiece and the body is needed to connect or disconnect the endpiece from the body, the rotational force being applied about an axis passing through the chamber. When a user inhales, air enters into the chamber through the inlet so that the pharmaceutical formulation is aerosolized within the chamber and the aerosolized pharmaceutical formulation is delivered to the user through the outlet. The connection mechanism prevents inadvertent disconnection of the endpiece from the body.

公开(公告)号：US07071384B2

公开(公告)日：2006-07-04

申请号：US10164775

申请日：2002-06-06

申请人： John A. Howard ,  Elizabeth E. Hood ,  Michele Bailey Piatchek

发明人： John A. Howard ,  Elizabeth E. Hood ,  Michele Bailey Piatchek

IPC分类号： C12N15/82

CPC分类号： C07K14/415 ,  C12N15/8234 ,  C12N15/8257

摘要： A method for commercial production and use of heterologous laccase from plant seed in commercial processes is shown. The method comprises degerminating the germ portion of the seed and using the germ as the source of laccase in a commercial process. Increased concentration of laccase on a dry weight basis is achieved. Increased cost recovery is obtained in the process.

摘要翻译： 显示了在商业过程中从植物种子商业生产和使用异源漆酶的方法。 该方法包括在商业过程中使种子的胚芽部分脱粒并使用胚芽作为漆酶的来源。 实现了漆酶浓度在干重上的增加。 在此过程中获得了更高的成本回收。

公开(公告)号：US6087558A

公开(公告)日：2000-07-11

申请号：US120582

申请日：1998-07-22

申请人： John A. Howard ,  Elizabeth Hood

发明人： John A. Howard ,  Elizabeth Hood

IPC分类号： C12N9/76 ,  C12N15/55 ,  C12N15/82 ,  A01H5/10 ,  A01H5/00 ,  C12N5/04 ,  C12N15/12 ,  C12N15/57

CPC分类号： C12N9/6427 ,  C12N15/8257

摘要： Production of proteases in plants is set forth, whereby heterologous DNA encoding the protease is introduced into the plant and expression of the protein achieved. By such methods, expression is achieved in plants wherein the plant cell is not damaged, the protein can be recovered without contamination by other proteases, and can be expressed at levels such that commercial production of the enzyme is obtained. Expression levels can be at 0.1% of total soluble protein of the plant, or higher.

摘要翻译： 阐述植物中蛋白酶的产生，由此将编码蛋白酶的异源DNA引入植物中并实现蛋白质的表达。 通过这样的方法，在植物细胞未受损害的植物中实现表达，可以在不受其它蛋白酶污染的情况下回收蛋白质，并且可以以获得酶的商业生产的水平来表达。 表达水平可以在植物总可溶性蛋白质的0.1％以上。

公开(公告)号：USD288139S

公开(公告)日：1987-02-03

申请号：US587747

申请日：1984-03-09

申请人： Joost van Berne ,  Gerardus J. van der Vlies ,  John A. Howard

设计人： Joost van Berne ,  Gerardus J. van der Vlies ,  John A. Howard

公开(公告)号：US06632930B2

公开(公告)日：2003-10-14

申请号：US09881165

申请日：2001-06-14

申请人： Elizabeth Hood ,  John A. Howard ,  Michele Bailey ,  Franciscus J. C. van Gastel ,  Michael Ward ,  Huaming Wang ,  Susan Woodard

发明人： Elizabeth Hood ,  John A. Howard ,  Michele Bailey ,  Franciscus J. C. van Gastel ,  Michael Ward ,  Huaming Wang ,  Susan Woodard

IPC分类号： A23J100

CPC分类号： C12Y110/03002 ,  C12N9/0061 ,  C12N9/16 ,  C12N9/96 ,  C12N15/8257

摘要： A method of increasing recovery of active enzyme produced in a plant is provided where the enzyme requires a transitional metal cofactor for activation. The metal cofactor is supplied to the enzyme during plant development, during extraction, or after extraction. Recovery of active enzyme is also provided by incubating the extracted enzyme at a non-enzyme degrading temperature. Addition of a negative ion salt further improves active enzyme recovery. Optimum salt concentrations for recovery of laccase from plants using copper solutions is provided.

摘要翻译： 提供了增加植物中产生的活性酶的回收率的方法，其中酶需要用于活化的过渡金属辅因子。 在植物发育，提取期间或提取后，将金属辅因子提供给酶。 通过在非酶降解温度下温育提取的酶也可提供活性酶的回收。 加入负离子盐进一步提高了活性酶的回收率。 提供了使用铜溶液从植物中回收漆酶的最佳盐浓度

公开(公告)号：US06504085B1

公开(公告)日：2003-01-07

申请号：US09403686

申请日：1999-09-07

申请人： John A. Howard

发明人： John A. Howard

IPC分类号： A01H500

CPC分类号： C07K14/415 ,  C12N15/8234 ,  C12N15/8257

摘要： A method for extraction of heterologous protein from plant seed comprises extracting the germ portion of the seed and extracting and purifying the protein from the germ. Enhanced expression in the germ is provided, and allows for improved efficiency in production, and cost savings. Directing expression to the germ portion further increases expression levels of the protein. The ubiquitin promoter preferentially directs expression to the germ portion of plant seed.

摘要翻译： 从植物种子提取异源蛋白质的方法包括提取种子的胚芽部分并从细菌中提取和纯化蛋白质。 提供了细菌的增强表达，并且可以提高生产效率和节约成本。 将表达引导到胚芽部分进一步增加蛋白质的表达水平。 泛素启动子优先将表达指导到植物种子的胚芽部分。

公开(公告)号：US5824870A

公开(公告)日：1998-10-20

申请号：US554161

申请日：1995-11-06

申请人： Chris Baszczynski ,  Thomas Czapla ,  Elizabeth Hood ,  Terry EuClaire Meyer ,  David Peterson ,  A. Gururaj Rao ,  James C. Register, III ,  Derrick Witcher ,  John A. Howard

发明人： Chris Baszczynski ,  Thomas Czapla ,  Elizabeth Hood ,  Terry EuClaire Meyer ,  David Peterson ,  A. Gururaj Rao ,  James C. Register, III ,  Derrick Witcher ,  John A. Howard

IPC分类号： C07K14/81 ,  C12N15/15 ,  C12N15/29 ,  C12N15/82 ,  C12N15/12 ,  A01H5/00

CPC分类号： C07K14/8117 ,  C12N15/8257 ,  C12N15/8286

摘要： A method for commercial production of aprotinin entails heterologous expression of the protein in plants, preferably at a level such that aprotinin accounts for at least 0.1% of the total extracted protein. An aprotinin-expressing plant also has potential of increased insecticidal resistance by virtue of producing the protein. A genetic map of the integration locus allows identification of plants derived from the transgenic plant. This approach also reveals genetic loci on a plant chromosome that support high levels of gene expression and can be used as site of integration for expression of other genes of interest.

摘要翻译： 抑肽酶的商业生产方法需要在植物中异源表达蛋白质，优选地使得抑肽酶占总提取蛋白质的至少0.1％。 表达抑肽酶的植物还具有通过产生蛋白质而具有增加的杀虫抗性的潜力。 整合位点的遗传图可以鉴定来自转基因植物的植物。 这种方法还揭示了支持高水平基因表达的植物染色体上的遗传基因座，并且可以用作其他感兴趣的基因表达的整合位点。

公开(公告)号：US4466216A

公开(公告)日：1984-08-21

申请号：US396197

申请日：1982-07-08

申请人： John A. Howard

发明人： John A. Howard

IPC分类号： A01H4/00 ,  A01G1/00

CPC分类号： A01H4/005

摘要： Propagation of plants, particularly solanaceous plants, from tissue cultures is improved by the presence of exogenous carbon dioxide in the atmosphere.

摘要翻译： 植物，特别是茄科植物从组织培养物的繁殖通过在大气中存在外源性二氧化碳而得到改善。

公开(公告)号：US20090205086A1

公开(公告)日：2009-08-13

申请号：US12422035

申请日：2009-04-10

申请人： Elizabeth E. Hood ,  John A. Howard

发明人： Elizabeth E. Hood ,  John A. Howard

IPC分类号： A01H5/00

CPC分类号： C12N15/8242 ,  C12N15/8246 ,  C12N15/8257

摘要： Expression of recombinant polysaccharide degrading enzymes in plants is described. In one embodiment, expression of the enzyme is preferentially directed to the seed of the plant. Expression may also be preferentially targeted to specific locations within the plant cell. Expression of cellulases in corn is shown. The result is the capacity to produce polysaccharide degrading enzymes in plants at commercially acceptable levels in a reliable manner. Methods of using same in production of ethanol is also described, including use of the plant-produced enzymes in the ethanol production process.

摘要翻译： 描述了重组多糖降解酶在植物中的表达。 在一个实施方案中，酶的表达优先针对植物的种子。 表达也可以优先靶向植物细胞内的特定位置。 显示玉米纤维素酶的表达。 结果是以可靠的方式以商业上可接受的水平在植物中产生多糖降解酶的能力。 还描述了在乙醇生产中使用其的方法，包括在乙醇生产过程中使用植物生产的酶。

公开(公告)号：US07179961B2

公开(公告)日：2007-02-20

申请号：US10219122

申请日：2002-08-15

申请人： John A. Howard

发明人： John A. Howard

IPC分类号： C12N15/82 ,  C12P21/00

CPC分类号： C07K14/415 ,  C12N15/8234 ,  C12N15/8257

摘要： A method for extraction of heterologous protein from monocotyledonous plant seed comprises extracting the germ portion of the seed and extracting and purifying the protein from the germ. Enhanced expression in the germ is provided, and allows for improved efficiency in production, and cost savings. Directing expression to the germ portion further increases expression levels of the protein. The ubiquitin promoter preferentially directs expression to the germ portion of plant seed.

摘要翻译： 从单子叶植物种子提取异源蛋白质的方法包括提取种子的胚芽部分并从细菌中提取和纯化蛋白质。 提供了细菌的增强表达，并且可以提高生产效率和节约成本。 将表达指导到胚芽部分进一步增加蛋白质的表达水平。 泛素启动子优先将表达指导到植物种子的胚芽部分。