Virus-resistant transgenic plants expressing L3
    7.
    发明申请
    Virus-resistant transgenic plants expressing L3 有权
    表达L3的病毒抗性转基因植物

    公开(公告)号:US20050114927A1

    公开(公告)日:2005-05-26

    申请号:US10636386

    申请日:2003-08-07

    摘要: Disclosed are transgenic plants containing an exogenous nucleic acid encoding an L3 protein. The plant exhibits increased resistance to viruses and/or fungi that infect plants. The L3 proteins include wild-type proteins, spontaneously occurring mutants and non-naturally occurring L3 mutants. Also disclosed are methods of reducing the toxicity of single-chain ribosome inhibitory proteins in cells, e.g., yeast, plant and animal cells, by co-administering the L3 protein with the RIP. Further disclosed are non-naturally occurring L3 mutants that (a) substantially fail to bind single-chain RIPs that bind endogenous L3 proteins, (b) are unable to maintain M1 killer virus, (c) promote altered programmed ribosomal frameshift efficiency, (d) exhibit resistance to peptidyltransferase inhibitors, and combinations of any of (a)-(d).

    摘要翻译: 公开了含有编码L3蛋白的外源核酸的转基因植物。 植物对感染植物的病毒和/或真菌的抗性增加。 L3蛋白包括野生型蛋白,自发发生的突变体和非天然存在的L3突变体。 还公开了通过将L3蛋白与RIP共同施用来降低单链核糖体抑制蛋白在细胞例如酵母,植物和动物细胞中的毒性的方法。 进一步披露的是非天然存在的L3突变体,其(a)基本上不能结合结合内源性L3蛋白的单链RIP,(b)不能维持M1杀伤病毒,(c)促进改变的程序化核糖体移码效率,(d )表现出对肽基转移酶抑制剂的抗性,以及(a) - (d)中任何一种的组合。