公开(公告)号：US20120082361A1

公开(公告)日：2012-04-05

申请号：US12895273

申请日：2010-09-30

申请人： Julian BURKE ,  Alasdair ROBERTSON ,  Xiao ZHANG

发明人： Julian BURKE ,  Alasdair ROBERTSON ,  Xiao ZHANG

IPC分类号： G06K9/00 ,  C12M1/34 ,  G01N33/68

CPC分类号： G01N33/5044

摘要： In one aspect the present invention provides a method for selecting a cell or cell colony which produces a polypeptide of interest, comprising a) providing a medium comprising cells and a detection agent, wherein the detection agent is associated with a detectable signal and the detection agent is capable of binding to the polypeptide of interest; b) providing a solid phase having a capture agent disposed thereon, wherein the capture agent is capable of binding to the polypeptide of interest; c) contacting the medium with the solid phase; d) detecting the signal associated with the detection agent; and e) selecting a cell or cell colony associated with the signal, wherein presence of the signal is indicative of a cell or cell colony which produces the polypeptide of interest.

摘要翻译： 在一个方面，本发明提供了选择产生感兴趣多肽的细胞或细胞集落的方法，其包括a）提供包含细胞和检测剂的培养基，其中所述检测剂与可检测信号相关联，并且所述检测剂 能够结合目的多肽; b）提供其上设置有捕获剂的固相，其中捕获剂能够结合感兴趣的多肽; c）使介质与固相接触; d）检测与检测剂相关的信号; 和e）选择与所述信号相关的细胞或细胞集落，其中所述信号的存在指示产生感兴趣多肽的细胞或细胞集落。

公开(公告)号：US20240218342A1

公开(公告)日：2024-07-04

申请号：US18556642

申请日：2022-04-25

申请人： Lai-Xi WANG ,  Xiao ZHANG

发明人： Lai-Xi WANG ,  Xiao ZHANG

IPC分类号： C12N9/40 ,  A61K38/00 ,  C07H17/00 ,  C12N9/24

CPC分类号： C12N9/2465 ,  C07H17/00 ,  C12N9/2402 ,  C12Y302/01022 ,  C12Y302/01096 ,  A61K38/00

摘要： The present disclosure provides compounds useful for enzymatic glycan remodeling of a glycoprotein. Also provided is a method for remodeling a glycoprotein using M6P-glycan oxazolines in a one-pot deglycosylation/transglycosylation process, which may enable site selective M6P-glycan remodeling of glycoproteins to obtain homogeneous products. The remodeled glycoprotein (such as a recombinant human acid α-glucosidase) may have enhanced affinity for the CI-MPR, increased uptake by a cell, and improved therapeutic efficacy compared to the original glycoprotein. A method of treating Pompe disease using a glycan remodeled lysosomal enzyme is also provided.

公开(公告)号：US20210161955A2

公开(公告)日：2021-06-03

申请号：US16682422

申请日：2019-11-13

申请人： Jian XU ,  Xiao ZHANG ,  Jing WANG ,  Ling ZHU ,  Beverly LUBIT

发明人： Jian XU ,  Xiao ZHANG ,  Jing WANG ,  Ling ZHU ,  Beverly LUBIT

IPC分类号： A61K35/17 ,  C12N5/00 ,  C12N5/0783 ,  A61P35/00

摘要： The present disclosure describes a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a cell product comprising an expanded and enriched population of superactivated cytokine killer T cells, and methods for manufacturing the cell product.