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    Procedure for controlling the enumeration of the absolute count of cells or other particles present in a sample
    2.
    发明授权
    Procedure for controlling the enumeration of the absolute count of cells or other particles present in a sample 失效
    用于控制样品中存在的细胞或其他颗粒的绝对计数的计数的程序

    公开(公告)号：US06913901B2

    公开(公告)日：2005-07-05

    申请号：US10078558

    申请日：2002-02-14

    申请人： José Alberto Orfao de Matos Correia e Vale

    发明人： José Alberto Orfao de Matos Correia e Vale

    IPC分类号： G01N15/10 ,  G01N15/14 ,  C12Q1/02 ,  C12P35/06

    CPC分类号： G01N15/1012 ,  G01N2015/1406

    摘要： Procedure for controlling the enumeration of the absolute count of cells (or other particles) present in a sample. The procedure consists of the following: to prepare, in known quantities and proportions, a mixture or stock solution of two or more populations of reference particles of differing characteristics; to add a known quantity of this mixture of reference particles to a known volume of the sample which contains the cells (or other particles) to be counted; to measure, in a flow cytometer, the sample containing both the cells (or other particles), the events of which are to be counted, and the mixture of different populations of reference particles; to calculate the absolute number of cells (or other particles) present in the sample to be counted and; to check that the proportion between the different reference particles present in the sample measured concurs with the proportion that exists between them in the initial mixture or stock solution which contained the different populations of reference particles prior to adding it to the sample.

    摘要翻译： 用于控制样品中存在的细胞（或其他颗粒）的绝对计数的计数的程序。 该方法包括以下步骤：以已知的量和比例制备具有不同特征的两个或更多个参比颗粒群的混合物或储备溶液; 将已知量的参考颗粒混合物添加到包含要计数的细胞（或其他颗粒）的样品的已知体积中; 在流式细胞仪中测量含有细胞（或其他颗粒），其事件将被计数的样品和不同参考颗粒群体的混合物; 计算待计数样品中存在的细胞（或其他颗粒）的绝对数; 以检查测量样品中存在的不同参考颗粒之间的比例与添加到样品中之前含有参考颗粒群的初始混合物或储备溶液中之间存在的比例是否一致。

    METHODS AND KITS FOR DETECTING IGE-EXPRESSING B CELLS
    3.
    发明申请
    METHODS AND KITS FOR DETECTING IGE-EXPRESSING B CELLS 有权
    用于检测I型表达B细胞的方法和试剂盒

    公开(公告)号：US20140315749A1

    公开(公告)日：2014-10-23

    申请号：US14358287

    申请日：2011-11-15

    申请人： Menno Cornelis van Zelm ,  Jacobus Johannes Maria van Dongen ,  José Alberto Orfao de Matos Correia e Vale

    发明人： Menno Cornelis van Zelm ,  Jacobus Johannes Maria van Dongen ,  José Alberto Orfao de Matos Correia e Vale

    IPC分类号： G01N33/569 ,  G01N33/58

    CPC分类号： G01N33/56972 ,  G01N33/564 ,  G01N33/582 ,  G01N33/6854 ,  G01N2800/24

    摘要： The invention relates to the field of medical diagnostics. In particular, it relates method and kits for identification and classification of IgE-related diseases, e.g. Type I hypersensitivity, as well as for monitoring of treatment efficacy, for instance anti-IgE therapy. Provided is a multi-color flow cytometric method for analyzing memory B cell and plasma cell subsets in a biological sample, comprising staining the sample with a panel of fluorochrome-conjugated antibodies comprising antibodies against IgM, IgA, IgG, IgD and IgE; an antibody against a B cell marker and an antibody against the CD38 antigen; subjecting the sample to flow cytometry and gating for lymphocytes based on forward scatter and side scatter pattern; gating the lymphocytes for expression of the B cell specific marker and CD38 to discriminate between CD38dim memory B cells and CD38hi plasma cells; and quantitating the IgE+ cells within the memory B cell population and/or the plasma cell population by the negative selection of cells expressing IgM, IgA, IgG and/or IgD and the positive selection of IgE expressing cells.

    摘要翻译： 本发明涉及医疗诊断领域。 特别地，它涉及用于鉴定和分类IgE相关疾病的方法和试剂盒，例如， I型超敏反应，以及用于监测治疗功效，例如抗IgE治疗。 提供了一种用于分析生物样品中的记忆B细胞和浆细胞亚群的多色流式细胞术方法，其包括用包含针对IgM，IgA，IgG，IgD和IgE抗体的抗体的荧光染料缀合的抗体片染色样品; 针对B细胞标记的抗体和针对CD38抗原的抗体; 基于前向散射和侧向散射图案对样品进行流式细胞术和淋巴细胞门控; 门控淋巴细胞以表达B细胞特异性标记物和CD38以区分CD38dim记忆B细胞和CD38hi浆细胞; 并通过表达IgM，IgA，IgG和/或IgD的细胞的阴性选择和IgE表达细胞的阳性选择来定量记忆B细胞群体和/或浆细胞群体内的IgE +细胞。