摘要:
Methods and means for the construction of strains of yeast capable of producing cellulolytic enzymes, achieved by the transfer of chromosomal genes or cDNA copies of mRNAs coding for cellulolytic enzymes isolated from the fungus Trichoderma reesei to yeast cells using recombinant DNA vectors capable of replicating in yeast. The correct expression of these cellulolytic genes in yeast leads to the production of cellulolytic enzymes which are secreted from the cell. This allows the yeast to hydrolyze 3-1,4-glucan substrates such as cellulose.
摘要:
Described are methods and means for the construction of strains of yeast capable of producing cellulolytic enzymes. This is achieved by the transfer of chromosomal genes or cDNA copies of mRNAs coding for cellulolytic enzymes isolated from the fungus Trichoderma reesei to yeast cells using recombinant DNA vectors capable of replicating in yeast. The correct expression of these cellulolytic genes in yeast leads to the production of cellulolytic enzymes which are secreted from the cell. This allows the yeast to hydrolyze 3-1,4-glucan substrates such as cellulose.
摘要:
The full length DNA sequence encoding the mature cellobiohydrolase II(CHBII), as well as the DNA sequence encoding the CBHII signal sequence, have been isolated and cloned from the fungus Trichoderma reesei. Recombinant vectors comprising these sequences, and yeast hosts transformed with such vectors are disclosed. Also disclosed are methods for constructing the vectors and hosts according to the invention, as well as methods for expressing the disclosed sequences. CBHII is involved in hydrolysis of cellulose.
摘要:
Described are methods and systems for dynamically reallocating memory amongst virtual machines executing within a virtualization environment. A computer can execute a virtualization environment that can include one or more virtual machines and that can include a memory manager. The memory manager can dynamically reallocate memory by identifying a maximum and minimum memory value for each virtual machine, determining a target memory value for each virtual machine using the maximum and minimum memory value, and identifying one or more virtual machines that have an actual memory usage value that is less than the target memory value calculated for those virtual machines. To re-allocate the memory, the memory manager can allocate additional memory to the identified virtual machines by inflating a balloon driver, then de-allocate the additional memory, and reallocate the de-allocated, additional memory to other virtual machines within the virtualization environment.
摘要:
Enzyme preparations enriched in hemicellulase-, pectin-, and/or lignin-degrading enzymes are described which are also partially or completely deficient in cellulase degrading activity. Such preparations may be utilized in an crude, unpurified form and are especially useful in the production of pulp and paper.
摘要:
A technique manages a virtual hard disk tree in a computing system executing a hypervisor to provide a client virtualization environment. The technique involves linking, by a client executing within a control virtual machine of the client virtualization environment, a first delta image of a virtual hard disk generated later in time to a base image of the virtual hard disk. The technique further involves modifying contents of the first delta image, the base image, and a second delta image which is linked to the base image; and deleting the second delta image after modifying the contents of the first delta image, the base image, and the second delta image. The base image and the first delta image, together with additional delta images of the virtual hard disk comprise a tree of images of the virtual hard disk.
摘要:
A technique manages a virtual hard disk tree in a computing system executing a hypervisor to provide a client virtualization environment. The technique involves linking, by a client executing within a control virtual machine of the client virtualization environment, a first delta image of a virtual hard disk generated later in time to a base image of the virtual hard disk. The technique further involves modifying contents of the first delta image, the base image, and a second delta image which is linked to the base image; and deleting the second delta image after modifying the contents of the first delta image, the base image, and the second delta image. The base image and the first delta image, together with additional delta images of the virtual hard disk comprise a tree of images of the virtual hard disk.
摘要:
Described are methods and systems for dynamically reallocating memory amongst virtual machines executing within a virtualization environment. A computer can execute a virtualization environment that can include one or more virtual machines and that can include a memory manager. The memory manager can dynamically reallocate memory by identifying a maximum and minimum memory value for each virtual machine, determining a target memory value for each virtual machine using the maximum and minimum memory value, and identifying one or more virtual machines that have an actual memory usage value that is less than the target memory value calculated for those virtual machines. To re-allocate the memory, the memory manager can allocate additional memory to the identified virtual machines by inflating a balloon driver, then de-allocate the additional memory, and reallocate the de-allocated, additional memory to other virtual machines within the virtualization environment.