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公开(公告)号：US5846485A
公开(公告)日：1998-12-08
申请号：US465928
申请日：1995-06-06
IPC分类号： C07D213/22 , C07D213/48 , C07D213/55 , C07D401/12 , C07D405/14 , C07D473/08 , C07F13/00 , C07F15/00 , C07H21/00 , C12Q1/04 , C12Q1/68 , G01N33/533 , G01N33/569 , G01N33/58 , G01N33/76 , G01N21/62
CPC分类号： C07D213/22 , C07D213/48 , C07D213/55 , C07D401/12 , C07D405/14 , C07D473/08 , C07F13/00 , C07F15/0026 , C07F15/0053 , C07H21/00 , C12Q1/04 , C12Q1/6816 , G01N33/533 , G01N33/569 , G01N33/582 , G01N33/76 , G01N2458/30 , Y10S435/808 , Y10S435/968 , Y10S435/975 , Y10S436/80 , Y10S436/805 , Y10S436/806 , Y10S436/807
摘要： A composition suitable for use in an ECL assay wherein electromagnetic radiation emitted by said composition is detected, which composition comprises (a) a metal-containing ECL moiety which, when oxidized by exposure to an effective amount of electrochemical energy, is capable of being converted to an excited state from which electromagnetic radiation is emitted upon exposure of the excited ECL moiety to conditions sufficient to induce said emission; (b) an amine or amine moiety which, when oxidized by exposure to an effective amount of electrochemical energy, forms a strong reducing agent in said composition; and (c) an electrolyte capable of functioning as a medium in which said ECL moiety and said amine or amine moiety can be oxidized by exposure to electrochemical energy.
摘要翻译： 一种适合用于ECL测定的组合物，其中检测到由所述组合物发射的电磁辐射，该组合物包含（a）含金属的ECL部分，当通过暴露于有效量的电化学能量而被氧化时，其能够被转化 到激发状态，当激发的ECL部分暴露于足以诱导所述发射的条件下时，电磁辐射从其发射; （b）胺或胺部分，当通过暴露于有效量的电化学能量而被氧化时，在所述组合物中形成强的还原剂; 和（c）能够作为介质的电解质，其中所述ECL部分和所述胺或胺部分可以通过暴露于电化学能被氧化。
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公开(公告)号：US06702986B1
公开(公告)日：2004-03-09
申请号：US09590398
申请日：2000-06-08
IPC分类号： G01N2176
CPC分类号： G01N33/533 , G01N21/76 , G01N27/305 , G01N33/582
摘要： The present invention relates to a composition suitable for use in an ECL assay wherein electromagnetic radiation emitted by the composition is detected. The composition includes an amine or amine moiety which, when oxidized by exposure to an effective amount of electrochemical energy, forms a strong reducing agent in the composition; and an electrolyte capable of functioning as a medium in which an ECL moiety and the amine or amine moiety can be oxidized by exposure to electrochemical energy.
摘要翻译： 本发明涉及一种适用于ECL测定的组合物，其中检测到由组合物发射的电磁辐射。 该组合物包括胺或胺部分，当通过暴露于有效量的电化学能量而被氧化时，在该组合物中形成强的还原剂; 以及能够起到ECL部分和胺或胺部分可通过暴露于电化学能而被氧化的介质的电解质。
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公开(公告)号：US6165729A
公开(公告)日：2000-12-26
申请号：US196315
申请日：1994-04-15
IPC分类号： C07D213/22 , C07D213/48 , C07D213/55 , C07D401/12 , C07D405/14 , C07D473/08 , C07F13/00 , C07F15/00 , C07H21/00 , C12Q1/04 , C12Q1/68 , G01N33/533 , G01N33/569 , G01N33/58 , G01N33/76 , C12Q1/70 , G01N21/76 , G01N33/53 , G01N33/566
CPC分类号： C07D213/22 , C07D213/48 , C07D213/55 , C07D401/12 , C07D405/14 , C07D473/08 , C07F13/00 , C07F15/0026 , C07F15/0053 , C07H21/00 , C12Q1/04 , C12Q1/6816 , G01N33/533 , G01N33/569 , G01N33/582 , G01N33/76 , G01N2458/30 , Y10S435/808 , Y10S435/968 , Y10S435/975 , Y10S436/80 , Y10S436/805 , Y10S436/806 , Y10S436/807
摘要： Electrochemiluminescent assay methods for determining an analyte of interest, which methods include forming one or more compositions which include, in the aggregate, (a) a sample to be tested for the analyte of interest, (b) a component capable of specifically binding with the analyte, (c) a label reagent which, when oxidized, is capable of electrochemiluminescence, (d) an amine which, when oxidized, forms a reducing agent, and (e) an electrolyte solution; subjecting such a composition containing the sample to conditions sufficient for specific binding to occur between the analyte of interest, if present, and one or more of the other components in the composition; thereafter, a composition containing the label reagent and amine, and reflecting the outcome of applying the aforementioned binding conditions, is exposed to conditions, such that both the label reagent and the amine are oxidized, the amine forms a reducing agent which interacts with the label reagent, electrochemiluminescence occurs, and the luminescence emitted from the label reagent is used to determine the analyte of interest.
摘要翻译： 用于确定感兴趣分析物的电化学发光测定方法，所述方法包括形成一种或多种组合物，所述组合物在总体上包括（a）待测试样品用于感兴趣的分析物，（b）能够与 分析物，（c）当氧化时能够进行电化学发光的标记试剂，（d）当被氧化形成还原剂时的胺，和（e）电解质溶液; 使这样的含有样品的组合物具有足够的特异性结合的条件，发生在感兴趣的分析物（如果存在）和组合物中的一种或多种其它组分之间; 此后，含有标记试剂和胺的组合物并反映施用上述结合条件的结果，暴露于标记试剂和胺被氧化的条件下，胺形成与标记物相互作用的还原剂 试剂，发生电化学发光，并且使用从标记试剂发射的发光来确定感兴趣的分析物。
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公开(公告)号：US06451225B1
公开(公告)日：2002-09-17
申请号：US08467232
申请日：1995-06-06
IPC分类号： C09K300
CPC分类号： C07D213/22 , C07D213/48 , C07D213/55 , C07D401/12 , C07D405/14 , C07D473/08 , C07F13/00 , C07F15/0026 , C07F15/0053 , C07H21/00 , C12Q1/04 , G01N21/76 , G01N27/305 , G01N33/533 , G01N33/569 , G01N33/582 , G01N33/76
摘要： A composition suitable for use in an ECL assay wherein electromagnetic radiation emitted by said composition is detected, which composition comprises (a) a metal-containing ECL moiety which, when oxidized by exposure to an effective amount of electrochemical energy, is capable of being converted to an excited state from which electromagnetic radiation is emitted upon exposure of the excited ECL moiety to conditions sufficient to induce said emission; (b) an amine or amine moiety which, when oxidized by exposure to an effective amount of electrochemical energy, forms a strong reducing agent in said composition; and (c) an electrolyte capable of functioning as a medium in which said ECL moiety and said amine or amine moiety can be oxidized by exposure to electrochemical energy.
摘要翻译： 一种适合用于ECL测定的组合物，其中检测到由所述组合物发射的电磁辐射，该组合物包含（a）含金属的ECL部分，当通过暴露于有效量的电化学能量而被氧化时，其能够被转化 被激发的状态从激发的ECL部分暴露于足以诱导所述发射的条件下发射电磁辐射;（b）胺或胺部分，当通过暴露于有效量的电化学能量而被氧化时形成强的 所述组合物中的还原剂; 和（c）能够作为介质的电解质，其中所述ECL部分和所述胺或胺部分可以通过暴露于电化学能被氧化。
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公开(公告)号：US06271041B1
公开(公告)日：2001-08-07
申请号：US08467936
申请日：1995-06-06
IPC分类号： G01N3348
CPC分类号： C07H21/00 , C07D473/08 , C07F15/0026 , C07F15/0053 , C12Q1/04 , C12Q1/6816 , G01N21/76 , G01N27/305 , G01N33/533 , G01N33/569 , G01N33/582 , G01N33/76 , Y10S436/805
摘要： A composition suitable for use in an ECL assay and kits therefor wherein electromagnetic radiation emitted by said composition is detected, which composition comprises (a) a metal-containing ECL moiety which, when oxidized by exposure to an effective amount of electrochemical energy, is capable of being converted to an excited state from which electromagnetic radiation is emitted upon exposure of the excited ECL moiety to conditions sufficient to induce said emission; (b) an amine or amine moiety which, when oxidized by exposure to an effective amount of electrochemical energy, forms a strong reducing agent in said composition; and (c) an electrolyte capable of functioning as a medium in which said ECL moiety and said amine or amine moiety can be oxidized by exposure to electrochemical energy.
摘要翻译： 一种适合用于ECL测定的组合物及其试剂盒，其中检测由所述组合物发射的电磁辐射，该组合物包含（a）含金属的ECL部分，当通过暴露于有效量的电化学能量而被氧化时， 被转化为激发状态，在激发的ECL部分暴露于足以诱导所述发射的条件下，从其发射电磁辐射; （b）胺或胺部分，当通过暴露于有效量的电化学能量而被氧化时，在所述组合物中形成强的还原剂; 和（c）能够作为介质的电解质，其中所述ECL部分和所述胺或胺部分可以通过暴露于电化学能被氧化。
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