Commercial production of proteases in plants
    4.
    发明授权
    Commercial production of proteases in plants 失效
    在植物中商业化生产蛋白酶

    公开(公告)号:US6087558A

    公开(公告)日:2000-07-11

    申请号:US120582

    申请日:1998-07-22

    CPC分类号: C12N9/6427 C12N15/8257

    摘要: Production of proteases in plants is set forth, whereby heterologous DNA encoding the protease is introduced into the plant and expression of the protein achieved. By such methods, expression is achieved in plants wherein the plant cell is not damaged, the protein can be recovered without contamination by other proteases, and can be expressed at levels such that commercial production of the enzyme is obtained. Expression levels can be at 0.1% of total soluble protein of the plant, or higher.

    摘要翻译: 阐述植物中蛋白酶的产生,由此将编码蛋白酶的异源DNA引入植物中并实现蛋白质的表达。 通过这样的方法,在植物细胞未受损害的植物中实现表达,可以在不受其它蛋白酶污染的情况下回收蛋白质,并且可以以获得酶的商业生产的水平来表达。 表达水平可以在植物总可溶性蛋白质的0.1%以上。

    Commercial production of laccase in plants
    7.
    发明授权
    Commercial production of laccase in plants 失效
    在植物中商业生产漆酶

    公开(公告)号:US06800792B1

    公开(公告)日:2004-10-05

    申请号:US09786960

    申请日:2001-03-12

    IPC分类号: C12N1509

    摘要: Expression of laccase in plants at commercial levels of production is provided. The laccase gene is preferably operably linked with promoter sequences preferentially directing expression of laccase to the seed of the plant, and may additionally include sequences directing expression to the plant cell wall. Methods of improving the process of introducing DNA into plants via Agrobacterium are also provided.

    摘要翻译: 提供漆酶在商业生产水平的植物中的表达。 漆酶基因优选与优先引导漆酶表达的植物的启动子序列可操作地连接,并且还可以包括将表达指导到植物细胞壁的序列。 还提供了通过农杆菌改进将DNA导入植物的过程的方法。

    Methods of commercial production and extraction of protein from seed
    10.
    发明授权
    Methods of commercial production and extraction of protein from seed 有权
    从种子商业生产和提取蛋白质的方法

    公开(公告)号:US06504085B1

    公开(公告)日:2003-01-07

    申请号:US09403686

    申请日:1999-09-07

    申请人: John A. Howard

    发明人: John A. Howard

    IPC分类号: A01H500

    摘要: A method for extraction of heterologous protein from plant seed comprises extracting the germ portion of the seed and extracting and purifying the protein from the germ. Enhanced expression in the germ is provided, and allows for improved efficiency in production, and cost savings. Directing expression to the germ portion further increases expression levels of the protein. The ubiquitin promoter preferentially directs expression to the germ portion of plant seed.

    摘要翻译: 从植物种子提取异源蛋白质的方法包括提取种子的胚芽部分并从细菌中提取和纯化蛋白质。 提供了细菌的增强表达,并且可以提高生产效率和节约成本。 将表达引导到胚芽部分进一步增加蛋白质的表达水平。 泛素启动子优先将表达指导到植物种子的胚芽部分。