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公开(公告)号：US20110053218A1
公开(公告)日：2011-03-03
申请号：US12809396
申请日：2008-12-16
摘要： A multiple promoter platform for expression of a protein or multiple and different proteins in a microorganism comprising two or more expression vectors having a nucleic acid molecule encoding a protein to be expressed, or having different nucleic acid molecules encoding different proteins to be expressed, wherein each vector has a different promoter operably linked to the nucleic acid molecule, and uses of the multiple promoter platform to produce recombinant proteins.
摘要翻译： 用于在微生物中表达蛋白质或多种不同蛋白质的多重启动子平台，其包含两个或更多个表达载体，所述表达载体具有编码要表达的蛋白质的核酸分子，或具有编码不同蛋白质的不同核酸分子，其中每个 载体具有与核酸分子可操作地连接的不同启动子，以及多重启动子平台用于产生重组蛋白质的用途。
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公开(公告)号：US07771974B2
公开(公告)日：2010-08-10
申请号：US10362829
申请日：2001-08-29
CPC分类号： C12N15/1027 , C12Q1/6844 , C12Q2525/179 , C12Q2525/161
摘要： A method for gene shuffling to form a mutant or chimeric gene, the method comprising: (a) assigning one or more segments of one or more genes based on regions of encoded amino acid sequence; (b) amplifying the one or more assigned segments of the gene using primers specific for each segment; and (c) causing recombination of the one or more amplified segments to form a mutant or chimeric gene. An oligonucleotide primer suitable for use in gene shuffling, the primer having a non-degenerate core based on a segment or template of a gene to be amplified, and the core being flanked by both 5′ and 3′ degenerate ends.
摘要翻译： 一种用于基因改组以形成突变体或嵌合基因的方法，所述方法包括：（a）基于编码的氨基酸序列的区域分配一个或多个基因的一个或多个片段; （b）使用对每个片段特异的引物扩增该基因的一个或多个指定片段; 和（c）引起所述一个或多个扩增片段的重组以形成突变体或嵌合基因。 适用于基因改组的寡核苷酸引物，具有基于待扩增基因的片段或模板的非简并核心的引物，以及5'和3'简并末端两侧的核心。
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公开(公告)号：US20080233612A1
公开(公告)日：2008-09-25
申请号：US10573343
申请日：2004-09-23
摘要： An isolated mutant Ophiostoma species having enhanced protein excretion capability as compared with its parent strain cultured under similar conditions.
摘要翻译： 与在相似条件下培养的亲本菌株相比，具有增强的蛋白质排泄能力的分离的突变体Ophiostoma物种。
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公开(公告)号：US07517685B2
公开(公告)日：2009-04-14
申请号：US10498549
申请日：2002-12-13
IPC分类号： C12N5/08
CPC分类号： C07K14/37 , C12N15/10 , C12N15/1034 , C12N15/80 , C12Q1/68 , C12Q1/6895 , C12Q2600/158
摘要： The invention is directed to a method for obtaining a promoter from an organism using a proteomics approach, isolated promoters from fungal species and uses of the isolated promoters.
摘要翻译： 本发明涉及使用蛋白质组学方法从生物体获得启动子的方法，来自真菌种类的分离的启动子和分离的启动子的用途。
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5.发明授权Yeast strains producing cellulolytic enzymes and methods and means for constructing them 失效生产纤维素分解酶的酵母菌株及其构建方法和手段
公开(公告)号：US5766915A
公开(公告)日：1998-06-16
申请号：US380438
申请日：1995-01-30
申请人： Jonathan Knowles , Merja Penttila , Tuula Teeri , Helena Nevalainen , Paivi Lehtovaara-Helenius , Sirpa Aho , Sunee Nitisinprasert , Marja Paloheimo , Sirkka Keranen
发明人： Jonathan Knowles , Merja Penttila , Tuula Teeri , Helena Nevalainen , Paivi Lehtovaara-Helenius , Sirpa Aho , Sunee Nitisinprasert , Marja Paloheimo , Sirkka Keranen
CPC分类号： C12N9/2437 , C12N15/625 , C12N15/81 , C12Y302/01004 , C12Y302/01091 , C07K2319/00 , C07K2319/02 , C07K2319/034
摘要： Functional derivatives of endoglucanase I (EGI), and the cloning and recombinant expression thereof, are described.
摘要翻译： 描述了内切葡聚糖酶I（EGI）的功能衍生物及其克隆和重组表达。
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公开(公告)号：US5665585A
公开(公告)日：1997-09-09
申请号：US385370
申请日：1995-02-07
申请人： Tuula Torkkeli , Vesa Joutsjoki , Helena Torkkeli , Arja Vainio , Richard Fagerstrom , Sirpa Aho , Matti Korhola , Helena Nevalainen
发明人： Tuula Torkkeli , Vesa Joutsjoki , Helena Torkkeli , Arja Vainio , Richard Fagerstrom , Sirpa Aho , Matti Korhola , Helena Nevalainen
CPC分类号： C12N9/2428 , C12N15/80
摘要： The invention is directed to amino acid and DNA sequences of a unique glucoamylase P that has a high debranching activity, a Trichoderma host cell, transformed with such sequences, the expression of such recombinant glucoamylase P, and the industrial uses for the recombinant enzyme and hosts transformed therewith.
摘要翻译： 本发明涉及具有高脱支活性的独特葡糖淀粉酶P的氨基酸和DNA序列，用这些序列转化的木霉属宿主细胞，这种重组葡糖淀粉酶P的表达，以及重组酶和宿主的工业用途 随之改变。
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公开(公告)号：US20050221474A1
公开(公告)日：2005-10-06
申请号：US10498549
申请日：2002-12-13
IPC分类号： C07K14/37 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/10 , C12N15/31 , C12N15/63 , C12N15/80 , C12Q1/68 , C12N15/00
CPC分类号： C07K14/37 , C12N15/10 , C12N15/1034 , C12N15/80 , C12Q1/68 , C12Q1/6895 , C12Q2600/158
摘要： The invention is directed to a method for obtaining a promoter from an organism using a proteomics approach, isolated promoters from fungal species and uses of the isolated promoters.
摘要翻译： 本发明涉及使用蛋白质组学方法从生物体获得启动子的方法，来自真菌种类的分离的启动子和分离的启动子的用途。
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公开(公告)号：US06011147A
公开(公告)日：2000-01-04
申请号：US466047
申请日：1995-06-06
申请人： Tiina Hannele Nakari , Maija-Leena Onnela , Marja Hannele Ilmen , Kaisu Milja Helena Nevalainen , Merja Elisa Penttila
发明人： Tiina Hannele Nakari , Maija-Leena Onnela , Marja Hannele Ilmen , Kaisu Milja Helena Nevalainen , Merja Elisa Penttila
IPC分类号： A23K1/165 , C07K14/37 , C12N9/26 , C12N9/42 , C12N9/64 , C12N9/80 , C12N15/80 , C12N15/90 , C07H21/04 , C12N1/14 , C12N1/15 , C12N15/63
CPC分类号： C12N9/2408 , A23K20/189 , C07K14/37 , C12N15/80 , C12N15/90 , C12N9/2428 , C12N9/2434 , C12N9/2471 , C12N9/6481 , C12N9/80 , C12Y302/01023 , C12Y302/01091 , C12Y302/0115 , C07K2319/00 , C07K2319/02 , Y10S435/911 , Y10S435/913 , Y10S435/925 , Y10S435/929 , Y10S435/931 , Y10S435/945
摘要： A method is described for the identification and cloning of promoters that express under a defined environmental condition, such as growth in glucose medium. Using this method, five Trichodermal promoters capable of the high expression of operably linked coding sequences are identified, one of which is the promoter for T. reesei tef1. Also provided are altered cbh1 promoters, altered so that glucose no longer represses expression from such promoter. The invention further provides vectors and hosts that utilize such promoters, and unique fungal enzyme compositions from such hosts.
摘要翻译： 描述了用于鉴定和克隆在限定的环境条件下表达的启动子的方法，例如葡萄糖培养基中的生长。 使用这种方法，鉴定了能够高度表达可操作连接的编码序列的五个Trichodermal启动子，其中之一是里氏木霉tef1的启动子。 还提供改变的cbh1启动子，改变，使得葡萄糖不再抑制这种启动子的表达。 本发明还提供了利用这种启动子的载体和宿主，以及来自这种宿主的独特的真菌酶组合物。
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9.发明授权Enzyme preparations with recombinantly-altered cellulose profiles and methods for their production 失效具有重组改变的纤维素酶分布的酶制剂及其生产方法
公开(公告)号：US5298405A
公开(公告)日：1994-03-29
申请号：US524308
申请日：1990-05-16
IPC分类号： A23K1/165 , A23K3/03 , C12N11/18 , C12N15/00 , C12N15/52 , C12N15/56 , D21C5/00 , C12N15/80 , C12N15/90
CPC分类号： C12Y302/01 , A23K20/189 , A23K30/18 , C12N11/18 , C12N15/00 , C12N15/52 , D21C5/005
摘要： Enzyme preparations enriched in hemicellulase-, pectin-, and/or lignin-degrading enzymes are described which are also partially or completely deficient in cellulase degrading activity. Such preparations may be utilized in an crude, unpurified form and are especially useful in the production of pulp and paper.
摘要翻译： 描述了富含半纤维素酶，果胶和/或木质素降解酶的酶制剂，其也部分或完全缺乏纤维素酶降解活性。 这种制剂可以以粗制，未纯化的形式使用，并且特别可用于制造纸浆和纸。
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公开(公告)号：US5837515A
公开(公告)日：1998-11-17
申请号：US121436
申请日：1993-09-16
IPC分类号： A23K1/165 , A23K3/03 , C12N1/21 , C12N9/24 , C12N11/18 , C12N15/00 , C12N15/52 , C12N15/56 , C12N15/80 , D21C5/00 , C12N15/63
CPC分类号： C12Y302/01032 , A23K20/189 , A23K30/18 , C12N11/18 , C12N15/00 , C12N15/52 , C12N15/80 , C12N9/248 , C12Y302/01 , C12Y302/01008 , D21C5/005
摘要： The structure of the T. reesei xln1 and xln2 genes and the primaru structure of proteins are described. Enzyme preparations enriched in hemicellulase enzymes are described. Such enzyme preparations may also be partially or completely deficient in cellulose degrading activity. Such preparations may be utilized in an crude, unpurified form and are especially useful in the production of pulp and paper.
摘要翻译： 描述了里氏木霉xln1和xln2基因的结构和蛋白质的primaru结构。 描述了富含半纤维素酶的酶制剂。 这样的酶制剂也可能部分或完全缺乏纤维素降解活性。 这种制剂可以以粗制，未纯化的形式使用，并且特别可用于制造纸浆和纸。
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