公开(公告)号：US5766915A

公开(公告)日：1998-06-16

申请号：US380438

申请日：1995-01-30

申请人： Jonathan Knowles ,  Merja Penttila ,  Tuula Teeri ,  Helena Nevalainen ,  Paivi Lehtovaara-Helenius ,  Sirpa Aho ,  Sunee Nitisinprasert ,  Marja Paloheimo ,  Sirkka Keranen

发明人： Jonathan Knowles ,  Merja Penttila ,  Tuula Teeri ,  Helena Nevalainen ,  Paivi Lehtovaara-Helenius ,  Sirpa Aho ,  Sunee Nitisinprasert ,  Marja Paloheimo ,  Sirkka Keranen

IPC分类号： C12N1/19 ,  C12N9/42 ,  C12N15/62 ,  C12N15/81 ,  C12N15/56

CPC分类号： C12N9/2437 ,  C12N15/625 ,  C12N15/81 ,  C12Y302/01004 ,  C12Y302/01091 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/034

摘要： Functional derivatives of endoglucanase I (EGI), and the cloning and recombinant expression thereof, are described.

摘要翻译： 描述了内切葡聚糖酶I（EGI）的功能衍生物及其克隆和重组表达。

公开(公告)号：US5529919A

公开(公告)日：1996-06-25

申请号：US264492

申请日：1994-06-23

申请人： Jonathan Knowles ,  Merja Penttila ,  Tuula Teeri ,  Helena Nevalainen ,  Paivi Lehtovaara-Helenius

发明人： Jonathan Knowles ,  Merja Penttila ,  Tuula Teeri ,  Helena Nevalainen ,  Paivi Lehtovaara-Helenius

IPC分类号： C07H20060101 ,  C07H21/00 ,  C07K20060101 ,  C07K7/00 ,  C12N20060101 ,  C12N1/00 ,  C12N1/19 ,  C12N1/21 ,  C12N9/00 ,  C12N9/42 ,  C12N15/00 ,  C12N15/56 ,  C12N15/62 ,  C12N15/81

CPC分类号： C12N9/2437 ,  C12N15/625 ,  C12N15/81 ,  C12Y302/01091 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/034 ,  Y10S435/945

摘要： Described are methods and means for the construction of strains of yeast capable of producing cellulolytic enzymes. This is achieved by the transfer of chromosomal genes or cDNA copies of mRNAs coding for cellulolytic enzymes isolated from the fungus Trichoderma reesei to yeast cells using recombinant DNA vectors capable of replicating in yeast. The correct expression of these cellulolytic genes in yeast leads to the production of cellulolytic enzymes which are secreted from the cell. This allows the yeast to hydrolyze 3-1,4-glucan substrates such as cellulose.

摘要翻译： 描述了构建能产生纤维素分解酶的酵母菌株的方法和手段。 这是通过使用能够在酵母中复制的重组DNA载体将编码从真菌里氏木霉分离的纤维素分解酶的mRNA的染色体基因或cDNA拷贝转移到酵母细胞来实现的。 酵母中这些纤维素分解基因的正确表达导致从细胞分泌的纤维素分解酶的产生。 这使得酵母能够水解3-1,4-葡聚糖底物如纤维素。

公开(公告)号：US4894338A

公开(公告)日：1990-01-16

申请号：US817942

申请日：1986-01-30

申请人： Jonathan Knowles ,  Merja Penttila ,  Tuula Teeri ,  Helena Nevalainen ,  Irma Salovuori ,  Paivi Lehtovaara-Helenius

发明人： Jonathan Knowles ,  Merja Penttila ,  Tuula Teeri ,  Helena Nevalainen ,  Irma Salovuori ,  Paivi Lehtovaara-Helenius

IPC分类号： C07H20060101 ,  C07H21/00 ,  C07K20060101 ,  C07K7/00 ,  C12N20060101 ,  C12N1/00 ,  C12N1/19 ,  C12N1/21 ,  C12N9/00 ,  C12N9/42 ,  C12N15/00 ,  C12N15/56 ,  C12N15/62 ,  C12N15/81

CPC分类号： C12N9/2437 ,  C12N15/625 ,  C12N15/81 ,  C12Y302/01091 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/034 ,  Y10S435/945

摘要： The full length DNA sequence encoding the mature cellobiohydrolase II(CHBII), as well as the DNA sequence encoding the CBHII signal sequence, have been isolated and cloned from the fungus Trichoderma reesei. Recombinant vectors comprising these sequences, and yeast hosts transformed with such vectors are disclosed. Also disclosed are methods for constructing the vectors and hosts according to the invention, as well as methods for expressing the disclosed sequences. CBHII is involved in hydrolysis of cellulose.

公开(公告)号：US5393670A

公开(公告)日：1995-02-28

申请号：US95253

申请日：1993-07-23

申请人： Jonathan Knowles ,  Merja Penttila ,  Tuula Teeri ,  Helena M. K. Nevalainen ,  Paivi Lehtovaara-Helenius

发明人： Jonathan Knowles ,  Merja Penttila ,  Tuula Teeri ,  Helena M. K. Nevalainen ,  Paivi Lehtovaara-Helenius

IPC分类号： C07H20060101 ,  C07H21/00 ,  C07K20060101 ,  C07K7/00 ,  C12N20060101 ,  C12N1/00 ,  C12N1/19 ,  C12N1/21 ,  C12N9/00 ,  C12N9/42 ,  C12N15/00 ,  C12N15/56 ,  C12N15/62 ,  C12N15/81 ,  C12N15/52 ,  C12N15/70

CPC分类号： C12N9/2437 ,  C12N15/625 ,  C12N15/81 ,  C12Y302/01091 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/034 ,  Y10S435/945

摘要： Methods and means for the construction of strains of yeast capable of producing cellulolytic enzymes, achieved by the transfer of chromosomal genes or cDNA copies of mRNAs coding for cellulolytic enzymes isolated from the fungus Trichoderma reesei to yeast cells using recombinant DNA vectors capable of replicating in yeast. The correct expression of these cellulolytic genes in yeast leads to the production of cellulolytic enzymes which are secreted from the cell. This allows the yeast to hydrolyze 3-1,4-glucan substrates such as cellulose.

摘要翻译： 用于构建能够产生纤维素分解酶的酵母菌株的方法和手段，其通过使用能够在酵母中复制的重组DNA载体转染染色体基因或将编码从真菌里氏木霉分离的纤维素分解酶的mRNA的cDNA拷贝到酵母细胞 。 酵母中这些纤维素分解基因的正确表达导致从细胞分泌的纤维素分解酶的产生。 这使得酵母能够水解3-1,4-葡聚糖底物如纤维素。