公开(公告)号：US5385841A

公开(公告)日：1995-01-31

申请号：US989363

申请日：1992-12-11

申请人： Jose D. Ortega ,  Jose A. Gil ,  Tomas V. Garcia ,  Juan F. Martin

发明人： Jose D. Ortega ,  Jose A. Gil ,  Tomas V. Garcia ,  Juan F. Martin

IPC分类号： C12N15/12 ,  C12N1/21 ,  C12N9/02 ,  C12N9/12 ,  C12N15/09 ,  C12N15/63 ,  C12N15/76 ,  C12P21/00 ,  C12P21/02 ,  C12R1/465 ,  C12N15/00 ,  C12N15/11

CPC分类号： C12N9/001 ,  C12N15/76 ,  C12N9/12 ,  C12Y113/11001 ,  C12Y113/11002 ,  C07K2319/02

摘要： There is disclosed a new calcium-regulated promoter to be used for increasing production of extracellular enzymes, or heterologous polypeptides, a recombinant vector that includes the DNA sequence of the promoter operatively linked to a DNA encoding said enzyme or polypeptide and a host organism transformed with the recombinant vector that includes the promoter operatively linked to the DNA encoding said enzyme or polypeptide. The present invention further relates to Streptomyces expression systems and methods for expressing foreign DNA sequences in Streptomyces and for secreting to the surrounding medium polypeptides and proteins coded for by those foreign DNA sequences.

摘要翻译： 公开了用于增加细胞外酶或异源多肽的生产的新的钙调节启动子，重组载体，其包含与编码所述酶或多肽的DNA可操作地连接的启动子的DNA序列， 所述重组载体包括与编码所述酶或多肽的DNA可操作地连接的启动子。 本发明还涉及链霉菌表达系统和用于在链霉菌中表达外源DNA序列的方法，以及用于分泌周围培养基的多肽和由那些外来DNA序列编码的蛋白质。

公开(公告)号：US5122595A

公开(公告)日：1992-06-16

申请号：US354265

申请日：1989-05-19

申请人： Antonio D. Ortega ,  Jose A. Gil ,  Tomas V. Garcia ,  Juan F. Martin

发明人： Antonio D. Ortega ,  Jose A. Gil ,  Tomas V. Garcia ,  Juan F. Martin

IPC分类号： C12N15/03 ,  C07K14/36 ,  C12N15/00 ,  C12N15/31 ,  C12N15/52 ,  C12N15/67 ,  C12N15/76

CPC分类号： C07K14/36

摘要： The cloning and characterization of a newly isolated gene, referred to as saf (secondary metabolism activation factor) is disclosed. This gene encodes a new amino acid polypeptide, referred to as the SAF polypeptide, which directly or indirectly modulates the expression of extracellular enzymes in Steptomyces. DNA units or fragments which encode the SAF polypeptide are also disclosed, as are vectors containing said DNA, host organisms transformed with such vectors, and processes for preparing extracellular enzymes or heterologous polypeptides by culturing such host organisms.

摘要翻译： 公开了新分离的基因的克隆和表征，被称为saf（次级代谢活化因子）。 该基因编码一种称为SAF多肽的新的氨基酸多肽，其直接或间接调节步进样细胞中的细胞外酶的表达。 还公开了编码SAF多肽的DNA单元或片段，以及含有所述DNA的载体，用这些载体转化的宿主生物体，以及通过培养这些宿主生物体制备细胞外酶或异源多肽的方法。

公开(公告)号：US5118617A

公开(公告)日：1992-06-02

申请号：US410706

申请日：1989-09-22

申请人： Antonio D. Ortega ,  Jose A. Gil ,  Tomas V. Garcia ,  Juan F. Martin

发明人： Antonio D. Ortega ,  Jose A. Gil ,  Tomas V. Garcia ,  Juan F. Martin

IPC分类号： C12N15/09 ,  C07K14/00 ,  C07K14/36 ,  C07K14/37 ,  C07K14/41 ,  C12N20060101 ,  C12N1/21 ,  C12N15/11 ,  C12N15/31 ,  C12N15/63 ,  C12N15/67 ,  C12P1/00 ,  C12P21/02 ,  C12R1/465

CPC分类号： C07K14/36 ,  Y10S435/886

摘要： The cloning and characterization of a newly isolated gene, referred to as saf (secondary metabolism activation factor) is disclosed. This gene encodes a new amino acid polypeptide, referred to as the SAF polypeptide, which directly or indirectly modulates the expression of extracellular enzymes in Streptomyces. DNA units or fragments which encode the SAF polypeptides are also disclosed, as are vectors containing said DNA, host organisms transformed with such vectors, and processes for preparing extracellular enzymes or heterologous polypeptides by culturing such host organisms.

摘要翻译： 公开了新分离的基因的克隆和表征，被称为saf（次级代谢活化因子）。 该基因编码一种称为SAF多肽的新的氨基酸多肽，其直接或间接调节链霉菌中细胞外酶的表达。 还公开了编码SAF多肽的DNA单元或片段，以及含有所述DNA的载体，用这些载体转化的宿主生物体，以及通过培养这些宿主生物体制备细胞外酶或异源多肽的方法。