公开(公告)号：US08216780B2

公开(公告)日：2012-07-10

申请号：US12476796

申请日：2009-06-02

申请人： Jonathan Drew Smith ,  Breanna Leigh Dreiling ,  Breanna Christine Smith ,  John H. Wheeler

发明人： Jonathan Drew Smith ,  Breanna Leigh Dreiling ,  Breanna Christine Smith ,  John H. Wheeler

IPC分类号： C12Q1/70 ,  C12Q1/02

CPC分类号： C12Q1/04 ,  G01N33/54326 ,  G01N33/56911

摘要： A method of determining the presence or absence of a target microorganism in a sample to be tested, the method comprising: (a) combining with the sample an amount of bacteriophage capable of attaching to the target microorganism to create a bacteriophage-exposed sample; (b) providing conditions to the bacteriophage-exposed sample sufficient to allow the bacteriophage to attach to the target microorganism while inhibiting phage replication in a potentially cross-reactive, non-target microorganism; and (c) assaying the bacteriophage-exposed sample to detect the presence or absence of a bacteriophage marker to determine the presence or absence of the target microorganism; wherein the amount of the bacteriophage is between 10% to 70% of the threshold number of bacteriophage that the assay can detect, or between 1×106 pfu/mL and 7×106 pfu/mL.

摘要翻译： 一种确定待测试样品中目标微生物的存在或不存在的方法，所述方法包括：（a）与样品结合能够附着于靶微生物以产生噬菌体暴露样品的噬菌体数量; （b）向噬菌体暴露的样品提供足以允许噬菌体附着于靶微生物同时抑制潜在交叉反应性非目标微生物中的噬菌体复制的条件; 和（c）测定噬菌体暴露的样品以检测噬菌体标志物的存在或不存在以确定靶微生物的存在或不存在; 其中噬菌体的量在测定可检测的噬菌体阈值的10％至70％之间，或1×10 6 pfu / mL至7×10 6 pfu / mL之间。

公开(公告)号：US4179856A

公开(公告)日：1979-12-25

申请号：US865708

申请日：1977-12-29

申请人： John H. Wheeler

发明人： John H. Wheeler

IPC分类号： B24D99/00 ,  B24B19/00 ,  B24D3/22

CPC分类号： B24D99/00 ,  Y10T29/4506

摘要： A sleeve assembly for sanding a piston rod of a hydraulic cylinder or the like comprises a rigid annular outer member, a resilient annular intermediate member adhesively secured to the inner surface of the outer member, and an abrasive annular inner member adhesively secured to the inner surface of the intermediate member for interference contact with the piston rod. The sections of the annular members each have complementary nonaxial end configurations to prevent scoring of the piston rod during axial movement. Clamp means releasably engages the members around the piston rod. In some embodiments, bracket or angle bar means are utilized to releasably secure the sleeve assembly to structure at the end of the cylinder. In another embodiment, the sleeve assembly is releasably clamped directly around the end of the hydraulic cylinder.

摘要翻译： 用于对液压缸等的活塞杆进行打磨的套筒组件包括刚性环形外部构件，粘合地固定到外部构件的内表面的弹性环形中间构件，以及粘结地固定到内部表面的研磨性环形内部构件 用于与活塞杆干涉接触。 环形构件的各个部分各自具有互补的非轴端构造，以防止在轴向移动期间活塞杆的刻痕。 夹紧装置可释放地接合活塞杆周围的构件。 在一些实施例中，支架或角度杆装置用于将套筒组件可释放地固定到气缸端部的结构。 在另一个实施例中，套筒组件可释放地直接夹在液压缸的端部周围。

公开(公告)号：US4225014A

公开(公告)日：1980-09-30

申请号：US964154

申请日：1978-11-28

申请人： John H. Wheeler

发明人： John H. Wheeler

IPC分类号： B66B9/04 ,  B66B9/00

CPC分类号： B66B9/04 ,  Y10T403/45

摘要： An elevator connection (22) is provided for joining an elevator car (10) to a hydraulic piston (20) which raises and lowers the elevator car (10). Misalignment between the hydraulic piston (20) and guide rails (14) of an elevator shaft (12) is accommodated by a washer assembly (28) and cushion assembly (30) disposed between a stud bolt (32) and the top of the hydraulic piston (20). Washer assembly (28) includes an elastomeric disc (34) together with springs (36) which enclose a tubular washer (42) having a convex lower end surface. Cushion assembly (30) comprises an elastomeric disc (46) with springs (48) on either side thereof and enclosing a wobble tube (54) having convex end surfaces (56, 58). The elevator car (10) is held securely to the hydraulic piston (20) but is permitted to offset slightly from the piston (20) and tilt to accommodate misalignment of elevator guide rails (14) with the hydraulic piston (20).

摘要翻译： 提供一种用于将电梯轿厢（10）连接到升高和降低电梯轿厢（10）的液压活塞（20）的电梯连接件（22）。 液压活塞（20）和升降机井道（12）的导轨（14）之间的不对准由设置在双头螺栓（32）和液压顶部（32）之间的垫圈组件（28）和缓冲组件（30） 活塞（20）。 洗涤器组件（28）包括弹性体盘（34）以及弹簧（36），弹簧围绕具有凸下端表面的管状垫圈（42）。 缓冲组件（30）包括在其任一侧上具有弹簧（48）的弹性体盘（46），并且包围具有凸出端表面（56,58）的摆动管（54）。 电梯轿厢（10）被牢固地保持在液压活塞（20）上，但被允许从活塞（20）稍微偏移并且倾斜以适应电梯导轨（14）与液压活塞（20）的不对准。

公开(公告)号：US4123990A

公开(公告)日：1978-11-07

申请号：US850654

申请日：1977-11-11

申请人： John H. Wheeler

发明人： John H. Wheeler

IPC分类号： B24D99/00 ,  B05C11/00

CPC分类号： B24D99/00 ,  Y10T29/4506

摘要： A sleeve assembly for sanding, rust removing, and pit filling on a piston rod of a hydraulic cylinder or the like includes a sanding component, a rust removing component and a pit filling component. The sanding component comprises a rigid annular outer member, a resilient annular intermediate member adhesively secured to the inner surface of the outer member, and an abrasive annular inner member adhesively secured to the inner surface of the intermediate member and in interference contact with the piston rod. The rust component comprises an annular upper ring member composed of a rigid material and an elastomeric annular lower ring member having brass or bronze particles imbedded therein. The pit filling component comprises an annular upper ring composed of a rigid material and an elastomeric annular lower ring member having lead particles imbedded therein. Clamp means releasably engages the members of each of the components about the piston rod.

摘要翻译： 用于在液压缸等的活塞杆上进行砂磨，除锈和凹坑填充的套筒组件包括砂磨部件，除锈部件和凹坑填充部件。 打磨组件包括刚性环形外部构件，粘合地固定到外部构件的内表面的弹性环形中间构件和粘结地固定到中间构件的内表面并与活塞杆干涉接触的研磨环形内部构件 。 防锈部件包括由刚性材料构成的环形上环构件和嵌入其中的黄铜或青铜颗粒的弹性体环形下环构件。 坑填充部件包括由刚性材料构成的环形上环和具有嵌入其中的铅颗粒的弹性体环形下环构件。 夹紧装置可释放地接合围绕活塞杆的每个部件的构件。

公开(公告)号：US20090286232A1

公开(公告)日：2009-11-19

申请号：US12476796

申请日：2009-06-02

申请人： Jonathan Drew Smith ,  Breanna Leigh Dreiling ,  Breanna Christine Smith ,  John H. Wheeler

发明人： Jonathan Drew Smith ,  Breanna Leigh Dreiling ,  Breanna Christine Smith ,  John H. Wheeler

IPC分类号： C12Q1/70

CPC分类号： C12Q1/04 ,  G01N33/54326 ,  G01N33/56911

摘要： A method of determining the presence or absence of a target microorganism in a sample to be tested, the method comprising: (a) combining with the sample an amount of bacteriophage capable of attaching to the target microorganism to create a bacteriophage-exposed sample; (b) providing conditions to the bacteriophage-exposed sample sufficient to allow the bacteriophage to attach to the target microorganism while inhibiting phage replication in a potentially cross-reactive, non-target microorganism; and (c) assaying the bacteriophage-exposed sample to detect the presence or absence of a bacteriophage marker to determine the presence or absence of the target microorganism; wherein the amount of the bacteriophage is between 10% to 70% of the threshold number of bacteriophage that the assay can detect, or between 1×106pfu/mL and 7×106pfu/mL.

摘要翻译： 一种确定待测试样品中目标微生物的存在或不存在的方法，所述方法包括：（a）与样品结合能够附着于靶微生物以产生噬菌体暴露样品的噬菌体数量; （b）向噬菌体暴露的样品提供足以允许噬菌体附着于靶微生物同时抑制潜在交叉反应性非目标微生物中的噬菌体复制的条件; 和（c）测定噬菌体暴露的样品以检测噬菌体标志物的存在或不存在以确定靶微生物的存在或不存在; 其中所述噬菌体的量为所述测定可检测的噬菌体阈值的10％至70％，或1×10 6 pfu / mL至7×10 6 pfu / mL之间。

公开(公告)号：US5426270A

公开(公告)日：1995-06-20

申请号：US87962

申请日：1993-07-06

申请人： John H. Wheeler ,  Jimmy P. Frentzos

发明人： John H. Wheeler ,  Jimmy P. Frentzos

IPC分类号： F16L55/027 ,  F16L55/033 ,  F16L55/02

CPC分类号： F16L55/0332 ,  F16L55/02763

摘要： A muffler for reducing fluid flow noise in a hydraulic system comprises a cylindrical elastomer insert having multiple reinforced flow ports with different diameter porting, internal air chambers, and cast from an elastomer mixed with hollow glass spheres to form an insert of 40 percent air by volume. The insert is placed within a housing and connected to the hydraulic pipe assembly for receiving fluid under pressure and diverting it through the multiple flow ports of the insert wherein flow sounds, pump surges, and flushing noises of the hydraulic system are muffled by the disruption of the flow through the flow ports, the absorption by the internal air chambers, and the absorption by microspheres created by the hollow glass spheres in the elastomer insert.

公开(公告)号：US4336946A

公开(公告)日：1982-06-29

申请号：US185205

申请日：1980-09-08

申请人： John H. Wheeler

发明人： John H. Wheeler

IPC分类号： F16J15/32 ,  F16J15/24

CPC分类号： F16J15/32 ,  Y10S277/91

摘要： An elastomeric O-ring seal (10) for sealing between a housing (20) and a shaft (22). The seal (10) defines an inner sealing surface (12) and an outer sealing surface (14) having a generally uniform non-deformed radius of curvature in cross section. An upper protrusion (16) and a lower protrusion (18) extend outward of a circle defined by this radius in cross section to prevent the seal (10) from rolling when placed in sealing engagement with the housing (20) and shaft (22). This reduces the wear on the seal (10) and permits a longer surface life.

摘要翻译： 用于在壳体（20）和轴（22）之间进行密封的弹性体O形密封圈（10）。 密封件（10）限定了内部密封表面（12）和外部密封表面（14），其在横截面上具有大致均匀的不变形曲率半径。 上部突起（16）和下部突起（18）从由该半径形成的圆形横截面向外延伸，以防止密封件（10）在与壳体（20）和轴（22）密封接合时滚动， 。 这减少了密封件（10）上的磨损并且允许更长的表面寿命。

公开(公告)号：US4089137A

公开(公告)日：1978-05-16

申请号：US721984

申请日：1976-09-10

申请人： John H. Wheeler

发明人： John H. Wheeler

IPC分类号： B24D99/00 ,  B24B19/00 ,  B24D3/22

CPC分类号： B24D99/00 ,  Y10T29/4506

摘要： A sleeve assembly for sanding, rust removing, and pit filling on a piston rod of a hydraulic cylinder or the like includes a sanding component, a rust removing component and a pit filling component. The sanding component comprises a rigid annular outer member, a resilient annular intermediate member adhesively secured to the inner surface of the outer member, and an abrasive annular inner member adhesively secured to the inner surface of the intermediate member and in interference contact with the piston rod. The rust removing component comprises an annular upper ring member composed of a rigid material and an elastomeric annular lower C-ring member having brass or bronze particles embedded therein. The pit filling component comprises an annular upper ring member composed of a rigid material and an elastomeric annular lower C-ring member having lead particles imbedded therein. Clamp means releasably engages the members of each of the components about the piston rod.

摘要翻译： 用于在液压缸等的活塞杆上进行砂磨，除锈和凹坑填充的套筒组件包括砂磨部件，除锈部件和凹坑填充部件。 打磨组件包括刚性环形外部构件，粘合地固定到外部构件的内表面的弹性环形中间构件和粘结地固定到中间构件的内表面并与活塞杆干涉接触的研磨环形内部构件 。 除锈部件包括由刚性材料构成的环形上环构件和嵌入其中的黄铜或青铜颗粒的弹性体环形下C形环构件。 坑填充部件包括由刚性材料构成的环形上环构件和具有嵌入其中的铅颗粒的弹性环形下C形环构件。 夹紧装置可释放地接合围绕活塞杆的每个部件的构件。

公开(公告)号：US20090246752A1

公开(公告)日：2009-10-01

申请号：US12346656

申请日：2008-12-30

申请人： Kent Voorhees ,  Jon Rees ,  John H. Wheeler ,  Angelo Madonna

发明人： Kent Voorhees ,  Jon Rees ,  John H. Wheeler ,  Angelo Madonna

IPC分类号： C12Q1/70

CPC分类号： C12Q1/04 ,  G01N33/54326 ,  G01N33/56911

摘要： A method for detecting one or more target bacteria in a raw sample where: 1) bacteriophage(s) specific to each target bacterium are added to the raw sample, 2) the test sample is incubated, and 3) the test sample is tested for the presence of each phage in sufficient numbers to indicate the presence of the associated target bacteria in the raw sample. In one embodiment, each phage is initially added to the raw sample in concentrations below the detection limit of the final phage detection process. In another embodiment, the parent phages are tagged in such a way that they can be separated from the progeny phage prior to the detection process. Preferred phage detection processes are immunoassay methods utilizing antibodies that bind specifically to each phage. Antibodies can be used that bind to the protein capsid of the phage. Alternatively, the phage can by dissociated after the incubation process and the sample tested for the presence of individual capsid proteins or phage nucleic acids. The invention can be used to test target bacteria for antibiotic resistance.

摘要翻译： 一种用于检测原料样品中的一种或多种目标细菌的方法，其中：1）将每种靶细菌特异性的噬菌体加入到原始样品中，2）将测试样品孵育，并且3）测试样品 每个噬菌体的存在足够数量以指示原始样品中相关靶细菌的存在。 在一个实施方案中，每个噬菌体最初以低于最终噬菌体检测过程的检测限的浓度加入到原始样品中。 在另一个实施方案中，母体噬菌体的标记方式使得它们可以在检测过程之前与后代噬菌体分离。 优选的噬菌体检测方法是利用与每个噬菌体特异性结合的抗体的免疫测定方法。 可以使用结合噬菌体的蛋白质衣壳的抗体。 或者，噬菌体可以通过在孵育过程之后解离并且样品测试个体衣壳蛋白或噬菌体核酸的存在。 本发明可用于测试目标细菌的抗生素耐药性。

公开(公告)号：US20070178450A1

公开(公告)日：2007-08-02

申请号：US11698673

申请日：2007-01-25

申请人： John H. Wheeler ,  Jon C. Rees ,  Gregory S. Gaisford

发明人： John H. Wheeler ,  Jon C. Rees ,  Gregory S. Gaisford

IPC分类号： C12Q1/70 ,  C12Q1/68

CPC分类号： G01N33/56911 ,  C12Q1/06 ,  C12Q1/18

摘要： A predetermined amount of parent bacteriophage capable of infecting a target microorganism is added to a sample to create a bacteriophage-exposed sample; the sample is incubated for a defined incubation time and assayed to determine the level of a bacteriophage or bacterial marker in the sample; and if the measured marker level has increased, then the initial concentration of the microorganism exceeds a specific threshold value. An antibiotic in different concentrations is added to different and separate portions of the sample and tested to determine if the bacteriophage marker is present and thereby determine the Minimum Inhibitory Concentration (MIC) of a given antibiotic. The antibiotic preferably is an antibiotic that inhibits DNA replication or protein synthesis.

摘要翻译： 将能够感染靶微生物的预定量的亲本噬菌体添加到样品中以产生暴露于噬菌体的样品; 将样品温育定义的孵育时间并测定以测定样品中噬菌体或细菌标记物的水平; 并且如果测量的标记水平增加，则微生物的初始浓度超过特定的阈值。 将不同浓度的抗生素加入样品的不同和分离部分，并进行测试以确定是否存在噬菌体标记物，从而确定给定抗生素的最低抑制浓度（MIC）。 抗生素优选是抑制DNA复制或蛋白质合成的抗生素。