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公开(公告)号：US5824870A
公开(公告)日：1998-10-20
申请号：US554161
申请日：1995-11-06
申请人： Chris Baszczynski , Thomas Czapla , Elizabeth Hood , Terry EuClaire Meyer , David Peterson , A. Gururaj Rao , James C. Register, III , Derrick Witcher , John A. Howard
发明人： Chris Baszczynski , Thomas Czapla , Elizabeth Hood , Terry EuClaire Meyer , David Peterson , A. Gururaj Rao , James C. Register, III , Derrick Witcher , John A. Howard
CPC分类号： C07K14/8117 , C12N15/8257 , C12N15/8286
摘要： A method for commercial production of aprotinin entails heterologous expression of the protein in plants, preferably at a level such that aprotinin accounts for at least 0.1% of the total extracted protein. An aprotinin-expressing plant also has potential of increased insecticidal resistance by virtue of producing the protein. A genetic map of the integration locus allows identification of plants derived from the transgenic plant. This approach also reveals genetic loci on a plant chromosome that support high levels of gene expression and can be used as site of integration for expression of other genes of interest.
摘要翻译： 抑肽酶的商业生产方法需要在植物中异源表达蛋白质，优选地使得抑肽酶占总提取蛋白质的至少0.1％。 表达抑肽酶的植物还具有通过产生蛋白质而具有增加的杀虫抗性的潜力。 整合位点的遗传图可以鉴定来自转基因植物的植物。 这种方法还揭示了支持高水平基因表达的植物染色体上的遗传基因座，并且可以用作其他感兴趣的基因表达的整合位点。
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公开(公告)号：US06946589B1
公开(公告)日：2005-09-20
申请号：US09478567
申请日：2000-01-06
IPC分类号： A01H1/00 , A01H5/00 , C07K14/415 , C12N5/04 , C12N15/10 , C12N15/82 , C40B40/02 , C12N15/00 , A01H5/10 , C12N15/29
CPC分类号： C12N15/1058 , C12N15/10 , C12N15/102 , C12N15/1034 , C12N15/1037 , C12N15/8243 , C12N15/8251 , C12N15/8253 , C12N15/8254 , C40B40/02
摘要： Methods and compositions for altering amino acid composition of a protein of interest are provided, particularly proteins whose three-dimensional structure is unknown. The method comprises creating interacting molecules to the native protein and selecting for engineered proteins which retain the native conformation by antibody binding. In this manner, the levels of essential amino acids in a protein can be increased yet the biological activity of the protein maintained. Also provided is an exemplary plant protein—Glycine max vegetative storage protein (VSP)—in which methionine levels have been increased.
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公开(公告)号：US07053282B1
公开(公告)日：2006-05-30
申请号：US09020716
申请日：1998-02-09
申请人： Rudolf Jung , Larry R. Beach , Virginia M. Dress , A. Gururaj Rao , Jerome P. Ranch , David S. Ertl , Regina K. Higgins
发明人： Rudolf Jung , Larry R. Beach , Virginia M. Dress , A. Gururaj Rao , Jerome P. Ranch , David S. Ertl , Regina K. Higgins
CPC分类号： C07K14/811 , C07K14/415 , C12N15/8234 , C12N15/8251 , C12N15/8253 , C12N15/8254
摘要： The present invention provides a plant seed the endosperm of which is characterized as having an elevated level of a preselected amino acid. The present invention also provides expression cassettes, vectors, plants, plant cells and a method for enhancing the nutritional value of seeds.
摘要翻译： 本发明提供一种植物种子，其胚乳的特征在于具有升高的预选氨基酸水平。 本发明还提供了表达盒，载体，植物，植物细胞和增强种子营养价值的方法。
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4.发明申请公开(公告)号：US20050223424A1
公开(公告)日：2005-10-06
申请号：US11145160
申请日：2005-06-03
CPC分类号： C12N15/1058 , C12N15/10 , C12N15/102 , C12N15/1034 , C12N15/1037 , C12N15/8243 , C12N15/8251 , C12N15/8253 , C12N15/8254 , C40B40/02
摘要： Methods and compositions for altering amino acid composition of a protein of interest are provided, particularly proteins whose three-dimensional structure is unknown. The method comprises creating interacting molecules to the native protein and selecting for engineered proteins which retain the native conformation by antibody binding. In this manner, the levels of essential amino acids in a protein can be increased yet the biological activity of the protein maintained.
摘要翻译： 提供了用于改变目的蛋白质的氨基酸组成的方法和组合物，特别是其三维结构未知的蛋白质。 该方法包括产生与天然蛋白质相互作用的分子，并选择通过抗体结合保留天然构象的工程化蛋白质。 以这种方式，可以增加蛋白质中必需氨基酸的水平，而保持蛋白质的生物活性。
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公开(公告)号：US06905877B1
公开(公告)日：2005-06-14
申请号：US09478598
申请日：2000-01-06
CPC分类号： C12N15/1058 , C12N15/10 , C12N15/102 , C12N15/1034 , C12N15/1037 , C12N15/8243 , C12N15/8251 , C12N15/8253 , C12N15/8254 , C40B40/02
摘要： Methods and compositions for altering amino acid composition of a protein of interest are provided, particularly proteins whose three-dimensional structure is unknown. The method comprises creating interacting molecules to the native protein and selecting for engineered proteins which retain the native conformation by antibody binding. In this manner, the levels of essential amino acids in a protein can be increased yet the biological activity of the protein maintained. Also provided is an exemplary plant protein—Glycine max vegetative storage protein (VSP)—in which methionine levels have been increased.
摘要翻译： 提供了用于改变目的蛋白质的氨基酸组成的方法和组合物，特别是其三维结构未知的蛋白质。 该方法包括产生与天然蛋白质相互作用的分子，并选择通过抗体结合保留天然构象的工程化蛋白质。 以这种方式，可以增加蛋白质中必需氨基酸的水平，而保持蛋白质的生物活性。 还提供了示例性的植物蛋白质 - 大豆营养物质储存蛋白（VSP） - 其中甲硫氨酸水平已经增加。
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公开(公告)号：US5464944A
公开(公告)日：1995-11-07
申请号：US81509
申请日：1993-06-21
申请人： A. Gururaj Rao , Lingxiu Zhong
发明人： A. Gururaj Rao , Lingxiu Zhong
CPC分类号： C07K7/08 , A01N47/44 , C12N15/8282
摘要： Two synthetic polypeptides provide cell-expressible antifungal activity.
摘要翻译： 两种合成多肽提供细胞表达的抗真菌活性。
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公开(公告)号：USRE39562E1
公开(公告)日：2007-04-10
申请号：US10010709
申请日：2001-11-05
申请人： A. Gururaj Rao , Larry R. Beach
发明人： A. Gururaj Rao , Larry R. Beach
CPC分类号： C12N15/8251 , A01N65/44 , C07K14/415 , C12N15/8254 , C12N15/8282
摘要： Derivatives of α-hordothionin made by position-specific substitution with lysine residues provide lysine enrichment while retaining the antifungal activity of the parent compound.
摘要翻译： 通过用赖氨酸残基的位置特异性取代制备的α-硫蛋白的衍生物提供赖氨酸富集，同时保留母体化合物的抗真菌活性。
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8.发明授权
公开(公告)号：US6015941A
公开(公告)日：2000-01-18
申请号：US962034
申请日：1997-10-31
申请人： A. Gururaj Rao
发明人： A. Gururaj Rao
CPC分类号： C12N15/8281 , A01N63/02 , C07K14/43509 , C07K7/08 , C12N15/8282
摘要： Compositions comprising antimicrobial peptides are provided. The peptides are derivatives of tachyplesin having amino acid substitutions in which the four cysteine residues are replaced with hydrophobic amino acids. The compositions are useful in methods of the control of fungal and bacterial activity in agricultural and medical applications. Methods for making and using the compounds of the invention are additionally provided.
摘要翻译： 提供了包含抗微生物肽的组合物。 该肽是具有氨基酸取代的tachyplesin的衍生物，其中四个半胱氨酸残基被疏水性氨基酸取代。 该组合物可用于在农业和医学应用中控制真菌和细菌活性的方法中。 另外提供了制备和使用本发明化合物的方法。
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公开(公告)号：US5990389A
公开(公告)日：1999-11-23
申请号：US838763
申请日：1997-04-10
申请人： A. Gururaj Rao , Larry Beach
发明人： A. Gururaj Rao , Larry Beach
CPC分类号： C12N15/8251 , A01N65/44 , C07K14/415 , C12N15/8254 , C12N15/8282
摘要： Derivatives of .alpha.-hordothionin made by position-specific substitution with lysine residues provide lysine enrichment while retaining the antifungal activity of the parent compound.
摘要翻译： 通过用赖氨酸残基的位置特异性取代制备的α-硫代硫酸的衍生物提供赖氨酸富集，同时保留母体化合物的抗真菌活性。
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公开(公告)号：US5607914A
公开(公告)日：1997-03-04
申请号：US179632
申请日：1994-01-07
申请人： A. Gururaj Rao , Lingxiu Zhong
发明人： A. Gururaj Rao , Lingxiu Zhong
IPC分类号： A01N63/02 , A61K38/00 , C07K7/06 , C07K7/08 , C07K14/00 , C07K14/415 , C07K14/435 , C12N15/82 , A61K38/04 , A61K38/16 , C07K7/00
CPC分类号： C07K7/08 , A01N63/02 , C07K14/001 , C07K14/415 , C07K14/43563 , C07K7/06 , C12N15/8281 , C12N15/8282 , A61K38/00
摘要： Synthetic polypeptides exhibiting amphipathic alpha-helices provide cell-expressible antimicrobial activity.
摘要翻译： 显示两亲性α-螺旋的合成多肽提供细胞表达的抗微生物活性。
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