公开(公告)号：US4182656A

公开(公告)日：1980-01-08

申请号：US722270

申请日：1976-09-10

申请人： Joseph E. Ahnell ,  Rodney L. Broman ,  John R. Waters

发明人： Joseph E. Ahnell ,  Rodney L. Broman ,  John R. Waters

IPC分类号： C12M1/34 ,  C12Q1/16 ,  C12K1/04 ,  G01N27/78 ,  G01N33/16

CPC分类号： C12Q1/16 ,  C12M41/34 ,  C12M41/36

摘要： A sample of material to be tested for the presence of biologically active agents, such as bacteria, is introduced into a sealable container partially filled with a culture medium comprising a .sup.13 C-labeled fermentable substrate, the remainder of the container being filled with a culture gas, the container and its contents are subjected to conditions conducive to biological activity for a predetermined period sufficient for fermentation of the medium to produce carbon dioxide after which the ratio of .sup.13 CO.sub.2 to .sup.12 CO.sub.2 in the culture gas in the container is determined and compared to the initial ratio of .sup.13 CO.sub.2 to .sup.12 CO.sub.2 in the culture gas in order to detect any differences indicating the presence of biologically active agents in the sample.

摘要翻译： 将要测试的生物活性剂如细菌的待测试材料样品引入部分填充有包含13C标记的可发酵底物的培养基的可密封容器中，剩余的容器中填充培养气体 容器及其内容物经受有利于生物活性的条件一段足以发酵培养基以产生二氧化碳的预定时间，在此之后确定容器中培养气体中的13CO2至12CO2的比例，并将其与初始 比例为13CO2至12CO2，以检测指示样品中存在生物活性剂的任何差异。

公开(公告)号：US4073691A

公开(公告)日：1978-02-14

申请号：US717351

申请日：1976-08-24

申请人： Joseph E. Ahnell ,  Rodney L. Broman ,  John R. Waters

发明人： Joseph E. Ahnell ,  Rodney L. Broman ,  John R. Waters

IPC分类号： C12Q1/04 ,  C12K1/04

CPC分类号： C12Q1/04 ,  C12Q2304/46 ,  Y10S435/803 ,  Y10S435/807 ,  Y10S435/874

摘要： A sample of material to be tested for the presence of biologically active agents, such as bacteria, is introduced into a sealable container partially filled with a culture medium; the remainder of the container being filled with a culture gas, the container and its contents are subjected to conditions conducive to biological activity for a predetermined period sufficient for fermentation of the medium to produce at least one gaseous product after which the character of the culture gas in the container is determined and compared to the initial character of the culture gas in order to detect any differences caused by changes in the composition of the culture gas indicating the presence or absence of biologically active agents in the sample.

摘要翻译： 将要测试的生物活性剂如细菌的待测试材料样品引入部分填充有培养基的可密封容器中; 容器的剩余部分填充有培养气体，容器及其内容物经受有利于生物活性的条件达预定时间，足以使培养基发酵以产生至少一种气态产物，之后培养气体的特性 在容器中确定并与培养气体的初始特性进行比较，以便检测由样品中存在或不存在生物活性剂的培养气体组成变化引起的任何差异。

公开(公告)号：US4073694A

公开(公告)日：1978-02-14

申请号：US673359

申请日：1976-04-05

申请人： Dennis Buda ,  Rodney L. Broman ,  John R. Waters

发明人： Dennis Buda ,  Rodney L. Broman ,  John R. Waters

IPC分类号： C12Q1/18 ,  C12K1/00

CPC分类号： C12Q1/18 ,  Y10S435/873

摘要： A method of measuring the level of an aminoglycoside antibiotic, such as gentamicin, tobramycin or amikacin, in blood serum or plasma comprising incubating an aliquot of blood serum or plasma from a human patient being treated with an aminoglycoside antibiotic in a urea-containing culture medium with a susceptible strain of an adaptive urease producing microorganism corresponding to Proteus rettgeri JLI 03, ATCC No. 31168.

摘要翻译： 一种测量血清或血浆中氨基糖苷类抗生素（如庆大霉素，妥布霉素或阿米卡星）的含量的方法，包括在含尿素培养基中用氨基糖苷类抗生素处理的人类血清或血浆的等分试样 具有与Proteus rettgeri JLI 03，ATCC No.31168相对应的适应性脲酶产生微生物的敏感菌株。

公开(公告)号：US4769332A

公开(公告)日：1988-09-06

申请号：US773740

申请日：1985-09-06

申请人： Salman H. Siddiqi ,  Rodney L. Broman

发明人： Salman H. Siddiqi ,  Rodney L. Broman

IPC分类号： C12N1/38

CPC分类号： C12N1/38 ,  Y10S435/863

摘要： A growth medium for mycobacteria is provided which has enhanced growth properties. The growth medium contains an effective amount of polyoxyethylene stearate and an albumin.

摘要翻译： 提供了分枝杆菌的生长培养基，其具有增强的生长特性。 生长培养基含有有效量的聚氧乙烯硬脂酸酯和白蛋白。