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公开(公告)号：US06541622B1
公开(公告)日：2003-04-01
申请号：US09632313
申请日：2000-08-04
IPC分类号： C07H2104
摘要： The present invention provides the promoter clone discovery of an alpha-amylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated alpha-amylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.
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公开(公告)号：US06551798B2
公开(公告)日：2003-04-22
申请号：US09921942
申请日：2001-08-02
IPC分类号： C12N1563
摘要： The present invention provides the promoter clone discovery of an alpha-amylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated alpha-amylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.
摘要翻译： 本发明提供了使用酵母菌株施氏葡萄球菌（Schwanniomyces castellii）的淀粉的α-淀粉酶基因的启动子克隆发现。 分离的α-淀粉酶启动子是可诱导的启动子，可以调节淀粉培养基中的强基因表达。
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3.发明授权Isolated yeast promoter sequence and a method of regulated heterologous expression 失效分离的酵母启动子序列和调节异源表达的方法公开(公告)号：US06900305B2
公开(公告)日：2005-05-31
申请号：US09921944
申请日：2001-08-02
CPC分类号： C07K14/39
摘要： The present invention provides the promoter clone discovery of a glucoamylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated glucoamylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.
摘要翻译： 本发明提供了使用酵母菌株施氏硬糖酵母（Schwanniomyces castellii）的淀粉的葡糖淀粉酶基因的启动子克隆发现。 分离的葡糖淀粉酶启动子是可以调节淀粉培养基中强基因表达的诱导型启动子。
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公开(公告)号：US06355864B1
公开(公告)日：2002-03-12
申请号：US09417019
申请日：1999-10-12
申请人： Lifang Shi , Ziyu Dai , Johnway Gao , Brian S. Hooker , Daniel B. Anderson
发明人： Lifang Shi , Ziyu Dai , Johnway Gao , Brian S. Hooker , Daniel B. Anderson
IPC分类号： C07H2104
CPC分类号： C12N15/8238 , C12N15/8216 , C12N15/8229 , C12N15/8237 , C12N15/8239
摘要： Synergistically functional, yet separable, cis-acting enhancer elements from the rpL34 promoter are disclosed. These enhancer elements of the instant invention may be used in combination with a plurality of promoters to increase gene expression without affecting the intrinsic specificity of the promoters. Also disclosed are methods for using the enhancer elements of the instant invention as well as vectors and transgenic plants comprising the enhancer elements.
摘要翻译： 公开了来自rpL34启动子的协同作用的，但可分离的顺式作用的增强子元件。 本发明的这些增强子元件可以与多个启动子组合使用以增加基因表达而不影响启动子的固有特异性。 还公开了使用本发明的增强子元件以及包含增强子元件的载体和转基因植物的方法。
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5.发明授权Isolation and characterization of the genomic DNA clones of ribosomal protein gene L25 in tobacco 失效烟草中核糖体蛋白基因L25的基因组DNA克隆的分离和表征公开(公告)号：US06783978B1
公开(公告)日：2004-08-31
申请号：US09714948
申请日：2000-11-20
申请人： Lifang Shi , Johnway Gao , Brian S. Hooker , Ziyu Dai
发明人： Lifang Shi , Johnway Gao , Brian S. Hooker , Ziyu Dai
IPC分类号： C12N1500
CPC分类号： C12N15/8234 , C12N15/8225
摘要： The promoter of the ribosomal protein gene L25 is operably linked to a structural gene. This chimeric gene is placed in an expression vector. The expression vector containing the chimeric gene is used to transform plants cells and plants. Seeds are obtained from the transformed plants. A product, such as a protein, encoded by the structural gene is isolated from the transformed plant cells and plants.
摘要翻译： 核糖体蛋白基因L25的启动子与结构基因可操作地连接。 将该嵌合基因置于表达载体中。 含有嵌合基因的表达载体用于转化植物细胞和植物。 从转化的植物获得种子。 从转化的植物细胞和植物中分离由结构基因编码的产物，如蛋白质。
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