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公开(公告)号：US20120320365A1
公开(公告)日：2012-12-20
申请号：US13597418
申请日：2012-08-29
申请人： Andrew P. Bartko , Johnway Gao
发明人： Andrew P. Bartko , Johnway Gao
CPC分类号： G01N1/30 , G01N1/312 , G01N35/00029 , Y10T436/2575
摘要： A sample is cleaned up for spectroscopic analysis by receiving a slide substrate having the sample thereon, fixing the sample to a substrate surface of the slide substrate by applying heat to the slide substrate for a predetermined heating time and incubating the sample on the slide substrate for a predetermined incubation time after fixing the sample to the slide substrate. The sample is further cleaned by washing the sample on the slide substrate after the sample has been incubated and drying the sample by applying heat to the slide substrate for a predetermined drying time, wherein the sample on the slide substrate after drying has retained particles of interest and interferant particles are removed from the substrate. A substrate is also provided for sample collection, which is culturable and Raman silent.
摘要翻译： 通过接收具有样品的载玻片基板将样品清理干净，用于将样品固定在载玻片基板的基板表面上，通过向滑动基板施加预定加热时间并将样品温育在载玻片基板上 在将样品固定到载玻片基板之后的预定孵育时间。 通过在将样品温育并将样品通过向滑动基底施加热量干燥样品进行预定的干燥时间之后，通过在载玻片基板上洗涤样品来进一步清洗样品，其中在干燥后的载玻片基板上的样品已经保留了感兴趣的颗粒 并且从衬底去除干燥剂颗粒。 还提供了用于样品收集的底物，其是可培养的和拉曼沉默的。
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2.发明申请Production of human coagulation factor VIII from plant cells and whole plants 审中-公开从植物细胞和整株植物生产人凝血因子VIII公开(公告)号：US20050060775A1
公开(公告)日：2005-03-17
申请号：US10863969
申请日：2004-06-08
申请人： Brian Hooker , Daniel Anderson , Johnway Gao , Ziyu Dai
发明人： Brian Hooker , Daniel Anderson , Johnway Gao , Ziyu Dai
CPC分类号： C12N15/8257
摘要： The invention includes methods for production of a polypeptide having factor VIII activity by introduction of a polynucleotide construct into a plant cell. The construct includes an encoding sequence for a polypeptide of coagulation factor VIII or a functional variant thereof. The plant cell is cultured or regenerated into a plant and the polypeptide or functional variant of factor VIII is expressed therein. The invention also includes vectors, plant cells, plant tissues, plants and seeds containing a polynucleotide sequence encoding a functional variant of human coagulation factor VIII. The invention further includes a recombinant DNA molecule having a promoter which is functional in plants operably linked to a coding sequence which codes for a polynucleotide having coagulation factor VIII activity.
摘要翻译： 本发明包括通过将多核苷酸构建体引入植物细胞来生产具有因子VIII活性的多肽的方法。 该构建体包含凝血因子VIII多肽或其功能变体的编码序列。 将植物细胞培养或再生成植物，并在其中表达因子VIII的多肽或功能变体。 本发明还包括含有编码人凝血因子VIII功能变体的多核苷酸序列的载体，植物细胞，植物组织，植物和种子。 本发明还包括具有启动子的重组DNA分子，该启动子在与可编码具有凝血因子VIII活性的多核苷酸的编码序列可操作地连接的植物中是功能性的。
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公开(公告)号：US06541622B1
公开(公告)日：2003-04-01
申请号：US09632313
申请日：2000-08-04
IPC分类号： C07H2104
摘要： The present invention provides the promoter clone discovery of an alpha-amylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated alpha-amylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.
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4.发明授权Promoter sequence of 3-phosphoglycerate kinase gene 2 of lactic acid-producing fungus rhizopus oryzae and a method of expressing a gene of interest in fungal species 失效产生乳酸的真菌根霉的3-磷酸甘油酸激酶基因2的启动子序列和在真菌种中表达感兴趣的基因的方法公开(公告)号：US06528636B1
公开(公告)日：2003-03-04
申请号：US09535851
申请日：2000-03-27
申请人： Johnway Gao , Rodney S. Skeen
发明人： Johnway Gao , Rodney S. Skeen
IPC分类号： C07H2104
CPC分类号： C12N9/1217 , C12N15/80
摘要： The present invention provides the promoter clone discovery of phosphoglycerate kinase gene 2 of a lactic acid-producing filamentous fungal strain, Rhizopus oryzae. The isolated promoter can constitutively regulate gene expression under various carbohydrate conditions. In addition, the present invention also provides a design of an integration vector for the transformation of a foreign gene in Rhizopus oryzae.
摘要翻译： 本发明提供了产生乳酸的丝状真菌菌株，米根霉（Rhizopus oryzae）的磷酸甘油酸激酶基因2的启动子克隆发现。 分离的启动子可以在各种碳水化合物条件下组成型调节基因表达。 此外，本发明还提供了用于在米根霉（Rhizopus oryzae）中外源基因转化的整合载体的设计。
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公开(公告)号：US09034278B2
公开(公告)日：2015-05-19
申请号：US13597418
申请日：2012-08-29
申请人： Andrew P. Bartko , Johnway Gao
发明人： Andrew P. Bartko , Johnway Gao
CPC分类号： G01N1/30 , G01N1/312 , G01N35/00029 , Y10T436/2575
摘要： A sample is cleaned up for spectroscopic analysis by receiving a slide substrate having the sample thereon, fixing the sample to a substrate surface of the slide substrate by applying heat to the slide substrate for a predetermined heating time and incubating the sample on the slide substrate for a predetermined incubation time after fixing the sample to the slide substrate. The sample is further cleaned by washing the sample on the slide substrate after the sample has been incubated and drying the sample by applying heat to the slide substrate for a predetermined drying time, wherein the sample on the slide substrate after drying has retained particles of interest and interferant particles are removed from the substrate. A substrate is also provided for sample collection, which is culturable and Raman silent.
摘要翻译： 通过接收具有样品的载玻片基板将样品清理干净，用于将样品固定在载玻片基板的基板表面上，通过向滑动基板施加预定加热时间并将样品温育在载玻片基板上 在将样品固定到载玻片基板之后的预定孵育时间。 通过在将样品温育并将样品通过向滑动基底施加热量预定的干燥时间来干燥样品之后，通过在载玻片基板上洗涤样品来进一步清洗样品，其中在干燥后的载玻片基板上的样品已经保留了感兴趣的颗粒 并且从衬底去除干燥剂颗粒。 还提供了用于样品收集的底物，其是可培养的和拉曼沉默的。
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公开(公告)号：US07993585B2
公开(公告)日：2011-08-09
申请号：US11486939
申请日：2006-07-14
申请人： Rodney S. Black , Jeffrey P. Carpenter , Johnway Gao , Jerome U. Gilberry , John J. Kester , Clark A. Morrow
发明人： Rodney S. Black , Jeffrey P. Carpenter , Johnway Gao , Jerome U. Gilberry , John J. Kester , Clark A. Morrow
CPC分类号： G01N15/1463 , G01N2015/0261 , G01N2015/1488 , Y10T436/11 , Y10T436/115831
摘要： A biological and chemical detection system is provided that detects and identifies biological and/or chemical particulates of interest. The biological and chemical detection system comprises a collector, a first optical device, a second optical device and a processor. The collector is configured to deposit particulates drawn from a fluid stream onto a sample substrate to define a sample area. The first optical device derives first data relative to at least a portion of the sample area, which is analyzed to determine at least one field of view and/or specific target location. The second optical device then interrogates the sample area at each determined target location, e.g., using Raman spectroscopy, to produce interrogation data. The processor determines whether the sample area includes predetermined biological or chemical particulates of interest based upon an analysis of the interrogation data and triggers an event such as an alarm or message if the predetermined biological or chemical particulates of interest are identified.
摘要翻译： 提供了一种生物和化学检测系统，用于检测和识别感兴趣的生物和/或化学颗粒物。 生物和化学检测系统包括收集器，第一光学装置，第二光学装置和处理器。 收集器被配置为将从流体流中吸取的微粒沉积到样品基底上以限定样品区域。 第一光学装置相对于样本区域的至少一部分导出第一数据，其被分析以确定至少一个视场和/或特定目标位置。 然后，第二光学装置在每个确定的目标位置询问样品区域，例如使用拉曼光谱法，产生询问数据。 处理器基于询问数据的分析来确定样本区域是否包括预期的生物或化学颗粒物，并且如果识别出预定的感兴趣的生物或化学颗粒物，则触发诸如报警或消息的事件。
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7.发明授权Isolation and characterization of the genomic DNA clones of ribosomal protein gene L25 in tobacco 失效烟草中核糖体蛋白基因L25的基因组DNA克隆的分离和表征公开(公告)号：US06783978B1
公开(公告)日：2004-08-31
申请号：US09714948
申请日：2000-11-20
申请人： Lifang Shi , Johnway Gao , Brian S. Hooker , Ziyu Dai
发明人： Lifang Shi , Johnway Gao , Brian S. Hooker , Ziyu Dai
IPC分类号： C12N1500
CPC分类号： C12N15/8234 , C12N15/8225
摘要： The promoter of the ribosomal protein gene L25 is operably linked to a structural gene. This chimeric gene is placed in an expression vector. The expression vector containing the chimeric gene is used to transform plants cells and plants. Seeds are obtained from the transformed plants. A product, such as a protein, encoded by the structural gene is isolated from the transformed plant cells and plants.
摘要翻译： 核糖体蛋白基因L25的启动子与结构基因可操作地连接。 将该嵌合基因置于表达载体中。 含有嵌合基因的表达载体用于转化植物细胞和植物。 从转化的植物获得种子。 从转化的植物细胞和植物中分离由结构基因编码的产物，如蛋白质。
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公开(公告)号：US06355864B1
公开(公告)日：2002-03-12
申请号：US09417019
申请日：1999-10-12
申请人： Lifang Shi , Ziyu Dai , Johnway Gao , Brian S. Hooker , Daniel B. Anderson
发明人： Lifang Shi , Ziyu Dai , Johnway Gao , Brian S. Hooker , Daniel B. Anderson
IPC分类号： C07H2104
CPC分类号： C12N15/8238 , C12N15/8216 , C12N15/8229 , C12N15/8237 , C12N15/8239
摘要： Synergistically functional, yet separable, cis-acting enhancer elements from the rpL34 promoter are disclosed. These enhancer elements of the instant invention may be used in combination with a plurality of promoters to increase gene expression without affecting the intrinsic specificity of the promoters. Also disclosed are methods for using the enhancer elements of the instant invention as well as vectors and transgenic plants comprising the enhancer elements.
摘要翻译： 公开了来自rpL34启动子的协同作用的，但可分离的顺式作用的增强子元件。 本发明的这些增强子元件可以与多个启动子组合使用以增加基因表达而不影响启动子的固有特异性。 还公开了使用本发明的增强子元件以及包含增强子元件的载体和转基因植物的方法。
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9.发明申请公开(公告)号：US20120264178A1
公开(公告)日：2012-10-18
申请号：US13416911
申请日：2012-03-09
申请人： Dwight ANDERSON , Johnway Gao , Benjamin Levie
发明人： Dwight ANDERSON , Johnway Gao , Benjamin Levie
摘要： The present invention provides methods of producing pretreated lignocellulosic biomass combined with enzymes for the storage and transporation of the pretreated lignocellulosic biomass that may be used in biofuel and bioproduct production. The methods allows the coexistence of the pretreated lignocellulosic biomass and the enzymes during storage and transporation, the immediate hydrolysis of the pretreated lignocellulosic biomass to produce sugars, without further addition of enzymes, in a biofuel or bioproduct production site, the enhancement of the final hydrolytic activity of the pretreated lignocellulosic biomass, and/or the reduction in sensitivity of the inhibitors in the pretreated lignocellulosic biomass.
摘要翻译： 本发明提供了生产预处理的木质纤维素生物质与酶结合的方法，用于储存和运输可用于生物燃料和生物产物生产的预处理的木质纤维素生物质。 这些方法允许在储存和运输过程中预处理的木质纤维素生物质和酶的共存，预处理的木质纤维素生物质在生物燃料或生物产物生产位点中立即水解而不进一步加入酶以产生糖，增强最终的水解 预处理的木质纤维素生物质的活性和/或抑制剂在预处理的木质纤维素生物质中的敏感性的降低。
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公开(公告)号：US07518710B2
公开(公告)日：2009-04-14
申请号：US11486619
申请日：2006-07-14
申请人： Johnway Gao , Jerome U. Gilberry , William A. Ivancic , John J. Kester , Joseph A. McClintock , Clark A. Morrow , Jason A. Schaefer
发明人： Johnway Gao , Jerome U. Gilberry , William A. Ivancic , John J. Kester , Joseph A. McClintock , Clark A. Morrow , Jason A. Schaefer
CPC分类号： G01N15/1463 , G01N2015/0261 , G01N2015/1488 , Y10T436/11 , Y10T436/115831
摘要： A biological and chemical detection system is provided that detects and identifies biological and/or chemical particulates of interest. The biological and chemical detection system comprises a collector, a first optical device, a second optical device and a processor. The collector is configured to deposit particulates drawn from a fluid stream onto a sample substrate to define a sample area. The first optical device derives first data relative to at least a portion of the sample area, which is analyzed to determine at least one field of view and/or specific target location. The second optical device then interrogates the sample area at each determined target location, e.g., using Raman spectroscopy, to produce interrogation data. The processor determines whether the sample area includes predetermined biological or chemical particulates of interest based upon an analysis of the interrogation data and triggers an event such as an alarm or message if the predetermined biological or chemical particulates of interest are identified.
摘要翻译： 提供了一种生物和化学检测系统，用于检测和识别感兴趣的生物和/或化学颗粒物。 生物和化学检测系统包括收集器，第一光学装置，第二光学装置和处理器。 收集器被配置为将从流体流中吸取的微粒沉积到样品基底上以限定样品区域。 第一光学装置相对于样本区域的至少一部分导出第一数据，其被分析以确定至少一个视场和/或特定目标位置。 然后，第二光学装置在每个确定的目标位置询问样品区域，例如使用拉曼光谱法，产生询问数据。 处理器基于询问数据的分析来确定样本区域是否包括预期的生物或化学颗粒物，并且如果识别出预定的感兴趣的生物或化学颗粒物，则触发诸如报警或消息的事件。
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